##gff-version 3
##sequence-region BBa_K176246 1 4802
BBa_K176246	.	engineered_region	1402	4802	.	+	0	ID=BBa_K176226;Name=BBa_K176226
BBa_K176246	.	engineered_region	1402	2241	.	+	0	ID=BBa_K176006;Name=BBa_K176006;Parent=BBa_K176226
BBa_K176246	.	terminator	2113	2241	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K176006
BBa_K176246	.	terminator	2113	2192	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176246	.	stem_loop	2124	2167	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176246	.	engineered_region	2113	2192	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176246	.	terminator	2201	2241	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176246	.	stem_loop	2208	2227	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176246	.	engineered_region	2201	2241	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176246	.	stop_codon	2234	2234	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176246	.	polyA_site	2228	2241	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176246	.	engineered_region	1402	2104	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K176006
BBa_K176246	.	ribosome_entry_site	1402	1413	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K176246	.	conserved_region	1406	1409	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176246	.	CDS	1420	2079	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K176246	.	engineered_tag	2080	2104	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K176246	.	CDS	1423	2039	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K176246	.	tag	2040	2073	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K176246	.	engineered_region	2250	4802	.	+	0	ID=BBa_K176096;Name=BBa_K176096;Parent=BBa_K176226
BBa_K176246	.	engineered_region	2250	4157	.	+	0	ID=BBa_K176085;Name=BBa_K176085;Parent=BBa_K176096
BBa_K176246	.	engineered_region	2250	3090	.	+	0	ID=BBa_K176022;Name=BBa_K176022;Parent=BBa_K176085
BBa_K176246	.	promoter	2250	2284	.	+	0	ID=BBa_K176008;Name=BBa_K176008;Parent=BBa_K176022
BBa_K176246	.	sequence_alteration	2270	2270	.	+	0	ID=annotation2005562;Name=t in J23115;Parent=BBa_K176008
BBa_K176246	.	sequence_feature	2267	2272	.	+	0	ID=annotation2005565;Name=AvrII;Parent=BBa_K176008
BBa_K176246	.	promoter	2273	2278	.	+	0	ID=annotation2005560;Name=-10;Parent=BBa_K176008
BBa_K176246	.	sequence_feature	2279	2284	.	+	0	ID=annotation2005564;Name=NheI;Parent=BBa_K176008
BBa_K176246	.	sequence_feature	2256	2261	.	+	0	ID=annotation2005563;Name=NheI;Parent=BBa_K176008
BBa_K176246	.	sequence_alteration	2266	2266	.	+	0	ID=annotation2005561;Name=c in J23115;Parent=BBa_K176008
BBa_K176246	.	promoter	2250	2255	.	+	0	ID=annotation2005559;Name=-35;Parent=BBa_K176008
BBa_K176246	.	engineered_region	2293	3090	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K176022
BBa_K176246	.	terminator	2962	3090	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K176246	.	terminator	2962	3041	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176246	.	stem_loop	2973	3016	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176246	.	engineered_region	2962	3041	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176246	.	terminator	3050	3090	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176246	.	stem_loop	3057	3076	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176246	.	engineered_region	3050	3090	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176246	.	stop_codon	3083	3083	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176246	.	polyA_site	3077	3090	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176246	.	engineered_region	2293	2953	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K176246	.	CDS	2311	2928	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176246	.	engineered_tag	2929	2953	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176246	.	engineered_region	2311	2928	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176246	.	CDS	2311	2889	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176246	.	tag	2890	2921	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176246	.	ribosome_entry_site	2293	2304	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176246	.	conserved_region	2297	2300	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176246	.	engineered_region	3099	4157	.	+	0	ID=BBa_K176028;Name=BBa_K176028;Parent=BBa_K176085
BBa_K176246	.	engineered_region	3099	4077	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K176246	.	promoter	3099	3133	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176246	.	engineered_region	3142	4077	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176246	.	CDS	3160	3915	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176246	.	CDS	3160	3909	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176246	.	engineered_region	3160	3161	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176246	.	engineered_tag	3916	3940	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176246	.	point_mutation	3651	3651	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176246	.	engineered_region	3160	3915	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176246	.	point_mutation	3333	3333	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176246	.	ribosome_entry_site	3142	3153	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176246	.	conserved_region	3146	3149	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176246	.	terminator	4037	4077	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176246	.	stem_loop	4044	4063	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176246	.	engineered_region	4037	4077	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176246	.	stop_codon	4070	4070	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176246	.	polyA_site	4064	4077	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176246	.	terminator	3949	4028	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176246	.	stem_loop	3960	4003	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176246	.	engineered_region	3949	4028	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176246	.	promoter	4086	4157	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K176246	.	start_codon	4138	4138	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176246	.	non_covalent_binding_site	4086	4105	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176246	.	promoter	4127	4132	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176246	.	promoter	4105	4110	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176246	.	non_covalent_binding_site	4139	4157	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176246	.	engineered_region	4086	4138	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176246	.	engineered_region	4166	4802	.	+	0	ID=BBa_K176063;Name=BBa_K176063;Parent=BBa_K176096
