LOCUS BBa_K176229 4070 bp DNA linear 22-Nov-2024 DEFINITION High input score automatic selector: PoPS->luxI-LVA->K176226 ACCESSION BBa_K176229 VERSION BBa_K176229.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 598..629 /label=LVA CDS 19..597 /label=luxI misc_feature 19..636 /label=BBa_C0061 misc_feature 637..661 /label=Help:Barcodes CDS 19..636 /label=BBa_C0061 misc_feature 1..661 /label=BBa_C0261 misc_feature 1565..1568 /label=conserved RBS 1561..1572 /label=BBa_B0034 misc_feature 2158..2189 /label=LVA CDS 1579..2157 /label=luxI misc_feature 1579..2196 /label=BBa_C0061 misc_feature 2197..2221 /label=Help:Barcodes CDS 1579..2196 /label=BBa_C0061 misc_feature 1561..2221 /label=BBa_C0261 misc_feature 674..677 /label=conserved RBS 670..681 /label=BBa_B0034 CDS 691..1307 /label=tetR misc_feature 1308..1341 /label=SsrA misc_feature 1348..1372 /label=Help:Barcodes CDS 688..1347 /label=BBa_C0040 misc_feature 670..1372 /label=BBa_S0101 stem_loop 1392..1435 /label=stem_loop misc_feature 1381..1460 /label=BBa_B0010 terminator 1381..1460 /label=BBa_B0010 stem_loop 1476..1495 /label=T7 TE misc_feature 1502..1502 /label=stop polyA_site 1496..1509 /label=polya misc_feature 1469..1509 /label=BBa_B0012 terminator 1469..1509 /label=BBa_B0012 terminator 1381..1509 /label=BBa_B0015 misc_feature 670..1509 /label=BBa_K176006 promoter 1518..1523 /label=-35 promoter 1541..1546 /label=-10 misc_feature 1534..1534 /label=c in J23115 misc_feature 1538..1538 /label=t in J23115 misc_feature 1524..1529 /label=NheI misc_feature 1547..1552 /label=NheI misc_feature 1535..1540 /label=AvrII promoter 1518..1552 /label=BBa_K176008 misc_feature 1565..1568 /label=conserved RBS 1561..1572 /label=BBa_B0034 misc_feature 2158..2189 /label=LVA CDS 1579..2157 /label=luxI misc_feature 1579..2196 /label=BBa_C0061 misc_feature 2197..2221 /label=Help:Barcodes CDS 1579..2196 /label=BBa_C0061 misc_feature 1561..2221 /label=BBa_C0261 stem_loop 2241..2284 /label=stem_loop misc_feature 2230..2309 /label=BBa_B0010 terminator 2230..2309 /label=BBa_B0010 stem_loop 2325..2344 /label=T7 TE misc_feature 2351..2351 /label=stop polyA_site 2345..2358 /label=polya misc_feature 2318..2358 /label=BBa_B0012 terminator 2318..2358 /label=BBa_B0012 terminator 2230..2358 /label=BBa_B0015 misc_feature 1561..2358 /label=BBa_K082014 misc_feature 1518..2358 /label=BBa_K176022 promoter 2367..2401 /label=BBa_J23101 misc_feature 2414..2417 /label=conserved RBS 2410..2421 /label=BBa_B0034 misc_feature 2428..2429 /label=prefix misc_feature 2601..2601 /label=T misc_feature 2919..2919 /label=A CDS 2428..3177 /label=luxR misc_feature 2428..3183 /label=BBa_C0062 misc_feature 3184..3208 /label=Help:Barcodes CDS 2428..3183 /label=BBa_C0062 stem_loop 3228..3271 /label=stem_loop misc_feature 3217..3296 /label=BBa_B0010 terminator 3217..3296 /label=BBa_B0010 stem_loop 3312..3331 /label=T7 TE misc_feature 3338..3338 /label=stop polyA_site 3332..3345 /label=polya misc_feature 3305..3345 /label=BBa_B0012 terminator 3305..3345 /label=BBa_B0012 misc_feature 2410..3345 /label=BBa_I0462 misc_feature 2367..3345 /label=BBa_K176025 misc_feature 3354..3406 /label=R0062 promoter 3373..3378 /label=-35 promoter 3395..3400 /label=-10 misc_feature 3407..3425 /label=tetR misc_feature 3406..3406 /label=+1 misc_feature 3354..3373 /label=luxR/HSL promoter 3354..3425 /label=BBa_K176000 misc_feature 2367..3425 /label=BBa_K176028 misc_feature 1518..3425 /label=BBa_K176085 misc_feature 3440..3443 /label=conserved RBS 3434..3447 /label=BBa_B0031 misc_feature 3454..3618 /label=a part of lacZalpha (I732006) misc_feature 3454..3456 /label=Start misc_feature 3469..3469 /label=177bp and a in J32026 primer_bind 3474..3490 /label=M13 -20 primer primer_bind 3494..3517 /label=M13 -47 primer (K176059) primer_bind 3494..3510 /label=M13 -40 primer misc_feature 3618..3618 /label=g and 69bp in I732006 misc_feature 3625..3626 /label=at in K145151 misc_feature 3625..3930 /label=ccdB (K145151) misc_feature 3708..3708 /label=c in K145151 misc_feature 3928..3933 /label=Stop primer_bind 3641..3668 /label=VRccdB primer (K176058) CDS 3454..3927 /label=lacZalpha-ccdB CDS 3454..3933 /label=BBa_K176003 stem_loop 3953..3996 /label=stem_loop misc_feature 3942..4021 /label=BBa_B0010 terminator 3942..4021 /label=BBa_B0010 stem_loop 4037..4056 /label=T7 TE misc_feature 4063..4063 /label=stop polyA_site 4057..4070 /label=polya misc_feature 4030..4070 /label=BBa_B0012 terminator 4030..4070 /label=BBa_B0012 misc_feature 3434..4070 /label=BBa_K176063 misc_feature 1518..4070 /label=BBa_K176096 misc_feature 670..4070 /label=BBa_K176226 ORIGIN 1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 661 ctactagaga aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac 721 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc 781 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg 841 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa 901 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta 961 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa 1021 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta 1081 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat 1141 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga 1201 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa 1261 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac 1321 gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag 1381 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1441 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1501 gcgtttatat actagagttt atagctagct cagtcctagg tacaatgcta gctactagag 1561 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 1621 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 1681 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 1741 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 1801 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 1861 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 1921 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 1981 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 2041 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 2101 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 2161 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 2221 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2281 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2341 ggcctttctg cgtttatata ctagagttta cagctagctc agtcctaggt attatgctag 2401 ctactagaga aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga 2461 ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat 2521 atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg 2581 gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca atattatgat 2641 gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca 2701 attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa 2761 gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat 2821 ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt 2881 ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa 2941 ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta 3001 gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag 3061 cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc 3121 caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa 3181 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 3241 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 3301 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacctgta 3361 ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaatatccc tatcagtgat 3421 agagatacta gagtcacaca ggaaacctac tagatgacca tgattacgga ttcactggcc 3481 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 3541 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 3601 caacagttgc gcagcctata cgtacggcag tttaaggttt acacctataa aagagagagc 3661 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg 3721 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg 3781 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg 3841 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac 3901 gccattaacc tgatgttctg gggaatataa taatactaga gccaggcatc aaataaaacg 3961 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 4021 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //