##gff-version 3
##sequence-region BBa_K176231 1 4887
BBa_K176231	.	engineered_region	72	4887	.	+	0	ID=BBa_K176230;Name=BBa_K176230
BBa_K176231	.	engineered_region	1487	4887	.	+	0	ID=BBa_K176226;Name=BBa_K176226;Parent=BBa_K176230
BBa_K176231	.	engineered_region	2335	4887	.	+	0	ID=BBa_K176096;Name=BBa_K176096;Parent=BBa_K176226
BBa_K176231	.	engineered_region	4251	4887	.	+	0	ID=BBa_K176063;Name=BBa_K176063;Parent=BBa_K176096
BBa_K176231	.	terminator	4759	4838	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176063
BBa_K176231	.	stem_loop	4770	4813	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176231	.	engineered_region	4759	4838	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176231	.	CDS	4271	4750	.	+	0	ID=BBa_K176003;Name=BBa_K176003;Parent=BBa_K176063
BBa_K176231	.	point_mutation	4435	4435	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176231	.	stop_codon	4745	4750	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176231	.	sequence_alteration	4442	4443	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176231	.	primer_binding_site	4291	4307	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K176231	.	engineered_region	4271	4435	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176231	.	CDS	4271	4744	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176231	.	start_codon	4271	4273	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K176231	.	point_mutation	4525	4525	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176231	.	primer_binding_site	4458	4485	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176231	.	sequence_alteration	4286	4286	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176231	.	engineered_region	4442	4747	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176231	.	primer_binding_site	4311	4334	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176231	.	primer_binding_site	4311	4327	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176231	.	terminator	4847	4887	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176063
BBa_K176231	.	engineered_region	4847	4887	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176231	.	stop_codon	4880	4880	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176231	.	stem_loop	4854	4873	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176231	.	polyA_site	4874	4887	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176231	.	ribosome_entry_site	4251	4264	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176063
BBa_K176231	.	conserved_region	4257	4260	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176231	.	engineered_region	2335	4242	.	+	0	ID=BBa_K176085;Name=BBa_K176085;Parent=BBa_K176096
BBa_K176231	.	engineered_region	3184	4242	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176085
BBa_K176231	.	engineered_region	3184	4162	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176231	.	engineered_region	3227	4162	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176231	.	terminator	4034	4113	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176231	.	stem_loop	4045	4088	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176231	.	engineered_region	4034	4113	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176231	.	ribosome_entry_site	3227	3238	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176231	.	conserved_region	3231	3234	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176231	.	CDS	3245	4000	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176231	.	CDS	3245	3994	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176231	.	engineered_region	3245	4000	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176231	.	engineered_tag	4001	4025	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176231	.	point_mutation	3418	3418	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176231	.	engineered_region	3245	3246	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176231	.	point_mutation	3736	3736	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176231	.	terminator	4122	4162	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176231	.	engineered_region	4122	4162	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176231	.	stop_codon	4155	4155	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176231	.	stem_loop	4129	4148	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176231	.	polyA_site	4149	4162	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176231	.	promoter	3184	3218	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176231	.	promoter	4171	4242	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176231	.	non_covalent_binding_site	4224	4242	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176231	.	non_covalent_binding_site	4171	4190	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176231	.	promoter	4190	4195	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176231	.	promoter	4212	4217	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176231	.	start_codon	4223	4223	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176231	.	engineered_region	4171	4223	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176231	.	engineered_region	2335	3175	.	+	0	ID=BBa_K176022;Name=BBa_K176022;Parent=BBa_K176085
BBa_K176231	.	promoter	2335	2369	.	+	0	ID=BBa_K176008;Name=BBa_K176008;Parent=BBa_K176022
BBa_K176231	.	sequence_alteration	2351	2351	.	+	0	ID=annotation2005561;Name=c in J23115;Parent=BBa_K176008
BBa_K176231	.	sequence_feature	2341	2346	.	+	0	ID=annotation2005563;Name=NheI;Parent=BBa_K176008
BBa_K176231	.	sequence_feature	2364	2369	.	+	0	ID=annotation2005564;Name=NheI;Parent=BBa_K176008
BBa_K176231	.	sequence_feature	2352	2357	.	+	0	ID=annotation2005565;Name=AvrII;Parent=BBa_K176008
BBa_K176231	.	sequence_alteration	2355	2355	.	+	0	ID=annotation2005562;Name=t in J23115;Parent=BBa_K176008
BBa_K176231	.	promoter	2335	2340	.	+	0	ID=annotation2005559;Name=-35;Parent=BBa_K176008
BBa_K176231	.	promoter	2358	2363	.	+	0	ID=annotation2005560;Name=-10;Parent=BBa_K176008
BBa_K176231	.	engineered_region	2378	3175	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176022
BBa_K176231	.	terminator	3047	3175	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176231	.	terminator	3047	3126	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176231	.	stem_loop	3058	3101	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176231	.	engineered_region	3047	3126	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176231	.	terminator	3135	3175	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176231	.	engineered_region	3135	3175	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176231	.	stop_codon	3168	3168	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176231	.	stem_loop	3142	3161	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176231	.	polyA_site	3162	3175	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176231	.	engineered_region	2378	3038	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176231	.	CDS	2396	3013	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176231	.	engineered_region	2396	3013	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176231	.	tag	2975	3006	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176231	.	engineered_tag	3014	3038	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176231	.	CDS	2396	2974	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176231	.	ribosome_entry_site	2378	2389	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176231	.	conserved_region	2382	2385	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176231	.	engineered_region	1487	2326	.	+	0	ID=BBa_K176006;Name=BBa_K176006;Parent=BBa_K176226
BBa_K176231	.	engineered_region	1487	2189	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K176006
BBa_K176231	.	ribosome_entry_site	1487	1498	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K176231	.	conserved_region	1491	1494	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176231	.	CDS	1505	2164	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K176231	.	engineered_tag	2165	2189	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K176231	.	tag	2125	2158	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K176231	.	CDS	1508	2124	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K176231	.	terminator	2198	2326	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K176006
BBa_K176231	.	terminator	2198	2277	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176231	.	stem_loop	2209	2252	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176231	.	engineered_region	2198	2277	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176231	.	terminator	2286	2326	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176231	.	engineered_region	2286	2326	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176231	.	stop_codon	2319	2319	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176231	.	stem_loop	2293	2312	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176231	.	polyA_site	2313	2326	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176231	.	engineered_region	72	1478	.	+	0	ID=BBa_K176106;Name=BBa_K176106;Parent=BBa_K176230
BBa_K176231	.	engineered_region	818	1478	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K176106
BBa_K176231	.	CDS	836	1453	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176231	.	engineered_region	836	1453	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176231	.	tag	1415	1446	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176231	.	engineered_tag	1454	1478	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176231	.	CDS	836	1414	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176231	.	ribosome_entry_site	818	829	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176231	.	conserved_region	822	825	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176231	.	engineered_region	72	809	.	+	0	ID=BBa_I13500;Name=BBa_I13500;Parent=BBa_K176106
BBa_K176231	.	ribosome_entry_site	72	83	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13500
BBa_K176231	.	conserved_region	76	79	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176231	.	CDS	90	809	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13500
BBa_K176231	.	CDS	90	809	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K176231	.	promoter	1	63	.	+	0	ID=BBa_K176101;Name=BBa_K176101
BBa_K176231	.	promoter	29	63	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176101
BBa_K176231	.	sequence_feature	1	20	.	+	0	ID=BBa_K176038;Name=BBa_K176038;Parent=BBa_K176101
BBa_K176231	.	primer_binding_site	1	20	.	+	0	ID=annotation2017242;Name=VR4;Parent=BBa_K176038
>BBa_K176231
ggtttggtgcgtgttgttagtactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagagga
gaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaat
gggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcacta
ctggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccaga
tcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagat
gacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattg
attttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcaga
caaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccat
tatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgcccttt
cgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgga
tgaactatacaaataataatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttgg
caattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagt
tgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaat
gtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaac
agagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactc
tgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaaca
gtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatg
tattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaa
cgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatac
tagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaa
caacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgct
cgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttt
ttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggc
ctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatt
atatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaa
agggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagc
cagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaa
cgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagtttatagctagctcagtcctaggtacaatgctagct
actagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtat
aaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaat
cagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgttt
attacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcct
aatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatga
aactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaataga
gcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcg
gttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttag
tagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaat
actagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatat
taatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatg
gttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaat
atgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataa
aaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaac
aatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatga
acataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgattt
aaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgc
agtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttcta
aagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtaca
ggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagtcacacaggaaaccta
ctagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaactt
aatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagt
tgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtaca
gagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcc
cgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtct
ccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctgggg
aatataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata