LOCUS BBa_K176236 3472 bp DNA linear 18-Nov-2024 DEFINITION For characterization of K176226: VR7+pCon 0.36->K176226 ACCESSION BBa_K176236 VERSION BBa_K176236.1 FEATURES Location/Qualifiers primer_bind 1..20 /label=VR7 misc_feature 1..20 /label=BBa_K176041 promoter 29..34 /label=-35 misc_feature 35..40 /label=NheI misc_feature 45..45 /label=c in J23107 misc_feature 46..51 /label=AvrII promoter 52..57 /label=-10 misc_feature 58..63 /label=NheI promoter 29..63 /label=BBa_K176009 promoter 1..63 /label=BBa_K176102 misc_feature 76..79 /label=conserved RBS 72..83 /label=BBa_B0034 CDS 93..709 /label=tetR misc_feature 710..743 /label=SsrA misc_feature 750..774 /label=Help:Barcodes CDS 90..749 /label=BBa_C0040 misc_feature 72..774 /label=BBa_S0101 stem_loop 794..837 /label=stem_loop misc_feature 783..862 /label=BBa_B0010 terminator 783..862 /label=BBa_B0010 stem_loop 878..897 /label=T7 TE misc_feature 904..904 /label=stop polyA_site 898..911 /label=polya misc_feature 871..911 /label=BBa_B0012 terminator 871..911 /label=BBa_B0012 terminator 783..911 /label=BBa_B0015 misc_feature 72..911 /label=BBa_K176006 promoter 920..925 /label=-35 promoter 943..948 /label=-10 misc_feature 936..936 /label=c in J23115 misc_feature 940..940 /label=t in J23115 misc_feature 926..931 /label=NheI misc_feature 949..954 /label=NheI misc_feature 937..942 /label=AvrII promoter 920..954 /label=BBa_K176008 misc_feature 967..970 /label=conserved RBS 963..974 /label=BBa_B0034 misc_feature 1560..1591 /label=LVA CDS 981..1559 /label=luxI misc_feature 981..1598 /label=BBa_C0061 misc_feature 1599..1623 /label=Help:Barcodes CDS 981..1598 /label=BBa_C0061 misc_feature 963..1623 /label=BBa_C0261 stem_loop 1643..1686 /label=stem_loop misc_feature 1632..1711 /label=BBa_B0010 terminator 1632..1711 /label=BBa_B0010 stem_loop 1727..1746 /label=T7 TE misc_feature 1753..1753 /label=stop polyA_site 1747..1760 /label=polya misc_feature 1720..1760 /label=BBa_B0012 terminator 1720..1760 /label=BBa_B0012 terminator 1632..1760 /label=BBa_B0015 misc_feature 963..1760 /label=BBa_K082014 misc_feature 920..1760 /label=BBa_K176022 promoter 1769..1803 /label=BBa_J23101 misc_feature 1816..1819 /label=conserved RBS 1812..1823 /label=BBa_B0034 misc_feature 1830..1831 /label=prefix misc_feature 2003..2003 /label=T misc_feature 2321..2321 /label=A CDS 1830..2579 /label=luxR misc_feature 1830..2585 /label=BBa_C0062 misc_feature 2586..2610 /label=Help:Barcodes CDS 1830..2585 /label=BBa_C0062 stem_loop 2630..2673 /label=stem_loop misc_feature 2619..2698 /label=BBa_B0010 terminator 2619..2698 /label=BBa_B0010 stem_loop 2714..2733 /label=T7 TE misc_feature 2740..2740 /label=stop polyA_site 2734..2747 /label=polya misc_feature 2707..2747 /label=BBa_B0012 terminator 2707..2747 /label=BBa_B0012 misc_feature 1812..2747 /label=BBa_I0462 misc_feature 1769..2747 /label=BBa_K176025 misc_feature 2756..2808 /label=R0062 promoter 2775..2780 /label=-35 promoter 2797..2802 /label=-10 misc_feature 2809..2827 /label=tetR misc_feature 2808..2808 /label=+1 misc_feature 2756..2775 /label=luxR/HSL promoter 2756..2827 /label=BBa_K176000 misc_feature 1769..2827 /label=BBa_K176028 misc_feature 920..2827 /label=BBa_K176085 misc_feature 2842..2845 /label=conserved RBS 2836..2849 /label=BBa_B0031 misc_feature 2856..3020 /label=a part of lacZalpha (I732006) misc_feature 2856..2858 /label=Start misc_feature 2871..2871 /label=177bp and a in J32026 primer_bind 2876..2892 /label=M13 -20 primer primer_bind 2896..2919 /label=M13 -47 primer (K176059) primer_bind 2896..2912 /label=M13 -40 primer misc_feature 3020..3020 /label=g and 69bp in I732006 misc_feature 3027..3028 /label=at in K145151 misc_feature 3027..3332 /label=ccdB (K145151) misc_feature 3110..3110 /label=c in K145151 misc_feature 3330..3335 /label=Stop primer_bind 3043..3070 /label=VRccdB primer (K176058) CDS 2856..3329 /label=lacZalpha-ccdB CDS 2856..3335 /label=BBa_K176003 stem_loop 3355..3398 /label=stem_loop misc_feature 3344..3423 /label=BBa_B0010 terminator 3344..3423 /label=BBa_B0010 stem_loop 3439..3458 /label=T7 TE misc_feature 3465..3465 /label=stop polyA_site 3459..3472 /label=polya misc_feature 3432..3472 /label=BBa_B0012 terminator 3432..3472 /label=BBa_B0012 misc_feature 2836..3472 /label=BBa_K176063 misc_feature 920..3472 /label=BBa_K176096 misc_feature 72..3472 /label=BBa_K176226 ORIGIN 1 ggtaggtcac agggcaattt tactagagtt tacggctagc tcagtcctag gtattatgct 61 agctactaga gaaagaggag aaatactaga tgtccagatt agataaaagt aaagtgatta 121 acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg 181 cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt 241 tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag 301 aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac 361 taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg 421 aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat 481 tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc 541 atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac 601 gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg 661 aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg 721 acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag 781 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 841 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 901 ctgcgtttat atactagagt ttatagctag ctcagtccta ggtacaatgc tagctactag 961 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc 1021 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg 1081 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag 1141 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta 1201 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc 1261 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga 1321 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg 1381 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa 1441 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata 1501 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg 1561 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat 1621 ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1681 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 1741 tgggcctttc tgcgtttata tactagagtt tacagctagc tcagtcctag gtattatgct 1801 agctactaga gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca 1861 gaataattaa taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg 1921 atatgactaa aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta 1981 tggttaaatc tgatatttca atcctagata attaccctaa aaaatggagg caatattatg 2041 atgacgctaa tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac 2101 caattaattg gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta 2161 aagaagcgaa aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca 2221 atggcttcgg aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat 2281 ttttacatgc gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa 2341 aaataaatat agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt 2401 tagcgtgggc atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg 2461 agcgtactgt cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct 2521 gccaaagtat ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt 2581 aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa 2641 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 2701 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg 2761 taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaatatc cctatcagtg 2821 atagagatac tagagtcaca caggaaacct actagatgac catgattacg gattcactgg 2881 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 2941 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 3001 cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat aaaagagaga 3061 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg gggcgacgga 3121 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc 3181 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc 3241 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa 3301 acgccattaa cctgatgttc tggggaatat aataatacta gagccaggca tcaaataaaa 3361 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 3421 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //