##gff-version 3
##sequence-region BBa_K176224 1 2388
BBa_K176224	.	engineered_region	1	724	.	+	0	ID=BBa_I13502;Name=BBa_I13502
BBa_K176224	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_K176224	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176224	.	CDS	19	699	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_K176224	.	CDS	19	693	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K176224	.	engineered_tag	700	724	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K176224	.	engineered_region	733	2388	.	+	0	ID=BBa_K176189;Name=BBa_K176189
BBa_K176224	.	engineered_region	1728	2388	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K176189
BBa_K176224	.	ribosome_entry_site	1728	1739	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176224	.	conserved_region	1732	1735	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176224	.	CDS	1746	2363	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176224	.	engineered_region	1746	2363	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176224	.	engineered_tag	2364	2388	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176224	.	CDS	1746	2324	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176224	.	tag	2325	2356	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176224	.	engineered_region	733	1719	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510;Parent=BBa_K176189
BBa_K176224	.	terminator	1622	1662	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_K176224	.	polyA_site	1649	1662	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176224	.	stem_loop	1629	1648	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176224	.	stop_codon	1655	1655	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176224	.	engineered_region	1622	1662	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176224	.	ribosome_entry_site	733	744	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_K176224	.	conserved_region	737	740	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176224	.	terminator	1534	1613	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_K176224	.	stem_loop	1545	1588	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176224	.	engineered_region	1534	1613	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176224	.	promoter	1671	1719	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_K176224	.	engineered_region	1671	1719	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K176224	.	non_covalent_binding_site	1695	1711	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K176224	.	non_covalent_binding_site	1671	1687	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K176224	.	promoter	1708	1713	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K176224	.	promoter	1685	1690	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K176224	.	CDS	751	1500	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_K176224	.	CDS	754	1461	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K176224	.	tag	1462	1494	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K176224	.	engineered_tag	1501	1525	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
>BBa_K176224
aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggtt
ccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaa
agttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtt
aaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcg
aagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg
taccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccg
gaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaa
ccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaa
cgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgc
tagtgtagatcgctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttg
aggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaa
gatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgctt
gcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggtta
gtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagct
tagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgag
gttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctg
agcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcaggga
tagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtgggg
aaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataac
gctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaa
atactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtctt
cgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataact
caaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtga
ttatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagt
cgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatat
ataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtat
taaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcct
attaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgat
agtgctagtgtagatctc