LOCUS BBa_K176226 3401 bp DNA linear 22-Dec-2024 DEFINITION Repressible cell density controller: PoPS->tetR-LVA->K176096 ACCESSION BBa_K176226 VERSION BBa_K176226.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..638 /label=tetR misc_feature 639..672 /label=SsrA misc_feature 679..703 /label=Help:Barcodes CDS 19..678 /label=BBa_C0040 misc_feature 1..703 /label=BBa_S0101 stem_loop 723..766 /label=stem_loop misc_feature 712..791 /label=BBa_B0010 terminator 712..791 /label=BBa_B0010 stem_loop 807..826 /label=T7 TE misc_feature 833..833 /label=stop polyA_site 827..840 /label=polya misc_feature 800..840 /label=BBa_B0012 terminator 800..840 /label=BBa_B0012 terminator 712..840 /label=BBa_B0015 misc_feature 1..840 /label=BBa_K176006 promoter 849..854 /label=-35 promoter 872..877 /label=-10 misc_feature 865..865 /label=c in J23115 misc_feature 869..869 /label=t in J23115 misc_feature 855..860 /label=NheI misc_feature 878..883 /label=NheI misc_feature 866..871 /label=AvrII promoter 849..883 /label=BBa_K176008 misc_feature 896..899 /label=conserved RBS 892..903 /label=BBa_B0034 misc_feature 1489..1520 /label=LVA CDS 910..1488 /label=luxI misc_feature 910..1527 /label=BBa_C0061 misc_feature 1528..1552 /label=Help:Barcodes CDS 910..1527 /label=BBa_C0061 misc_feature 892..1552 /label=BBa_C0261 stem_loop 1572..1615 /label=stem_loop misc_feature 1561..1640 /label=BBa_B0010 terminator 1561..1640 /label=BBa_B0010 stem_loop 1656..1675 /label=T7 TE misc_feature 1682..1682 /label=stop polyA_site 1676..1689 /label=polya misc_feature 1649..1689 /label=BBa_B0012 terminator 1649..1689 /label=BBa_B0012 terminator 1561..1689 /label=BBa_B0015 misc_feature 892..1689 /label=BBa_K082014 misc_feature 849..1689 /label=BBa_K176022 promoter 1698..1732 /label=BBa_J23101 misc_feature 1745..1748 /label=conserved RBS 1741..1752 /label=BBa_B0034 misc_feature 1759..1760 /label=prefix misc_feature 1932..1932 /label=T misc_feature 2250..2250 /label=A CDS 1759..2508 /label=luxR misc_feature 1759..2514 /label=BBa_C0062 misc_feature 2515..2539 /label=Help:Barcodes CDS 1759..2514 /label=BBa_C0062 stem_loop 2559..2602 /label=stem_loop misc_feature 2548..2627 /label=BBa_B0010 terminator 2548..2627 /label=BBa_B0010 stem_loop 2643..2662 /label=T7 TE misc_feature 2669..2669 /label=stop polyA_site 2663..2676 /label=polya misc_feature 2636..2676 /label=BBa_B0012 terminator 2636..2676 /label=BBa_B0012 misc_feature 1741..2676 /label=BBa_I0462 misc_feature 1698..2676 /label=BBa_K176025 misc_feature 2685..2737 /label=R0062 promoter 2704..2709 /label=-35 promoter 2726..2731 /label=-10 misc_feature 2738..2756 /label=tetR misc_feature 2737..2737 /label=+1 misc_feature 2685..2704 /label=luxR/HSL promoter 2685..2756 /label=BBa_K176000 misc_feature 1698..2756 /label=BBa_K176028 misc_feature 849..2756 /label=BBa_K176085 misc_feature 2771..2774 /label=conserved RBS 2765..2778 /label=BBa_B0031 misc_feature 2785..2949 /label=a part of lacZalpha (I732006) misc_feature 2785..2787 /label=Start misc_feature 2800..2800 /label=177bp and a in J32026 primer_bind 2805..2821 /label=M13 -20 primer primer_bind 2825..2848 /label=M13 -47 primer (K176059) primer_bind 2825..2841 /label=M13 -40 primer misc_feature 2949..2949 /label=g and 69bp in I732006 misc_feature 2956..2957 /label=at in K145151 misc_feature 2956..3261 /label=ccdB (K145151) misc_feature 3039..3039 /label=c in K145151 misc_feature 3259..3264 /label=Stop primer_bind 2972..2999 /label=VRccdB primer (K176058) CDS 2785..3258 /label=lacZalpha-ccdB CDS 2785..3264 /label=BBa_K176003 stem_loop 3284..3327 /label=stem_loop misc_feature 3273..3352 /label=BBa_B0010 terminator 3273..3352 /label=BBa_B0010 stem_loop 3368..3387 /label=T7 TE misc_feature 3394..3394 /label=stop polyA_site 3388..3401 /label=polya misc_feature 3361..3401 /label=BBa_B0012 terminator 3361..3401 /label=BBa_B0012 misc_feature 2765..3401 /label=BBa_K176063 misc_feature 849..3401 /label=BBa_K176096 ORIGIN 1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 841 tactagagtt tatagctagc tcagtcctag gtacaatgct agctactaga gaaagaggag 901 aaatactaga tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag 961 tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta 1021 gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga atatatttat 1081 gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat 1141 tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct 1201 aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct 1261 gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt 1321 attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa 1381 gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt 1441 gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac 1501 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 1561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1621 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1681 gcgtttatat actagagttt acagctagct cagtcctagg tattatgcta gctactagag 1741 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat 1801 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa 1861 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct 1921 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat 1981 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg 2041 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa 2101 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga 2161 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg 2221 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata 2281 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca 2341 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc 2401 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt 2461 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga 2521 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga 2581 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 2641 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac 2701 aggtttacgc aagaaaatgg tttgttatag tcgaatatcc ctatcagtga tagagatact 2761 agagtcacac aggaaaccta ctagatgacc atgattacgg attcactggc cgtcgtttta 2821 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 2881 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 2941 cgcagcctat acgtacggca gtttaaggtt tacacctata aaagagagag ccgttatcgt 3001 ctgtttgtgg atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc 3061 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat 3121 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt 3181 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 3241 ctgatgttct ggggaatata ataatactag agccaggcat caaataaaac gaaaggctca 3301 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 3361 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //