##gff-version 3
##sequence-region BBa_K176236 1 3472
BBa_K176236	.	promoter	1	63	.	+	0	ID=BBa_K176102;Name=BBa_K176102
BBa_K176236	.	sequence_feature	1	20	.	+	0	ID=BBa_K176041;Name=BBa_K176041;Parent=BBa_K176102
BBa_K176236	.	primer_binding_site	1	20	.	+	0	ID=annotation2017245;Name=VR7;Parent=BBa_K176041
BBa_K176236	.	promoter	29	63	.	+	0	ID=BBa_K176009;Name=BBa_K176009;Parent=BBa_K176102
BBa_K176236	.	sequence_feature	58	63	.	+	0	ID=annotation2005860;Name=NheI;Parent=BBa_K176009
BBa_K176236	.	sequence_alteration	45	45	.	+	0	ID=annotation2005857;Name=c in J23107;Parent=BBa_K176009
BBa_K176236	.	promoter	29	34	.	+	0	ID=annotation2005855;Name=-35;Parent=BBa_K176009
BBa_K176236	.	sequence_feature	46	51	.	+	0	ID=annotation2005858;Name=AvrII;Parent=BBa_K176009
BBa_K176236	.	promoter	52	57	.	+	0	ID=annotation2005859;Name=-10;Parent=BBa_K176009
BBa_K176236	.	sequence_feature	35	40	.	+	0	ID=annotation2005856;Name=NheI;Parent=BBa_K176009
BBa_K176236	.	engineered_region	72	3472	.	+	0	ID=BBa_K176226;Name=BBa_K176226
BBa_K176236	.	engineered_region	920	3472	.	+	0	ID=BBa_K176096;Name=BBa_K176096;Parent=BBa_K176226
BBa_K176236	.	engineered_region	2836	3472	.	+	0	ID=BBa_K176063;Name=BBa_K176063;Parent=BBa_K176096
BBa_K176236	.	terminator	3432	3472	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176063
BBa_K176236	.	polyA_site	3459	3472	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176236	.	stop_codon	3465	3465	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176236	.	stem_loop	3439	3458	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176236	.	engineered_region	3432	3472	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176236	.	ribosome_entry_site	2836	2849	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176063
BBa_K176236	.	conserved_region	2842	2845	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176236	.	terminator	3344	3423	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176063
BBa_K176236	.	stem_loop	3355	3398	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176236	.	engineered_region	3344	3423	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176236	.	CDS	2856	3335	.	+	0	ID=BBa_K176003;Name=BBa_K176003;Parent=BBa_K176063
BBa_K176236	.	point_mutation	3020	3020	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176236	.	stop_codon	3330	3335	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176236	.	point_mutation	3110	3110	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176236	.	primer_binding_site	2896	2919	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176236	.	primer_binding_site	2896	2912	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176236	.	engineered_region	2856	3020	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176236	.	start_codon	2856	2858	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K176236	.	sequence_alteration	3027	3028	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176236	.	primer_binding_site	2876	2892	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K176236	.	engineered_region	3027	3332	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176236	.	sequence_alteration	2871	2871	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176236	.	primer_binding_site	3043	3070	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176236	.	CDS	2856	3329	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176236	.	engineered_region	920	2827	.	+	0	ID=BBa_K176085;Name=BBa_K176085;Parent=BBa_K176096
BBa_K176236	.	engineered_region	920	1760	.	+	0	ID=BBa_K176022;Name=BBa_K176022;Parent=BBa_K176085
BBa_K176236	.	engineered_region	963	1760	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176022
BBa_K176236	.	terminator	1632	1760	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176236	.	terminator	1720	1760	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176236	.	polyA_site	1747	1760	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176236	.	stop_codon	1753	1753	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176236	.	stem_loop	1727	1746	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176236	.	engineered_region	1720	1760	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176236	.	terminator	1632	1711	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176236	.	stem_loop	1643	1686	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176236	.	engineered_region	1632	1711	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176236	.	engineered_region	963	1623	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176236	.	ribosome_entry_site	963	974	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176236	.	conserved_region	967	970	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176236	.	CDS	981	1598	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176236	.	tag	1560	1591	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176236	.	engineered_region	981	1598	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176236	.	engineered_tag	1599	1623	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176236	.	CDS	981	1559	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176236	.	promoter	920	954	.	+	0	ID=BBa_K176008;Name=BBa_K176008;Parent=BBa_K176022
BBa_K176236	.	sequence_feature	937	942	.	+	0	ID=annotation2005565;Name=AvrII;Parent=BBa_K176008
BBa_K176236	.	promoter	920	925	.	+	0	ID=annotation2005559;Name=-35;Parent=BBa_K176008
BBa_K176236	.	sequence_feature	926	931	.	+	0	ID=annotation2005563;Name=NheI;Parent=BBa_K176008
BBa_K176236	.	sequence_feature	949	954	.	+	0	ID=annotation2005564;Name=NheI;Parent=BBa_K176008
BBa_K176236	.	sequence_alteration	936	936	.	+	0	ID=annotation2005561;Name=c in J23115;Parent=BBa_K176008
BBa_K176236	.	sequence_alteration	940	940	.	+	0	ID=annotation2005562;Name=t in J23115;Parent=BBa_K176008
BBa_K176236	.	promoter	943	948	.	+	0	ID=annotation2005560;Name=-10;Parent=BBa_K176008
BBa_K176236	.	engineered_region	1769	2827	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176085
