##gff-version 3
##sequence-region BBa_K176242 1 4816
BBa_K176242	.	engineered_region	1	1407	.	+	0	ID=BBa_K176106;Name=BBa_K176106
BBa_K176242	.	engineered_region	747	1407	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K176106
BBa_K176242	.	ribosome_entry_site	747	758	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176242	.	conserved_region	751	754	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176242	.	CDS	765	1382	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176242	.	tag	1344	1375	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176242	.	engineered_region	765	1382	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176242	.	engineered_tag	1383	1407	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176242	.	CDS	765	1343	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176242	.	engineered_region	1	738	.	+	0	ID=BBa_I13500;Name=BBa_I13500;Parent=BBa_K176106
BBa_K176242	.	CDS	19	738	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13500
BBa_K176242	.	CDS	19	738	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K176242	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13500
BBa_K176242	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176242	.	engineered_region	1416	4816	.	+	0	ID=BBa_K176228;Name=BBa_K176228
BBa_K176242	.	engineered_region	2264	4816	.	+	0	ID=BBa_K176095;Name=BBa_K176095;Parent=BBa_K176228
BBa_K176242	.	engineered_region	4180	4816	.	+	0	ID=BBa_K176063;Name=BBa_K176063;Parent=BBa_K176095
BBa_K176242	.	terminator	4776	4816	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176063
BBa_K176242	.	polyA_site	4803	4816	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176242	.	stop_codon	4809	4809	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176242	.	stem_loop	4783	4802	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176242	.	engineered_region	4776	4816	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176242	.	ribosome_entry_site	4180	4193	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176063
BBa_K176242	.	conserved_region	4186	4189	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176242	.	terminator	4688	4767	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176063
BBa_K176242	.	stem_loop	4699	4742	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176242	.	engineered_region	4688	4767	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176242	.	CDS	4200	4679	.	+	0	ID=BBa_K176003;Name=BBa_K176003;Parent=BBa_K176063
BBa_K176242	.	point_mutation	4364	4364	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176242	.	stop_codon	4674	4679	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176242	.	point_mutation	4454	4454	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176242	.	primer_binding_site	4240	4263	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176242	.	primer_binding_site	4240	4256	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176242	.	engineered_region	4200	4364	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176242	.	start_codon	4200	4202	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K176242	.	sequence_alteration	4371	4372	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176242	.	primer_binding_site	4220	4236	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K176242	.	engineered_region	4371	4676	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176242	.	sequence_alteration	4215	4215	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176242	.	primer_binding_site	4387	4414	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176242	.	CDS	4200	4673	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176242	.	engineered_region	2264	4171	.	+	0	ID=BBa_K176084;Name=BBa_K176084;Parent=BBa_K176095
BBa_K176242	.	engineered_region	2264	3104	.	+	0	ID=BBa_K176021;Name=BBa_K176021;Parent=BBa_K176084
BBa_K176242	.	engineered_region	2307	3104	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176021
BBa_K176242	.	terminator	2976	3104	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176242	.	terminator	3064	3104	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176242	.	polyA_site	3091	3104	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176242	.	stop_codon	3097	3097	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176242	.	stem_loop	3071	3090	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176242	.	engineered_region	3064	3104	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176242	.	terminator	2976	3055	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176242	.	stem_loop	2987	3030	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176242	.	engineered_region	2976	3055	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176242	.	engineered_region	2307	2967	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176242	.	ribosome_entry_site	2307	2318	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176242	.	conserved_region	2311	2314	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176242	.	CDS	2325	2942	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176242	.	tag	2904	2935	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176242	.	engineered_region	2325	2942	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176242	.	engineered_tag	2943	2967	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176242	.	CDS	2325	2903	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176242	.	promoter	2264	2298	.	+	0	ID=BBa_K176009;Name=BBa_K176009;Parent=BBa_K176021
BBa_K176242	.	sequence_feature	2293	2298	.	+	0	ID=annotation2005860;Name=NheI;Parent=BBa_K176009
BBa_K176242	.	sequence_alteration	2280	2280	.	+	0	ID=annotation2005857;Name=c in J23107;Parent=BBa_K176009
BBa_K176242	.	promoter	2264	2269	.	+	0	ID=annotation2005855;Name=-35;Parent=BBa_K176009
BBa_K176242	.	sequence_feature	2281	2286	.	+	0	ID=annotation2005858;Name=AvrII;Parent=BBa_K176009
BBa_K176242	.	promoter	2287	2292	.	+	0	ID=annotation2005859;Name=-10;Parent=BBa_K176009
BBa_K176242	.	sequence_feature	2270	2275	.	+	0	ID=annotation2005856;Name=NheI;Parent=BBa_K176009
BBa_K176242	.	engineered_region	3113	4171	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176084
BBa_K176242	.	engineered_region	3113	4091	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176242	.	promoter	3113	3147	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176242	.	engineered_region	3156	4091	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176242	.	terminator	4051	4091	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176242	.	polyA_site	4078	4091	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176242	.	stop_codon	4084	4084	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176242	.	stem_loop	4058	4077	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176242	.	engineered_region	4051	4091	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176242	.	CDS	3174	3929	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176242	.	engineered_region	3174	3929	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176242	.	engineered_tag	3930	3954	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176242	.	engineered_region	3174	3175	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176242	.	point_mutation	3347	3347	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176242	.	point_mutation	3665	3665	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176242	.	CDS	3174	3923	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176242	.	terminator	3963	4042	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176242	.	stem_loop	3974	4017	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176242	.	engineered_region	3963	4042	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176242	.	ribosome_entry_site	3156	3167	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176242	.	conserved_region	3160	3163	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176242	.	promoter	4100	4171	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176242	.	non_covalent_binding_site	4153	4171	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176242	.	non_covalent_binding_site	4100	4119	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176242	.	promoter	4119	4124	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176242	.	start_codon	4152	4152	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176242	.	engineered_region	4100	4152	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176242	.	promoter	4141	4146	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176242	.	engineered_region	1416	2255	.	+	0	ID=BBa_K176006;Name=BBa_K176006;Parent=BBa_K176228
BBa_K176242	.	terminator	2127	2255	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K176006
BBa_K176242	.	terminator	2215	2255	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176242	.	polyA_site	2242	2255	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176242	.	stop_codon	2248	2248	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176242	.	stem_loop	2222	2241	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176242	.	engineered_region	2215	2255	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176242	.	terminator	2127	2206	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176242	.	stem_loop	2138	2181	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176242	.	engineered_region	2127	2206	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176242	.	engineered_region	1416	2118	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K176006
BBa_K176242	.	CDS	1434	2093	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K176242	.	engineered_tag	2094	2118	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K176242	.	CDS	1437	2053	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K176242	.	tag	2054	2087	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K176242	.	ribosome_entry_site	1416	1427	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K176242	.	conserved_region	1420	1423	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K176242
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtg
atgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttat
ttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgaga
tacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttt
tcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaa
aggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatc
atggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactag
cagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatc
tgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacat
ggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatgactataatgataaaaaaatcgga
ttttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgg
gacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgata
ctgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgct
tggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagata
aataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaat
atgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaaga
aattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaat
gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagagg
agaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcga
aggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgg
gctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggc
aagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttagg
tacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagag
aatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgcta
aagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaagg
tgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcc
gctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcctaggtatta
tgctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcgg
aggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataa
ccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgc
tggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctccca
aagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaat
tacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaaca
gcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgata
ctaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaacta
cgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaaga
ggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaat
aatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctc
attctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaattt
aataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgct
gtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcata
cggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgc
gtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaac
aacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatat
taggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaag
tatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtaga
tcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtagg
atcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagtcacacag
gaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtta
cccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttc
ccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtg
gatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagata
aagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgt
gccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatg
ttctggggaatataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata