LOCUS BBa_K176246 4802 bp DNA linear 10-Dec-2025 DEFINITION High input score automatic selector (with reporter): PoPS->mRFP->K176229 ACCESSION BBa_K176246 VERSION BBa_K176246.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..693 /label=mrfp1 misc_feature 700..724 /label=Help:Barcodes CDS 19..699 /label=BBa_E1010 misc_feature 1..724 /label=BBa_I13502 misc_feature 737..740 /label=conserved RBS 733..744 /label=BBa_B0034 misc_feature 1330..1361 /label=LVA CDS 751..1329 /label=luxI misc_feature 751..1368 /label=BBa_C0061 misc_feature 1369..1393 /label=Help:Barcodes CDS 751..1368 /label=BBa_C0061 misc_feature 733..1393 /label=BBa_C0261 misc_feature 1..1393 /label=BBa_K176223 misc_feature 1406..1409 /label=conserved RBS 1402..1413 /label=BBa_B0034 CDS 1423..2039 /label=tetR misc_feature 2040..2073 /label=SsrA misc_feature 2080..2104 /label=Help:Barcodes CDS 1420..2079 /label=BBa_C0040 misc_feature 1402..2104 /label=BBa_S0101 stem_loop 2124..2167 /label=stem_loop misc_feature 2113..2192 /label=BBa_B0010 terminator 2113..2192 /label=BBa_B0010 stem_loop 2208..2227 /label=T7 TE misc_feature 2234..2234 /label=stop polyA_site 2228..2241 /label=polya misc_feature 2201..2241 /label=BBa_B0012 terminator 2201..2241 /label=BBa_B0012 terminator 2113..2241 /label=BBa_B0015 misc_feature 1402..2241 /label=BBa_K176006 promoter 2250..2255 /label=-35 promoter 2273..2278 /label=-10 misc_feature 2266..2266 /label=c in J23115 misc_feature 2270..2270 /label=t in J23115 misc_feature 2256..2261 /label=NheI misc_feature 2279..2284 /label=NheI misc_feature 2267..2272 /label=AvrII promoter 2250..2284 /label=BBa_K176008 misc_feature 2297..2300 /label=conserved RBS 2293..2304 /label=BBa_B0034 misc_feature 2890..2921 /label=LVA CDS 2311..2889 /label=luxI misc_feature 2311..2928 /label=BBa_C0061 misc_feature 2929..2953 /label=Help:Barcodes CDS 2311..2928 /label=BBa_C0061 misc_feature 2293..2953 /label=BBa_C0261 stem_loop 2973..3016 /label=stem_loop misc_feature 2962..3041 /label=BBa_B0010 terminator 2962..3041 /label=BBa_B0010 stem_loop 3057..3076 /label=T7 TE misc_feature 3083..3083 /label=stop polyA_site 3077..3090 /label=polya misc_feature 3050..3090 /label=BBa_B0012 terminator 3050..3090 /label=BBa_B0012 terminator 2962..3090 /label=BBa_B0015 misc_feature 2293..3090 /label=BBa_K082014 misc_feature 2250..3090 /label=BBa_K176022 promoter 3099..3133 /label=BBa_J23101 misc_feature 3146..3149 /label=conserved RBS 3142..3153 /label=BBa_B0034 misc_feature 3160..3161 /label=prefix misc_feature 3333..3333 /label=T misc_feature 3651..3651 /label=A CDS 3160..3909 /label=luxR misc_feature 3160..3915 /label=BBa_C0062 misc_feature 3916..3940 /label=Help:Barcodes CDS 3160..3915 /label=BBa_C0062 stem_loop 3960..4003 /label=stem_loop misc_feature 3949..4028 /label=BBa_B0010 terminator 3949..4028 /label=BBa_B0010 stem_loop 4044..4063 /label=T7 TE misc_feature 4070..4070 /label=stop polyA_site 4064..4077 /label=polya misc_feature 4037..4077 /label=BBa_B0012 terminator 4037..4077 /label=BBa_B0012 misc_feature 3142..4077 /label=BBa_I0462 misc_feature 3099..4077 /label=BBa_K176025 misc_feature 4086..4138 /label=R0062 promoter 4105..4110 /label=-35 promoter 4127..4132 /label=-10 misc_feature 4139..4157 /label=tetR misc_feature 4138..4138 /label=+1 misc_feature 4086..4105 /label=luxR/HSL promoter 4086..4157 /label=BBa_K176000 misc_feature 3099..4157 /label=BBa_K176028 misc_feature 2250..4157 /label=BBa_K176085 misc_feature 4172..4175 /label=conserved RBS 4166..4179 /label=BBa_B0031 misc_feature 4186..4350 /label=a part of lacZalpha (I732006) misc_feature 4186..4188 /label=Start misc_feature 4201..4201 /label=177bp and a in J32026 primer_bind 4206..4222 /label=M13 -20 primer primer_bind 4226..4249 /label=M13 -47 primer (K176059) primer_bind 4226..4242 /label=M13 -40 primer misc_feature 4350..4350 /label=g and 69bp in I732006 misc_feature 4357..4358 /label=at in K145151 misc_feature 4357..4662 /label=ccdB (K145151) misc_feature 4440..4440 /label=c in K145151 misc_feature 4660..4665 /label=Stop primer_bind 4373..4400 /label=VRccdB primer (K176058) CDS 4186..4659 /label=lacZalpha-ccdB CDS 4186..4665 /label=BBa_K176003 stem_loop 4685..4728 /label=stem_loop misc_feature 4674..4753 /label=BBa_B0010 terminator 4674..4753 /label=BBa_B0010 stem_loop 4769..4788 /label=T7 TE misc_feature 4795..4795 /label=stop polyA_site 4789..4802 /label=polya misc_feature 4762..4802 /label=BBa_B0012 terminator 4762..4802 /label=BBa_B0012 misc_feature 4166..4802 /label=BBa_K176063 misc_feature 2250..4802 /label=BBa_K176096 misc_feature 1402..4802 /label=BBa_K176226 ORIGIN 1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc 61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa 121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg 181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa 241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa 301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa 361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg 421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac 481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac 541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa 601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac 661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga 721 tcgctactag agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt 781 ttggcaattc catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag 841 caaagacttg agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac 901 tcaaatgcag aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt 961 ttattaccta caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa 1021 cagagtgctc ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat 1081 agctcaaaga taaataactc tgctagtgaa attacaatga aactatttga agctatatat 1141 aaacacgctg ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag 1201 cgatttttaa agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta 1261 ttaggtgata ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca 1321 gtcttaaatg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 1381 tagtgtagat ctctactaga gaaagaggag aaatactaga tgtccagatt agataaaagt 1441 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc 1501 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat 1561 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt 1621 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga 1681 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa 1741 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta 1801 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa 1861 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg 1921 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta 1981 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc 2041 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga 2101 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2161 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2221 gtgggccttt ctgcgtttat atactagagt ttatagctag ctcagtccta ggtacaatgc 2281 tagctactag agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt 2341 ttggcaattc catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag 2401 caaagacttg agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac 2461 tcaaatgcag aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt 2521 ttattaccta caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa 2581 cagagtgctc ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat 2641 agctcaaaga taaataactc tgctagtgaa attacaatga aactatttga agctatatat 2701 aaacacgctg ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag 2761 cgatttttaa agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta 2821 ttaggtgata ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca 2881 gtcttaaatg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 2941 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3001 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3061 caccttcggg tgggcctttc tgcgtttata tactagagtt tacagctagc tcagtcctag 3121 gtattatgct agctactaga gaaagaggag aaatactaga tgaaaaacat aaatgccgac 3181 gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga tattaatcaa 3241 tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc gatcatttat 3301 cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa aaaatggagg 3361 caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta ttctaactcc 3421 aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa aaaatctcca 3481 aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt ccctattcat 3541 acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga caactatata 3601 gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc tctagttgat 3661 aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac caaaagagaa 3721 aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc aaaaatatta 3781 ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa actcaataca 3841 acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga ttgcccatac 3901 tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa 3961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 4021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 4081 tagagacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaatatc 4141 cctatcagtg atagagatac tagagtcaca caggaaacct actagatgac catgattacg 4201 gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 4261 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 4321 gatcgccctt cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat 4381 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg 4441 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt 4501 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg 4561 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat 4621 gacatcaaaa acgccattaa cctgatgttc tggggaatat aataatacta gagccaggca 4681 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 4741 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 4801 ta //