LOCUS BBa_K176225 3016 bp DNA linear 22-Dec-2024 DEFINITION PoPS->AID(week RBS)->mRFP->CI Inverter (cI-LVA+pCI)->luxI-LVA->PoPS ACCESSION BBa_K176225 VERSION BBa_K176225.1 FEATURES Location/Qualifiers misc_feature 7..10 /label=conserved RBS 1..14 /label=BBa_B0031 misc_feature 21..23 /label=Start misc_feature 615..620 /label=Stop CDS 21..614 /label=Activation-Induced (Cytidine) Deaminase CDS 21..620 /label=BBa_K176190 misc_feature 1..620 /label=BBa_K176191 misc_feature 633..636 /label=conserved RBS 629..640 /label=BBa_B0034 CDS 647..1321 /label=mrfp1 misc_feature 1328..1352 /label=Help:Barcodes CDS 647..1327 /label=BBa_E1010 misc_feature 629..1352 /label=BBa_I13502 misc_feature 1365..1368 /label=conserved RBS 1361..1372 /label=BBa_B0034 CDS 1382..2089 /label=cI lambda misc_feature 2090..2122 /label=LVA misc_feature 2129..2153 /label=Help:Barcodes CDS 1379..2128 /label=BBa_C0051 stem_loop 2173..2216 /label=stem_loop misc_feature 2162..2241 /label=BBa_B0010 terminator 2162..2241 /label=BBa_B0010 stem_loop 2257..2276 /label=T7 TE misc_feature 2283..2283 /label=stop polyA_site 2277..2290 /label=polya misc_feature 2250..2290 /label=BBa_B0012 terminator 2250..2290 /label=BBa_B0012 promoter 2336..2341 /label=-10 promoter 2313..2318 /label=-35 misc_feature 2323..2339 /label=OR1 misc_feature 2299..2315 /label=OR2 misc_feature 2299..2347 /label=BBa_R0051 promoter 2299..2347 /label=BBa_R0051 misc_feature 1361..2347 /label=BBa_Q04510 misc_feature 2360..2363 /label=conserved RBS 2356..2367 /label=BBa_B0034 misc_feature 2953..2984 /label=LVA CDS 2374..2952 /label=luxI misc_feature 2374..2991 /label=BBa_C0061 misc_feature 2992..3016 /label=Help:Barcodes CDS 2374..2991 /label=BBa_C0061 misc_feature 2356..3016 /label=BBa_C0261 misc_feature 1361..3016 /label=BBa_K176189 misc_feature 629..3016 /label=BBa_K176224 ORIGIN 1 tcacacagga aacctactag atggacagcc tcttgatgaa ccggaggaag tttctttacc 61 aattcaaaaa tgtccgctgg gctaagggtc ggcgtgagac ctacctgtgc tacgtagtga 121 agaggcgtga cagtgctaca tccttttcac tggactttgg ttatcttcgc aataagaacg 181 gctgccacgt ggaattgctc ttcctccgct acatctcgga ctgggaccta gaccctggcc 241 gctgctaccg cgtcacctgg ttcacctcct ggagcccctg ctacgactgt gcccgacatg 301 tggccgactt tctgcgaggg aaccccaacc tcagtctgag gatcttcacc gcgcgcctct 361 acttctgtga ggaccgcaag gctgagcccg aggggctgcg gcggctgcac cgcgccgggg 421 tgcaaatagc catcatgacc ttcaaagatt atttttactg ctggaatact tttgtagaaa 481 accatgaaag aactttcaaa gcctgggaag ggctgcatga aaattcagtt cgtctctcca 541 gacagcttcg gcgcatcctt ttgcccctgt atgaggttga tgacttacga gacgcatttc 601 gtactttggg actttaataa tactagagaa agaggagaaa tactagatgg cttcctccga 661 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca 721 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa 781 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt 841 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact 901 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt 961 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg 1021 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc 1081 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct 1141 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa 1201 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa 1261 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc 1321 ttaataacgc tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat 1381 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc 1441 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat 1501 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta 1561 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat 1621 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga 1681 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac 1741 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc 1801 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt 1861 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg 1921 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca 1981 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc 2041 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga 2101 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga 2161 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2221 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 2281 tgcgtttata tactagagta acaccgtgcg tgttgactat tttacctctg gcggtgataa 2341 tggttgctac tagagaaaga ggagaaatac tagatgacta taatgataaa aaaatcggat 2401 tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt 2461 aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat 2521 aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg 2581 cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt 2641 caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa 2701 aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata 2761 tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata 2821 gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat 2881 gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa 2941 gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag 3001 tgctagtgta gatctc //