##gff-version 3
##sequence-region BBa_K176225 1 3016
BBa_K176225	.	engineered_region	629	3016	.	+	0	ID=BBa_K176224;Name=BBa_K176224
BBa_K176225	.	engineered_region	1361	3016	.	+	0	ID=BBa_K176189;Name=BBa_K176189;Parent=BBa_K176224
BBa_K176225	.	engineered_region	1361	2347	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510;Parent=BBa_K176189
BBa_K176225	.	terminator	2250	2290	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_K176225	.	stem_loop	2257	2276	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176225	.	stop_codon	2283	2283	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176225	.	polyA_site	2277	2290	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176225	.	engineered_region	2250	2290	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176225	.	ribosome_entry_site	1361	1372	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_K176225	.	conserved_region	1365	1368	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176225	.	CDS	1379	2128	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_K176225	.	engineered_tag	2129	2153	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K176225	.	CDS	1382	2089	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K176225	.	tag	2090	2122	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K176225	.	terminator	2162	2241	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_K176225	.	stem_loop	2173	2216	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176225	.	engineered_region	2162	2241	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176225	.	promoter	2299	2347	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_K176225	.	promoter	2313	2318	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K176225	.	engineered_region	2299	2347	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K176225	.	promoter	2336	2341	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K176225	.	non_covalent_binding_site	2323	2339	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K176225	.	non_covalent_binding_site	2299	2315	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K176225	.	engineered_region	2356	3016	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K176189
BBa_K176225	.	CDS	2374	2991	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176225	.	engineered_region	2374	2991	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176225	.	tag	2953	2984	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176225	.	CDS	2374	2952	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176225	.	engineered_tag	2992	3016	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176225	.	ribosome_entry_site	2356	2367	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176225	.	conserved_region	2360	2363	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176225	.	engineered_region	629	1352	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_K176224
BBa_K176225	.	ribosome_entry_site	629	640	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_K176225	.	conserved_region	633	636	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176225	.	CDS	647	1327	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_K176225	.	engineered_tag	1328	1352	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K176225	.	CDS	647	1321	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K176225	.	engineered_region	1	620	.	+	0	ID=BBa_K176191;Name=BBa_K176191
BBa_K176225	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176191
BBa_K176225	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176225	.	CDS	21	620	.	+	0	ID=BBa_K176190;Name=BBa_K176190;Parent=BBa_K176191
BBa_K176225	.	CDS	21	614	.	+	0	ID=annotation2032941;Name=Activation-Induced (Cytidine) Deaminase;Parent=BBa_K176190
BBa_K176225	.	stop_codon	615	620	.	+	0	ID=annotation2032940;Name=Stop;Parent=BBa_K176190
BBa_K176225	.	start_codon	21	23	.	+	0	ID=annotation2032939;Name=Start;Parent=BBa_K176190
>BBa_K176225
tcacacaggaaacctactagatggacagcctcttgatgaaccggaggaagtttctttaccaattcaaaaatgtccgctg
ggctaagggtcggcgtgagacctacctgtgctacgtagtgaagaggcgtgacagtgctacatccttttcactggacttt
ggttatcttcgcaataagaacggctgccacgtggaattgctcttcctccgctacatctcggactgggacctagaccctg
gccgctgctaccgcgtcacctggttcacctcctggagcccctgctacgactgtgcccgacatgtggccgactttctgcg
agggaaccccaacctcagtctgaggatcttcaccgcgcgcctctacttctgtgaggaccgcaaggctgagcccgagggg
ctgcggcggctgcaccgcgccggggtgcaaatagccatcatgaccttcaaagattatttttactgctggaatacttttg
tagaaaaccatgaaagaactttcaaagcctgggaagggctgcatgaaaattcagttcgtctctccagacagcttcggcg
catccttttgcccctgtatgaggttgatgacttacgagacgcatttcgtactttgggactttaataatactagagaaag
aggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgt
taacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagtt
accaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaac
acccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaaga
cggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacc
aacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaag
acggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccac
ctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaa
gactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagt
gtagatcgctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgagga
cgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatg
gggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaa
aaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtat
gcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttaga
acctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttg
aaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagca
ggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagc
ggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaag
ttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctg
atagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatac
tagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtt
atcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaa
tgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattat
atgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtt
ttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataa
acacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaa
gttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctatta
atgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtg
ctagtgtagatctc