##gff-version 3
##sequence-region BBa_K176243 1 3496
BBa_K176243	.	promoter	1	63	.	+	0	ID=BBa_K176102;Name=BBa_K176102
BBa_K176243	.	sequence_feature	1	20	.	+	0	ID=BBa_K176041;Name=BBa_K176041;Parent=BBa_K176102
BBa_K176243	.	primer_binding_site	1	20	.	+	0	ID=annotation2017245;Name=VR7;Parent=BBa_K176041
BBa_K176243	.	promoter	29	63	.	+	0	ID=BBa_K176009;Name=BBa_K176009;Parent=BBa_K176102
BBa_K176243	.	promoter	52	57	.	+	0	ID=annotation2005859;Name=-10;Parent=BBa_K176009
BBa_K176243	.	sequence_feature	58	63	.	+	0	ID=annotation2005860;Name=NheI;Parent=BBa_K176009
BBa_K176243	.	promoter	29	34	.	+	0	ID=annotation2005855;Name=-35;Parent=BBa_K176009
BBa_K176243	.	sequence_feature	46	51	.	+	0	ID=annotation2005858;Name=AvrII;Parent=BBa_K176009
BBa_K176243	.	sequence_feature	35	40	.	+	0	ID=annotation2005856;Name=NheI;Parent=BBa_K176009
BBa_K176243	.	sequence_alteration	45	45	.	+	0	ID=annotation2005857;Name=c in J23107;Parent=BBa_K176009
BBa_K176243	.	engineered_region	72	3496	.	+	0	ID=BBa_K176240;Name=BBa_K176240
BBa_K176243	.	engineered_region	72	732	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K176240
BBa_K176243	.	CDS	90	707	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176243	.	engineered_region	90	707	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176243	.	engineered_tag	708	732	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176243	.	CDS	90	668	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176243	.	tag	669	700	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176243	.	ribosome_entry_site	72	83	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176243	.	conserved_region	76	79	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176243	.	engineered_region	1632	2292	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K176240
BBa_K176243	.	CDS	1650	2267	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176243	.	engineered_region	1650	2267	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176243	.	engineered_tag	2268	2292	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176243	.	CDS	1650	2228	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176243	.	tag	2229	2260	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176243	.	ribosome_entry_site	1632	1643	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176243	.	conserved_region	1636	1639	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176243	.	engineered_region	741	3496	.	+	0	ID=BBa_K176237;Name=BBa_K176237;Parent=BBa_K176240
BBa_K176243	.	engineered_region	1589	3496	.	+	0	ID=BBa_K176085;Name=BBa_K176085;Parent=BBa_K176237
BBa_K176243	.	engineered_region	1589	2429	.	+	0	ID=BBa_K176022;Name=BBa_K176022;Parent=BBa_K176085
BBa_K176243	.	promoter	1589	1623	.	+	0	ID=BBa_K176008;Name=BBa_K176008;Parent=BBa_K176022
BBa_K176243	.	sequence_feature	1595	1600	.	+	0	ID=annotation2005563;Name=NheI;Parent=BBa_K176008
BBa_K176243	.	sequence_feature	1606	1611	.	+	0	ID=annotation2005565;Name=AvrII;Parent=BBa_K176008
BBa_K176243	.	promoter	1589	1594	.	+	0	ID=annotation2005559;Name=-35;Parent=BBa_K176008
BBa_K176243	.	sequence_feature	1618	1623	.	+	0	ID=annotation2005564;Name=NheI;Parent=BBa_K176008
BBa_K176243	.	sequence_alteration	1609	1609	.	+	0	ID=annotation2005562;Name=t in J23115;Parent=BBa_K176008
BBa_K176243	.	promoter	1612	1617	.	+	0	ID=annotation2005560;Name=-10;Parent=BBa_K176008
BBa_K176243	.	sequence_alteration	1605	1605	.	+	0	ID=annotation2005561;Name=c in J23115;Parent=BBa_K176008
BBa_K176243	.	engineered_region	1632	2429	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176022
BBa_K176243	.	terminator	2301	2429	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176243	.	terminator	2389	2429	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176243	.	stem_loop	2396	2415	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176243	.	polyA_site	2416	2429	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176243	.	stop_codon	2422	2422	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176243	.	engineered_region	2389	2429	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176243	.	terminator	2301	2380	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176243	.	stem_loop	2312	2355	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176243	.	engineered_region	2301	2380	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176243	.	engineered_region	1632	2292	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176243	.	CDS	1650	2267	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176243	.	engineered_region	1650	2267	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176243	.	engineered_tag	2268	2292	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176243	.	CDS	1650	2228	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176243	.	tag	2229	2260	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176243	.	ribosome_entry_site	1632	1643	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176243	.	conserved_region	1636	1639	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176243	.	engineered_region	2438	3496	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176085
BBa_K176243	.	promoter	3425	3496	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176243	.	promoter	3444	3449	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176243	.	non_covalent_binding_site	3478	3496	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176243	.	start_codon	3477	3477	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176243	.	non_covalent_binding_site	3425	3444	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176243	.	promoter	3466	3471	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176243	.	engineered_region	3425	3477	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176243	.	engineered_region	2438	3416	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176243	.	promoter	2438	2472	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176243	.	engineered_region	2481	3416	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176243	.	CDS	2499	3254	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176243	.	engineered_region	2499	2500	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176243	.	engineered_tag	3255	3279	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176243	.	CDS	2499	3248	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176243	.	point_mutation	2990	2990	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176243	.	point_mutation	2672	2672	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176243	.	engineered_region	2499	3254	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176243	.	terminator	3376	3416	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176243	.	stem_loop	3383	3402	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176243	.	polyA_site	3403	3416	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176243	.	stop_codon	3409	3409	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176243	.	engineered_region	3376	3416	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176243	.	ribosome_entry_site	2481	2492	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176243	.	conserved_region	2485	2488	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176243	.	terminator	3288	3367	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176243	.	stem_loop	3299	3342	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176243	.	engineered_region	3288	3367	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176243	.	engineered_region	741	1580	.	+	0	ID=BBa_K176006;Name=BBa_K176006;Parent=BBa_K176237
BBa_K176243	.	terminator	1452	1580	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K176006
BBa_K176243	.	terminator	1540	1580	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176243	.	stem_loop	1547	1566	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176243	.	polyA_site	1567	1580	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176243	.	stop_codon	1573	1573	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176243	.	engineered_region	1540	1580	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176243	.	terminator	1452	1531	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176243	.	stem_loop	1463	1506	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176243	.	engineered_region	1452	1531	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176243	.	engineered_region	741	1443	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K176006
BBa_K176243	.	ribosome_entry_site	741	752	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K176243	.	conserved_region	745	748	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176243	.	CDS	759	1418	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K176243	.	engineered_tag	1419	1443	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K176243	.	CDS	762	1378	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K176243	.	tag	1379	1412	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
>BBa_K176243
ggtaggtcacagggcaattttactagagtttacggctagctcagtcctaggtattatgctagctactagagaaagagga
gaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagt
cttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgata
actcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacagg
tgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaatta
agtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagcta
tatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcg
tattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatg
cctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactct
gatagtgctagtgtagatctctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaa
cagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagag
cagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcacc
atactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgcttt
actaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaa
ttagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttag
gttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccatt
attacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgc
ggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacact
gatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagtttatagctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgactataa
tgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaa
gcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatt
tatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtg
tttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaa
aaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagt
caaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatc
gtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaa
aaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatct
ctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagc
tcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatac
agaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtac
attgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccc
taaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcat
tcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacat
caggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcaga
aaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataat
tatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcat
gcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgc
gcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatac
tttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaat
atccctatcagtgatagaga