##gff-version 3
##sequence-region BBa_K176229 1 4070
BBa_K176229	.	engineered_region	670	4070	.	+	0	ID=BBa_K176226;Name=BBa_K176226
BBa_K176229	.	engineered_region	1518	4070	.	+	0	ID=BBa_K176096;Name=BBa_K176096;Parent=BBa_K176226
BBa_K176229	.	engineered_region	3434	4070	.	+	0	ID=BBa_K176063;Name=BBa_K176063;Parent=BBa_K176096
BBa_K176229	.	terminator	3942	4021	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176063
BBa_K176229	.	stem_loop	3953	3996	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176229	.	engineered_region	3942	4021	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176229	.	CDS	3454	3933	.	+	0	ID=BBa_K176003;Name=BBa_K176003;Parent=BBa_K176063
BBa_K176229	.	primer_binding_site	3494	3517	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176229	.	engineered_region	3454	3618	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176229	.	engineered_region	3625	3930	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176229	.	point_mutation	3618	3618	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176229	.	point_mutation	3708	3708	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176229	.	sequence_alteration	3625	3626	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176229	.	sequence_alteration	3469	3469	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176229	.	start_codon	3454	3456	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K176229	.	primer_binding_site	3494	3510	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176229	.	stop_codon	3928	3933	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176229	.	primer_binding_site	3474	3490	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K176229	.	CDS	3454	3927	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176229	.	primer_binding_site	3641	3668	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176229	.	terminator	4030	4070	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176063
BBa_K176229	.	polyA_site	4057	4070	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176229	.	stem_loop	4037	4056	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176229	.	stop_codon	4063	4063	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176229	.	engineered_region	4030	4070	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176229	.	ribosome_entry_site	3434	3447	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176063
BBa_K176229	.	conserved_region	3440	3443	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176229	.	engineered_region	1518	3425	.	+	0	ID=BBa_K176085;Name=BBa_K176085;Parent=BBa_K176096
BBa_K176229	.	engineered_region	2367	3425	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176085
BBa_K176229	.	engineered_region	2367	3345	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176229	.	promoter	2367	2401	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176229	.	engineered_region	2410	3345	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176229	.	terminator	3217	3296	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176229	.	stem_loop	3228	3271	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176229	.	engineered_region	3217	3296	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176229	.	terminator	3305	3345	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176229	.	polyA_site	3332	3345	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176229	.	stem_loop	3312	3331	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176229	.	stop_codon	3338	3338	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176229	.	engineered_region	3305	3345	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176229	.	ribosome_entry_site	2410	2421	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176229	.	conserved_region	2414	2417	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176229	.	CDS	2428	3183	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176229	.	CDS	2428	3177	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176229	.	point_mutation	2919	2919	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176229	.	engineered_tag	3184	3208	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176229	.	point_mutation	2601	2601	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176229	.	engineered_region	2428	2429	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176229	.	engineered_region	2428	3183	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176229	.	promoter	3354	3425	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176229	.	promoter	3373	3378	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176229	.	non_covalent_binding_site	3354	3373	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176229	.	non_covalent_binding_site	3407	3425	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176229	.	start_codon	3406	3406	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176229	.	promoter	3395	3400	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176229	.	engineered_region	3354	3406	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176229	.	engineered_region	1518	2358	.	+	0	ID=BBa_K176022;Name=BBa_K176022;Parent=BBa_K176085
BBa_K176229	.	promoter	1518	1552	.	+	0	ID=BBa_K176008;Name=BBa_K176008;Parent=BBa_K176022
BBa_K176229	.	sequence_alteration	1538	1538	.	+	0	ID=annotation2005562;Name=t in J23115;Parent=BBa_K176008
BBa_K176229	.	sequence_feature	1535	1540	.	+	0	ID=annotation2005565;Name=AvrII;Parent=BBa_K176008
BBa_K176229	.	sequence_alteration	1534	1534	.	+	0	ID=annotation2005561;Name=c in J23115;Parent=BBa_K176008
BBa_K176229	.	promoter	1518	1523	.	+	0	ID=annotation2005559;Name=-35;Parent=BBa_K176008
BBa_K176229	.	promoter	1541	1546	.	+	0	ID=annotation2005560;Name=-10;Parent=BBa_K176008
BBa_K176229	.	sequence_feature	1547	1552	.	+	0	ID=annotation2005564;Name=NheI;Parent=BBa_K176008
BBa_K176229	.	sequence_feature	1524	1529	.	+	0	ID=annotation2005563;Name=NheI;Parent=BBa_K176008
BBa_K176229	.	engineered_region	1561	2358	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176022
BBa_K176229	.	engineered_region	1561	2221	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176229	.	CDS	1579	2196	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176229	.	CDS	1579	2157	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176229	.	engineered_region	1579	2196	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176229	.	engineered_tag	2197	2221	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176229	.	tag	2158	2189	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176229	.	ribosome_entry_site	1561	1572	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176229	.	conserved_region	1565	1568	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176229	.	terminator	2230	2358	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176229	.	terminator	2318	2358	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176229	.	polyA_site	2345	2358	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176229	.	stem_loop	2325	2344	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176229	.	stop_codon	2351	2351	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176229	.	engineered_region	2318	2358	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176229	.	terminator	2230	2309	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176229	.	stem_loop	2241	2284	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176229	.	engineered_region	2230	2309	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176229	.	engineered_region	670	1509	.	+	0	ID=BBa_K176006;Name=BBa_K176006;Parent=BBa_K176226
BBa_K176229	.	terminator	1381	1509	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K176006
BBa_K176229	.	terminator	1469	1509	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176229	.	polyA_site	1496	1509	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176229	.	stem_loop	1476	1495	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176229	.	stop_codon	1502	1502	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176229	.	engineered_region	1469	1509	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176229	.	terminator	1381	1460	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176229	.	stem_loop	1392	1435	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176229	.	engineered_region	1381	1460	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176229	.	engineered_region	670	1372	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K176006
BBa_K176229	.	ribosome_entry_site	670	681	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K176229	.	conserved_region	674	677	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176229	.	CDS	688	1347	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K176229	.	engineered_tag	1348	1372	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K176229	.	tag	1308	1341	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K176229	.	CDS	691	1307	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K176229	.	engineered_region	1561	2221	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K176229	.	CDS	1579	2196	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176229	.	CDS	1579	2157	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176229	.	engineered_region	1579	2196	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176229	.	engineered_tag	2197	2221	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176229	.	tag	2158	2189	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176229	.	ribosome_entry_site	1561	1572	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176229	.	conserved_region	1565	1568	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176229	.	engineered_region	1	661	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K176229	.	CDS	19	636	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176229	.	CDS	19	597	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176229	.	engineered_region	19	636	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176229	.	engineered_tag	637	661	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176229	.	tag	598	629	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176229	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176229	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K176229
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgtccagattagataaaagtaaa
gtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctag
gtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttaga
taggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttaga
tgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcg
aaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattt
tactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatg
ccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattga
tcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagctta
ataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtttatagctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagat
gactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaa
gtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcag
aatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgct
gaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgct
gtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacg
ctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttcc
ttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaa
cagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagt
gtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttta
cagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacg
acacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaa
aatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagat
aattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaact
ccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagc
gaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgca
cattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctag
ttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagc
gtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccattta
accaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgatt
gcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttat
agtcgaatatccctatcagtgatagagatactagagtcacacaggaaacctactagatgaccatgattacggattcact
ggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttc
gccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagttta
aggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcg
acggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatc
ggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatc
tcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata