LOCUS BBa_K176232 4887 bp DNA linear 20-Dec-2024 DEFINITION For characterization of K176230: VR7+pCon 0.36->K176230 ACCESSION BBa_K176232 VERSION BBa_K176232.1 FEATURES Location/Qualifiers primer_bind 1..20 /label=VR7 misc_feature 1..20 /label=BBa_K176041 promoter 29..34 /label=-35 misc_feature 35..40 /label=NheI misc_feature 45..45 /label=c in J23107 misc_feature 46..51 /label=AvrII promoter 52..57 /label=-10 misc_feature 58..63 /label=NheI promoter 29..63 /label=BBa_K176009 promoter 1..63 /label=BBa_K176102 misc_feature 76..79 /label=conserved RBS 72..83 /label=BBa_B0034 CDS 90..809 /label=GFP protein CDS 90..809 /label=BBa_E0040 misc_feature 72..809 /label=BBa_I13500 misc_feature 822..825 /label=conserved RBS 818..829 /label=BBa_B0034 misc_feature 1415..1446 /label=LVA CDS 836..1414 /label=luxI misc_feature 836..1453 /label=BBa_C0061 misc_feature 1454..1478 /label=Help:Barcodes CDS 836..1453 /label=BBa_C0061 misc_feature 818..1478 /label=BBa_C0261 misc_feature 72..1478 /label=BBa_K176106 misc_feature 1491..1494 /label=conserved RBS 1487..1498 /label=BBa_B0034 CDS 1508..2124 /label=tetR misc_feature 2125..2158 /label=SsrA misc_feature 2165..2189 /label=Help:Barcodes CDS 1505..2164 /label=BBa_C0040 misc_feature 1487..2189 /label=BBa_S0101 stem_loop 2209..2252 /label=stem_loop misc_feature 2198..2277 /label=BBa_B0010 terminator 2198..2277 /label=BBa_B0010 stem_loop 2293..2312 /label=T7 TE misc_feature 2319..2319 /label=stop polyA_site 2313..2326 /label=polya misc_feature 2286..2326 /label=BBa_B0012 terminator 2286..2326 /label=BBa_B0012 terminator 2198..2326 /label=BBa_B0015 misc_feature 1487..2326 /label=BBa_K176006 promoter 2335..2340 /label=-35 promoter 2358..2363 /label=-10 misc_feature 2351..2351 /label=c in J23115 misc_feature 2355..2355 /label=t in J23115 misc_feature 2341..2346 /label=NheI misc_feature 2364..2369 /label=NheI misc_feature 2352..2357 /label=AvrII promoter 2335..2369 /label=BBa_K176008 misc_feature 2382..2385 /label=conserved RBS 2378..2389 /label=BBa_B0034 misc_feature 2975..3006 /label=LVA CDS 2396..2974 /label=luxI misc_feature 2396..3013 /label=BBa_C0061 misc_feature 3014..3038 /label=Help:Barcodes CDS 2396..3013 /label=BBa_C0061 misc_feature 2378..3038 /label=BBa_C0261 stem_loop 3058..3101 /label=stem_loop misc_feature 3047..3126 /label=BBa_B0010 terminator 3047..3126 /label=BBa_B0010 stem_loop 3142..3161 /label=T7 TE misc_feature 3168..3168 /label=stop polyA_site 3162..3175 /label=polya misc_feature 3135..3175 /label=BBa_B0012 terminator 3135..3175 /label=BBa_B0012 terminator 3047..3175 /label=BBa_B0015 misc_feature 2378..3175 /label=BBa_K082014 misc_feature 2335..3175 /label=BBa_K176022 promoter 3184..3218 /label=BBa_J23101 misc_feature 3231..3234 /label=conserved RBS 3227..3238 /label=BBa_B0034 misc_feature 3245..3246 /label=prefix misc_feature 3418..3418 /label=T misc_feature 3736..3736 /label=A CDS 3245..3994 /label=luxR misc_feature 3245..4000 /label=BBa_C0062 misc_feature 4001..4025 /label=Help:Barcodes CDS 3245..4000 /label=BBa_C0062 stem_loop 4045..4088 /label=stem_loop misc_feature 4034..4113 /label=BBa_B0010 terminator 4034..4113 /label=BBa_B0010 stem_loop 4129..4148 /label=T7 TE misc_feature 4155..4155 /label=stop polyA_site 4149..4162 /label=polya misc_feature 4122..4162 /label=BBa_B0012 terminator 4122..4162 /label=BBa_B0012 misc_feature 3227..4162 /label=BBa_I0462 misc_feature 3184..4162 /label=BBa_K176025 misc_feature 4171..4223 /label=R0062 promoter 4190..4195 /label=-35 promoter 4212..4217 /label=-10 misc_feature 4224..4242 /label=tetR misc_feature 4223..4223 /label=+1 misc_feature 4171..4190 /label=luxR/HSL promoter 4171..4242 /label=BBa_K176000 misc_feature 3184..4242 /label=BBa_K176028 misc_feature 2335..4242 /label=BBa_K176085 misc_feature 4257..4260 /label=conserved RBS 4251..4264 /label=BBa_B0031 misc_feature 4271..4435 /label=a part of lacZalpha (I732006) misc_feature 4271..4273 /label=Start misc_feature 4286..4286 /label=177bp and a in J32026 primer_bind 4291..4307 /label=M13 -20 primer primer_bind 4311..4334 /label=M13 -47 primer (K176059) primer_bind 4311..4327 /label=M13 -40 primer misc_feature 4435..4435 /label=g and 69bp in I732006 misc_feature 4442..4443 /label=at in K145151 misc_feature 4442..4747 /label=ccdB (K145151) misc_feature 4525..4525 /label=c in K145151 misc_feature 4745..4750 /label=Stop primer_bind 4458..4485 /label=VRccdB primer (K176058) CDS 4271..4744 /label=lacZalpha-ccdB CDS 4271..4750 /label=BBa_K176003 stem_loop 4770..4813 /label=stem_loop misc_feature 4759..4838 /label=BBa_B0010 terminator 4759..4838 /label=BBa_B0010 stem_loop 4854..4873 /label=T7 TE misc_feature 4880..4880 /label=stop polyA_site 4874..4887 /label=polya misc_feature 4847..4887 /label=BBa_B0012 terminator 4847..4887 /label=BBa_B0012 misc_feature 4251..4887 /label=BBa_K176063 misc_feature 2335..4887 /label=BBa_K176096 misc_feature 1487..4887 /label=BBa_K176226 misc_feature 72..4887 /label=BBa_K176230 ORIGIN 1 ggtaggtcac agggcaattt tactagagtt tacggctagc tcagtcctag gtattatgct 61 agctactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag 121 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg 181 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg 241 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct 301 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag 361 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg 421 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta 481 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat 541 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca 601 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg 661 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc 721 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac 781 atggcatgga tgaactatac aaataataat actagagaaa gaggagaaat actagatgac 841 tataatgata aaaaaatcgg attttttggc aattccatcg gaggagtata aaggtattct 901 aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg gacttagttg tagaaaataa 961 ccttgaatca gatgagtatg ataactcaaa tgcagaatat atttatgctt gtgatgatac 1021 tgaaaatgta agtggatgct ggcgtttatt acctacaaca ggtgattata tgctgaaaag 1081 tgtttttcct gaattgcttg gtcaacagag tgctcccaaa gatcctaata tagtcgaatt 1141 aagtcgtttt gctgtaggta aaaatagctc aaagataaat aactctgcta gtgaaattac 1201 aatgaaacta tttgaagcta tatataaaca cgctgttagt caaggtatta cagaatatgt 1261 aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt attaaagttc cttgtcatcg 1321 tattggagac aaagaaattc atgtattagg tgatactaaa tcggttgtat tgtctatgcc 1381 tattaatgaa cagtttaaaa aagcagtctt aaatgctgca aacgacgaaa actacgcttt 1441 agtagcttaa taactctgat agtgctagtg tagatctcta ctagagaaag aggagaaata 1501 ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga 1561 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc 1621 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat 1681 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt 1741 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt 1801 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt 1861 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca 1921 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga 1981 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca 2041 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa 2101 acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta 2161 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag 2221 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 2281 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtttata 2341 gctagctcag tcctaggtac aatgctagct actagagaaa gaggagaaat actagatgac 2401 tataatgata aaaaaatcgg attttttggc aattccatcg gaggagtata aaggtattct 2461 aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg gacttagttg tagaaaataa 2521 ccttgaatca gatgagtatg ataactcaaa tgcagaatat atttatgctt gtgatgatac 2581 tgaaaatgta agtggatgct ggcgtttatt acctacaaca ggtgattata tgctgaaaag 2641 tgtttttcct gaattgcttg gtcaacagag tgctcccaaa gatcctaata tagtcgaatt 2701 aagtcgtttt gctgtaggta aaaatagctc aaagataaat aactctgcta gtgaaattac 2761 aatgaaacta tttgaagcta tatataaaca cgctgttagt caaggtatta cagaatatgt 2821 aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt attaaagttc cttgtcatcg 2881 tattggagac aaagaaattc atgtattagg tgatactaaa tcggttgtat tgtctatgcc 2941 tattaatgaa cagtttaaaa aagcagtctt aaatgctgca aacgacgaaa actacgcttt 3001 agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata 3061 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3121 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 3181 gagtttacag ctagctcagt cctaggtatt atgctagcta ctagagaaag aggagaaata 3241 ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg 3301 tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga 3361 atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct 3421 agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga 3481 tcctatagta gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa 3541 caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat 3601 cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc 3661 acattcagaa aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc 3721 attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc 3781 aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag 3841 ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac 3901 caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt 3961 aacaggagca attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag 4021 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 4081 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 4141 ggtgggcctt tctgcgttta tatactagag acctgtagga tcgtacaggt ttacgcaaga 4201 aaatggtttg ttatagtcga atatccctat cagtgataga gatactagag tcacacagga 4261 aacctactag atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg 4321 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 4381 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctatacgt 4441 acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt 4501 acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg ccagtgcacg 4561 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag 4621 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt 4681 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg 4741 aatataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 4801 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 4861 cttcgggtgg gcctttctgc gtttata //