LOCUS BBa_K176248 5797 bp DNA linear 22-Nov-2024 DEFINITION Low input score automatic selector (with reporter): PoPS->mRFP->CI Inverter (cI-LVA+pCI)->K176229 ACCESSION BBa_K176248 VERSION BBa_K176248.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..693 /label=mrfp1 misc_feature 700..724 /label=Help:Barcodes CDS 19..699 /label=BBa_E1010 misc_feature 1..724 /label=BBa_I13502 misc_feature 737..740 /label=conserved RBS 733..744 /label=BBa_B0034 CDS 754..1461 /label=cI lambda misc_feature 1462..1494 /label=LVA misc_feature 1501..1525 /label=Help:Barcodes CDS 751..1500 /label=BBa_C0051 stem_loop 1545..1588 /label=stem_loop misc_feature 1534..1613 /label=BBa_B0010 terminator 1534..1613 /label=BBa_B0010 stem_loop 1629..1648 /label=T7 TE misc_feature 1655..1655 /label=stop polyA_site 1649..1662 /label=polya misc_feature 1622..1662 /label=BBa_B0012 terminator 1622..1662 /label=BBa_B0012 promoter 1708..1713 /label=-10 promoter 1685..1690 /label=-35 misc_feature 1695..1711 /label=OR1 misc_feature 1671..1687 /label=OR2 misc_feature 1671..1719 /label=BBa_R0051 promoter 1671..1719 /label=BBa_R0051 misc_feature 733..1719 /label=BBa_Q04510 misc_feature 1732..1735 /label=conserved RBS 1728..1739 /label=BBa_B0034 misc_feature 2325..2356 /label=LVA CDS 1746..2324 /label=luxI misc_feature 1746..2363 /label=BBa_C0061 misc_feature 2364..2388 /label=Help:Barcodes CDS 1746..2363 /label=BBa_C0061 misc_feature 1728..2388 /label=BBa_C0261 misc_feature 733..2388 /label=BBa_K176189 misc_feature 1..2388 /label=BBa_K176224 misc_feature 2401..2404 /label=conserved RBS 2397..2408 /label=BBa_B0034 CDS 2418..3034 /label=tetR misc_feature 3035..3068 /label=SsrA misc_feature 3075..3099 /label=Help:Barcodes CDS 2415..3074 /label=BBa_C0040 misc_feature 2397..3099 /label=BBa_S0101 stem_loop 3119..3162 /label=stem_loop misc_feature 3108..3187 /label=BBa_B0010 terminator 3108..3187 /label=BBa_B0010 stem_loop 3203..3222 /label=T7 TE misc_feature 3229..3229 /label=stop polyA_site 3223..3236 /label=polya misc_feature 3196..3236 /label=BBa_B0012 terminator 3196..3236 /label=BBa_B0012 terminator 3108..3236 /label=BBa_B0015 misc_feature 2397..3236 /label=BBa_K176006 promoter 3245..3250 /label=-35 promoter 3268..3273 /label=-10 misc_feature 3261..3261 /label=c in J23115 misc_feature 3265..3265 /label=t in J23115 misc_feature 3251..3256 /label=NheI misc_feature 3274..3279 /label=NheI misc_feature 3262..3267 /label=AvrII promoter 3245..3279 /label=BBa_K176008 misc_feature 3292..3295 /label=conserved RBS 3288..3299 /label=BBa_B0034 misc_feature 3885..3916 /label=LVA CDS 3306..3884 /label=luxI misc_feature 3306..3923 /label=BBa_C0061 misc_feature 3924..3948 /label=Help:Barcodes CDS 3306..3923 /label=BBa_C0061 misc_feature 3288..3948 /label=BBa_C0261 stem_loop 3968..4011 /label=stem_loop misc_feature 3957..4036 /label=BBa_B0010 terminator 3957..4036 /label=BBa_B0010 stem_loop 4052..4071 /label=T7 TE misc_feature 4078..4078 /label=stop polyA_site 4072..4085 /label=polya misc_feature 4045..4085 /label=BBa_B0012 terminator 4045..4085 /label=BBa_B0012 terminator 3957..4085 /label=BBa_B0015 misc_feature 3288..4085 /label=BBa_K082014 misc_feature 3245..4085 /label=BBa_K176022 promoter 4094..4128 /label=BBa_J23101 misc_feature 4141..4144 /label=conserved RBS 4137..4148 /label=BBa_B0034 misc_feature 4155..4156 /label=prefix misc_feature 4328..4328 /label=T misc_feature 4646..4646 /label=A CDS 4155..4904 /label=luxR misc_feature 4155..4910 /label=BBa_C0062 misc_feature 4911..4935 /label=Help:Barcodes CDS 4155..4910 /label=BBa_C0062 stem_loop 4955..4998 /label=stem_loop misc_feature 4944..5023 /label=BBa_B0010 terminator 4944..5023 /label=BBa_B0010 stem_loop 5039..5058 /label=T7 TE misc_feature 5065..5065 /label=stop polyA_site 5059..5072 /label=polya misc_feature 5032..5072 /label=BBa_B0012 terminator 5032..5072 /label=BBa_B0012 misc_feature 4137..5072 /label=BBa_I0462 misc_feature 4094..5072 /label=BBa_K176025 misc_feature 5081..5133 /label=R0062 promoter 5100..5105 /label=-35 promoter 5122..5127 /label=-10 misc_feature 5134..5152 /label=tetR misc_feature 5133..5133 /label=+1 misc_feature 5081..5100 /label=luxR/HSL promoter 5081..5152 /label=BBa_K176000 misc_feature 4094..5152 /label=BBa_K176028 misc_feature 3245..5152 /label=BBa_K176085 misc_feature 5167..5170 /label=conserved RBS 5161..5174 /label=BBa_B0031 misc_feature 5181..5345 /label=a part of lacZalpha (I732006) misc_feature 5181..5183 /label=Start misc_feature 5196..5196 /label=177bp and a in J32026 primer_bind 5201..5217 /label=M13 -20 primer primer_bind 5221..5244 /label=M13 -47 primer (K176059) primer_bind 5221..5237 /label=M13 -40 primer misc_feature 5345..5345 /label=g and 69bp in I732006 misc_feature 5352..5353 /label=at in K145151 misc_feature 5352..5657 /label=ccdB (K145151) misc_feature 5435..5435 /label=c in K145151 misc_feature 5655..5660 /label=Stop primer_bind 5368..5395 /label=VRccdB primer (K176058) CDS 5181..5654 /label=lacZalpha-ccdB CDS 5181..5660 /label=BBa_K176003 stem_loop 5680..5723 /label=stem_loop misc_feature 5669..5748 /label=BBa_B0010 terminator 5669..5748 /label=BBa_B0010 stem_loop 5764..5783 /label=T7 TE misc_feature 5790..5790 /label=stop polyA_site 5784..5797 /label=polya misc_feature 5757..5797 /label=BBa_B0012 terminator 5757..5797 /label=BBa_B0012 misc_feature 5161..5797 /label=BBa_K176063 misc_feature 3245..5797 /label=BBa_K176096 misc_feature 2397..5797 /label=BBa_K176226 ORIGIN 1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc 61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa 121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg 181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa 241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa 301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa 361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg 421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac 481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac 541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa 601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac 661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga 721 tcgctactag agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa 781 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt 841 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct 901 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc 961 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa 1021 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt 1081 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg 1141 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc 1201 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac 1261 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt 1321 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag 1381 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag 1441 tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa 1501 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc 1561 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 1621 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg 1681 cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat 1741 actagatgac tataatgata aaaaaatcgg attttttggc aattccatcg gaggagtata 1801 aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg gacttagttg 1861 tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat atttatgctt 1921 gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca ggtgattata 1981 tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa gatcctaata 2041 tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc aaagataaat aactctgcta 2101 gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt caaggtatta 2161 cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt attaaagttc 2221 cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa tcggttgtat 2281 tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaatgctgca aacgacgaaa 2341 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta ctagagaaag 2401 aggagaaata ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc 2461 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg 2521 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag 2581 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc 2641 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg 2701 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc 2761 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg 2821 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag 2881 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat 2941 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg 3001 gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt 3061 tagtagctta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat 3121 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 3181 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 3241 agagtttata gctagctcag tcctaggtac aatgctagct actagagaaa gaggagaaat 3301 actagatgac tataatgata aaaaaatcgg attttttggc aattccatcg gaggagtata 3361 aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg gacttagttg 3421 tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat atttatgctt 3481 gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca ggtgattata 3541 tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa gatcctaata 3601 tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc aaagataaat aactctgcta 3661 gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt caaggtatta 3721 cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt attaaagttc 3781 cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa tcggttgtat 3841 tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaatgctgca aacgacgaaa 3901 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag 3961 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 4021 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 4081 ttatatacta gagtttacag ctagctcagt cctaggtatt atgctagcta ctagagaaag 4141 aggagaaata ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa 4201 ttaaagcttg tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg 4261 tacattgtga atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata 4321 tttcaatcct agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa 4381 taaaatatga tcctatagta gattattcta actccaatca ttcaccaatt aattggaata 4441 tatttgaaaa caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat 4501 caggtcttat cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc 4561 ttagttttgc acattcagaa aaagacaact atatagatag tttattttta catgcgtgta 4621 tgaacatacc attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa 4681 ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg 4741 aaggaaaaag ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt 4801 tccatttaac caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta 4861 aagcaatttt aacaggagca attgattgcc catactttaa aaattaataa cactgatagt 4921 gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 4981 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 5041 ctcaccttcg ggtgggcctt tctgcgttta tatactagag acctgtagga tcgtacaggt 5101 ttacgcaaga aaatggtttg ttatagtcga atatccctat cagtgataga gatactagag 5161 tcacacagga aacctactag atgaccatga ttacggattc actggccgtc gttttacaac 5221 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 5281 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 5341 gcctatacgt acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt 5401 ttgtggatgt acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg 5461 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg 5521 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg 5581 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga 5641 tgttctgggg aatataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 5701 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 5761 actggctcac cttcgggtgg gcctttctgc gtttata //