##gff-version 3
##sequence-region BBa_K176226 1 3401
BBa_K176226	.	engineered_region	1	840	.	+	0	ID=BBa_K176006;Name=BBa_K176006
BBa_K176226	.	engineered_region	1	703	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K176006
BBa_K176226	.	CDS	19	678	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K176226	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K176226	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K176226	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K176226	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K176226	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176226	.	terminator	712	840	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K176006
BBa_K176226	.	terminator	800	840	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176226	.	polyA_site	827	840	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176226	.	stem_loop	807	826	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176226	.	stop_codon	833	833	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176226	.	engineered_region	800	840	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176226	.	terminator	712	791	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176226	.	stem_loop	723	766	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176226	.	engineered_region	712	791	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176226	.	engineered_region	849	3401	.	+	0	ID=BBa_K176096;Name=BBa_K176096
BBa_K176226	.	engineered_region	2765	3401	.	+	0	ID=BBa_K176063;Name=BBa_K176063;Parent=BBa_K176096
BBa_K176226	.	CDS	2785	3264	.	+	0	ID=BBa_K176003;Name=BBa_K176003;Parent=BBa_K176063
BBa_K176226	.	engineered_region	2956	3261	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176226	.	primer_binding_site	2805	2821	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K176226	.	primer_binding_site	2972	2999	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176226	.	primer_binding_site	2825	2841	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176226	.	point_mutation	3039	3039	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176226	.	engineered_region	2785	2949	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176226	.	sequence_alteration	2800	2800	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176226	.	primer_binding_site	2825	2848	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176226	.	sequence_alteration	2956	2957	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176226	.	CDS	2785	3258	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176226	.	start_codon	2785	2787	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K176226	.	point_mutation	2949	2949	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176226	.	stop_codon	3259	3264	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176226	.	terminator	3273	3352	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176063
BBa_K176226	.	stem_loop	3284	3327	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176226	.	engineered_region	3273	3352	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176226	.	terminator	3361	3401	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176063
BBa_K176226	.	polyA_site	3388	3401	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176226	.	stem_loop	3368	3387	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176226	.	stop_codon	3394	3394	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176226	.	engineered_region	3361	3401	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176226	.	ribosome_entry_site	2765	2778	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176063
BBa_K176226	.	conserved_region	2771	2774	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176226	.	engineered_region	849	2756	.	+	0	ID=BBa_K176085;Name=BBa_K176085;Parent=BBa_K176096
BBa_K176226	.	engineered_region	1698	2756	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176085
BBa_K176226	.	promoter	2685	2756	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176226	.	promoter	2704	2709	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176226	.	non_covalent_binding_site	2685	2704	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176226	.	non_covalent_binding_site	2738	2756	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176226	.	promoter	2726	2731	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176226	.	start_codon	2737	2737	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176226	.	engineered_region	2685	2737	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176226	.	engineered_region	1698	2676	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176226	.	engineered_region	1741	2676	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176226	.	CDS	1759	2514	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176226	.	CDS	1759	2508	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176226	.	point_mutation	2250	2250	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176226	.	point_mutation	1932	1932	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176226	.	engineered_tag	2515	2539	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176226	.	engineered_region	1759	2514	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176226	.	engineered_region	1759	1760	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176226	.	terminator	2636	2676	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176226	.	polyA_site	2663	2676	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176226	.	stem_loop	2643	2662	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176226	.	stop_codon	2669	2669	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176226	.	engineered_region	2636	2676	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176226	.	terminator	2548	2627	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176226	.	stem_loop	2559	2602	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176226	.	engineered_region	2548	2627	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176226	.	ribosome_entry_site	1741	1752	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176226	.	conserved_region	1745	1748	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176226	.	promoter	1698	1732	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176226	.	engineered_region	849	1689	.	+	0	ID=BBa_K176022;Name=BBa_K176022;Parent=BBa_K176085
BBa_K176226	.	promoter	849	883	.	+	0	ID=BBa_K176008;Name=BBa_K176008;Parent=BBa_K176022
BBa_K176226	.	sequence_alteration	869	869	.	+	0	ID=annotation2005562;Name=t in J23115;Parent=BBa_K176008
BBa_K176226	.	sequence_feature	866	871	.	+	0	ID=annotation2005565;Name=AvrII;Parent=BBa_K176008
BBa_K176226	.	sequence_alteration	865	865	.	+	0	ID=annotation2005561;Name=c in J23115;Parent=BBa_K176008
BBa_K176226	.	promoter	849	854	.	+	0	ID=annotation2005559;Name=-35;Parent=BBa_K176008
BBa_K176226	.	promoter	872	877	.	+	0	ID=annotation2005560;Name=-10;Parent=BBa_K176008
BBa_K176226	.	sequence_feature	878	883	.	+	0	ID=annotation2005564;Name=NheI;Parent=BBa_K176008
BBa_K176226	.	sequence_feature	855	860	.	+	0	ID=annotation2005563;Name=NheI;Parent=BBa_K176008
BBa_K176226	.	engineered_region	892	1689	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176022
BBa_K176226	.	engineered_region	892	1552	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176226	.	CDS	910	1527	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176226	.	CDS	910	1488	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176226	.	tag	1489	1520	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176226	.	engineered_tag	1528	1552	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176226	.	engineered_region	910	1527	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176226	.	ribosome_entry_site	892	903	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176226	.	conserved_region	896	899	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176226	.	terminator	1561	1689	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176226	.	terminator	1649	1689	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176226	.	polyA_site	1676	1689	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176226	.	stem_loop	1656	1675	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176226	.	stop_codon	1682	1682	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176226	.	engineered_region	1649	1689	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176226	.	terminator	1561	1640	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176226	.	stem_loop	1572	1615	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176226	.	engineered_region	1561	1640	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K176226
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttatagctagctcagtccta
ggtacaatgctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaatt
ccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtag
aaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaag
tggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagt
gctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgcta
gtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaac
atcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtatta
ggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacg
aaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactag
agaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtag
aagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatt
tatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacg
ctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaa
caatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccct
attcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttt
tacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataa
atcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttca
aaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgct
gccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgcta
gtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagac
ctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagt
cacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccct
ggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatc
gcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtct
gtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctg
tcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatgg
ccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaa
cctgatgttctggggaatataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tata