BBa_K176236	.	engineered_region	1769	2747	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176236	.	promoter	1769	1803	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176236	.	engineered_region	1812	2747	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176236	.	terminator	2707	2747	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176236	.	polyA_site	2734	2747	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176236	.	stop_codon	2740	2740	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176236	.	stem_loop	2714	2733	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176236	.	engineered_region	2707	2747	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176236	.	CDS	1830	2585	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176236	.	engineered_region	1830	2585	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176236	.	engineered_tag	2586	2610	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176236	.	engineered_region	1830	1831	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176236	.	point_mutation	2003	2003	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176236	.	point_mutation	2321	2321	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176236	.	CDS	1830	2579	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176236	.	terminator	2619	2698	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176236	.	stem_loop	2630	2673	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176236	.	engineered_region	2619	2698	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176236	.	ribosome_entry_site	1812	1823	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176236	.	conserved_region	1816	1819	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176236	.	promoter	2756	2827	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176236	.	non_covalent_binding_site	2809	2827	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176236	.	non_covalent_binding_site	2756	2775	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176236	.	promoter	2775	2780	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176236	.	start_codon	2808	2808	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176236	.	engineered_region	2756	2808	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176236	.	promoter	2797	2802	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176236	.	engineered_region	72	911	.	+	0	ID=BBa_K176006;Name=BBa_K176006;Parent=BBa_K176226
BBa_K176236	.	terminator	783	911	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K176006
BBa_K176236	.	terminator	871	911	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176236	.	polyA_site	898	911	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176236	.	stop_codon	904	904	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176236	.	stem_loop	878	897	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176236	.	engineered_region	871	911	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176236	.	terminator	783	862	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176236	.	stem_loop	794	837	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176236	.	engineered_region	783	862	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176236	.	engineered_region	72	774	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K176006
BBa_K176236	.	CDS	90	749	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K176236	.	engineered_tag	750	774	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K176236	.	CDS	93	709	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K176236	.	tag	710	743	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K176236	.	ribosome_entry_site	72	83	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K176236	.	conserved_region	76	79	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K176236
ggtaggtcacagggcaattttactagagtttacggctagctcagtcctaggtattatgctagctactagagaaagagga
gaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaa
ggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcggg
ctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggca
agattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggt
acacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagaga
atgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaa
agaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggt
gcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccg
ctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttatagctagctcagtcctaggtacaat
gctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcgga
ggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataac
cttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgct
ggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaa
agatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaatt
acaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacag
caatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatac
taaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactac
gctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagag
gagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaata
atgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctca
ttctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaattta
ataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctg
taaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatac
ggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcg
tgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaaca
acgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatatt
aggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagt
atttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagat
cactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtagga
tcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagtcacacagg
aaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttac
ccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcc
caacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtgg
atgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataa
agtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtg
ccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgt
tctggggaatataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata