LOCUS BBa_K176230 4816 bp DNA linear 22-Dec-2024 DEFINITION High input score automatic selector (with reporter): PoPS->GFP->K176229 ACCESSION BBa_K176230 VERSION BBa_K176230.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..738 /label=GFP protein CDS 19..738 /label=BBa_E0040 misc_feature 1..738 /label=BBa_I13500 misc_feature 751..754 /label=conserved RBS 747..758 /label=BBa_B0034 misc_feature 1344..1375 /label=LVA CDS 765..1343 /label=luxI misc_feature 765..1382 /label=BBa_C0061 misc_feature 1383..1407 /label=Help:Barcodes CDS 765..1382 /label=BBa_C0061 misc_feature 747..1407 /label=BBa_C0261 misc_feature 1..1407 /label=BBa_K176106 misc_feature 1420..1423 /label=conserved RBS 1416..1427 /label=BBa_B0034 CDS 1437..2053 /label=tetR misc_feature 2054..2087 /label=SsrA misc_feature 2094..2118 /label=Help:Barcodes CDS 1434..2093 /label=BBa_C0040 misc_feature 1416..2118 /label=BBa_S0101 stem_loop 2138..2181 /label=stem_loop misc_feature 2127..2206 /label=BBa_B0010 terminator 2127..2206 /label=BBa_B0010 stem_loop 2222..2241 /label=T7 TE misc_feature 2248..2248 /label=stop polyA_site 2242..2255 /label=polya misc_feature 2215..2255 /label=BBa_B0012 terminator 2215..2255 /label=BBa_B0012 terminator 2127..2255 /label=BBa_B0015 misc_feature 1416..2255 /label=BBa_K176006 promoter 2264..2269 /label=-35 promoter 2287..2292 /label=-10 misc_feature 2280..2280 /label=c in J23115 misc_feature 2284..2284 /label=t in J23115 misc_feature 2270..2275 /label=NheI misc_feature 2293..2298 /label=NheI misc_feature 2281..2286 /label=AvrII promoter 2264..2298 /label=BBa_K176008 misc_feature 2311..2314 /label=conserved RBS 2307..2318 /label=BBa_B0034 misc_feature 2904..2935 /label=LVA CDS 2325..2903 /label=luxI misc_feature 2325..2942 /label=BBa_C0061 misc_feature 2943..2967 /label=Help:Barcodes CDS 2325..2942 /label=BBa_C0061 misc_feature 2307..2967 /label=BBa_C0261 stem_loop 2987..3030 /label=stem_loop misc_feature 2976..3055 /label=BBa_B0010 terminator 2976..3055 /label=BBa_B0010 stem_loop 3071..3090 /label=T7 TE misc_feature 3097..3097 /label=stop polyA_site 3091..3104 /label=polya misc_feature 3064..3104 /label=BBa_B0012 terminator 3064..3104 /label=BBa_B0012 terminator 2976..3104 /label=BBa_B0015 misc_feature 2307..3104 /label=BBa_K082014 misc_feature 2264..3104 /label=BBa_K176022 promoter 3113..3147 /label=BBa_J23101 misc_feature 3160..3163 /label=conserved RBS 3156..3167 /label=BBa_B0034 misc_feature 3174..3175 /label=prefix misc_feature 3347..3347 /label=T misc_feature 3665..3665 /label=A CDS 3174..3923 /label=luxR misc_feature 3174..3929 /label=BBa_C0062 misc_feature 3930..3954 /label=Help:Barcodes CDS 3174..3929 /label=BBa_C0062 stem_loop 3974..4017 /label=stem_loop misc_feature 3963..4042 /label=BBa_B0010 terminator 3963..4042 /label=BBa_B0010 stem_loop 4058..4077 /label=T7 TE misc_feature 4084..4084 /label=stop polyA_site 4078..4091 /label=polya misc_feature 4051..4091 /label=BBa_B0012 terminator 4051..4091 /label=BBa_B0012 misc_feature 3156..4091 /label=BBa_I0462 misc_feature 3113..4091 /label=BBa_K176025 misc_feature 4100..4152 /label=R0062 promoter 4119..4124 /label=-35 promoter 4141..4146 /label=-10 misc_feature 4153..4171 /label=tetR misc_feature 4152..4152 /label=+1 misc_feature 4100..4119 /label=luxR/HSL promoter 4100..4171 /label=BBa_K176000 misc_feature 3113..4171 /label=BBa_K176028 misc_feature 2264..4171 /label=BBa_K176085 misc_feature 4186..4189 /label=conserved RBS 4180..4193 /label=BBa_B0031 misc_feature 4200..4364 /label=a part of lacZalpha (I732006) misc_feature 4200..4202 /label=Start misc_feature 4215..4215 /label=177bp and a in J32026 primer_bind 4220..4236 /label=M13 -20 primer primer_bind 4240..4263 /label=M13 -47 primer (K176059) primer_bind 4240..4256 /label=M13 -40 primer misc_feature 4364..4364 /label=g and 69bp in I732006 misc_feature 4371..4372 /label=at in K145151 misc_feature 4371..4676 /label=ccdB (K145151) misc_feature 4454..4454 /label=c in K145151 misc_feature 4674..4679 /label=Stop primer_bind 4387..4414 /label=VRccdB primer (K176058) CDS 4200..4673 /label=lacZalpha-ccdB CDS 4200..4679 /label=BBa_K176003 stem_loop 4699..4742 /label=stem_loop misc_feature 4688..4767 /label=BBa_B0010 terminator 4688..4767 /label=BBa_B0010 stem_loop 4783..4802 /label=T7 TE misc_feature 4809..4809 /label=stop polyA_site 4803..4816 /label=polya misc_feature 4776..4816 /label=BBa_B0012 terminator 4776..4816 /label=BBa_B0012 misc_feature 4180..4816 /label=BBa_K176063 misc_feature 2264..4816 /label=BBa_K176096 misc_feature 1416..4816 /label=BBa_K176226 ORIGIN 1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 721 gaactataca aataataata ctagagaaag aggagaaata ctagatgact ataatgataa 781 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 841 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 901 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 961 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 1021 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 1081 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 1141 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 1201 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 1261 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 1321 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 1381 aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac tagatgtcca 1441 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg 1501 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt 1561 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc 1621 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg 1681 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta 1741 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac 1801 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag 1861 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta 1921 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag 1981 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat 2041 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 2101 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2161 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2221 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtttatag ctagctcagt 2281 cctaggtaca atgctagcta ctagagaaag aggagaaata ctagatgact ataatgataa 2341 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 2401 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 2461 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 2521 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 2581 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 2641 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 2701 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 2761 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 2821 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 2881 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 2941 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 3001 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 3061 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtttacagc 3121 tagctcagtc ctaggtatta tgctagctac tagagaaaga ggagaaatac tagatgaaaa 3181 acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata 3241 atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac 3301 tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta gataattacc 3361 ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag 3421 attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa 3481 ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta 3541 gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa 3601 aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc 3661 cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt 3721 taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata 3781 tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa 3841 tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa 3901 ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga tcactactag 3961 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 4021 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 4081 ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt 4141 tatagtcgaa tatccctatc agtgatagag atactagagt cacacaggaa acctactaga 4201 tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 4261 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 4321 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctatacgta cggcagttta 4381 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata 4441 ttattgacac gccggggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag 4501 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga 4561 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca 4621 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataataat 4681 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 4741 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 4801 cctttctgcg tttata //