BBa_K176246	.	CDS	4186	4665	.	+	0	ID=BBa_K176003;Name=BBa_K176003;Parent=BBa_K176063
BBa_K176246	.	point_mutation	4350	4350	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176246	.	primer_binding_site	4226	4249	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176246	.	start_codon	4186	4188	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K176246	.	CDS	4186	4659	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176246	.	engineered_region	4357	4662	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176246	.	primer_binding_site	4206	4222	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K176246	.	primer_binding_site	4226	4242	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176246	.	stop_codon	4660	4665	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176246	.	point_mutation	4440	4440	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176246	.	sequence_alteration	4357	4358	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176246	.	engineered_region	4186	4350	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176246	.	sequence_alteration	4201	4201	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176246	.	primer_binding_site	4373	4400	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176246	.	ribosome_entry_site	4166	4179	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K176063
BBa_K176246	.	conserved_region	4172	4175	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176246	.	terminator	4674	4753	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176063
BBa_K176246	.	stem_loop	4685	4728	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176246	.	engineered_region	4674	4753	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176246	.	terminator	4762	4802	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176063
BBa_K176246	.	stem_loop	4769	4788	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176246	.	engineered_region	4762	4802	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176246	.	stop_codon	4795	4795	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176246	.	polyA_site	4789	4802	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176246	.	engineered_region	1	1393	.	+	0	ID=BBa_K176223;Name=BBa_K176223
BBa_K176246	.	engineered_region	1	724	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_K176223
BBa_K176246	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_K176246	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176246	.	CDS	19	699	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_K176246	.	CDS	19	693	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K176246	.	engineered_tag	700	724	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K176246	.	engineered_region	733	1393	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K176223
BBa_K176246	.	CDS	751	1368	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K176246	.	engineered_tag	1369	1393	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K176246	.	engineered_region	751	1368	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K176246	.	CDS	751	1329	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K176246	.	tag	1330	1361	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K176246	.	ribosome_entry_site	733	744	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K176246	.	conserved_region	737	740	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K176246
aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggtt
ccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaa
agttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtt
aaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcg
aagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg
taccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccg
gaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaa
ccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaa
cgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgc
tagtgtagatcgctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattc
catcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtaga
aaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagt
ggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtg
ctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctag
tgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaaca
tcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattag
gtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacga
aaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatg
tccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaaccc
gtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgc
cttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgt
aataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacag
aaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgc
actcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaa
acacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcct
tcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacga
aaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagtttatagctagctcagtcctaggtacaatgctagctactaga
gaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggt
attctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatg
agtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacc
tacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatata
gtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactat
ttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatt
tttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgta
ttgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctt
aataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactaga
tgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatca
atgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaa
tctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatc
ctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatc
tccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggc
ttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatac
cattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaa
aagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgag
cgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaa
ttttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggttta
cgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagtcacacaggaaacctactagat
gaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
cttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgca
gcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtga
tattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaa
ctttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgtta
tcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatata
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata