LOCUS       BBa_K1763006            1524 bp    DNA     linear       30-Dec-2025
DEFINITION  Honeybee silk SpyCatcher fusion
ACCESSION   BBa_K1763006
VERSION     BBa_K1763006.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start codon
     CDS             227..1177
                     /label=Honey bee silk coding region
     CDS             227..1177
                     /label=BBa_K1763000
     CDS             1186..1524
                     /label=N-terminal FbaB (modified SpyCatcher)
     misc_feature    1285..1287
                     /label=Iso --> Glu
     misc_feature    1390..1392
                     /label=Met --> Tyr
     misc_feature    1276..1278
                     /label=Reactive Lys for isopeptide forming reaction
     CDS             1186..1524
                     /label=BBa_K1159200
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg gcgtcgagga
      241 gttcaagtcc tcggcaaccg aggaggtgat cagcaaaaac ttagaagtcg acctgttgaa
      301 aaatgtggac actagcgcga aacgaagaga gaacggcgcc ccggtgctcg gcaagaacac
      361 acttcaatcc ctggagaaga tcaagacgtc ggcgagcgtg aatgccaaag cagcagccgt
      421 ggtgaaagcg tccgctctgg ctcttgcaga ggcctatttg cgagcgtccg cattgtcagc
      481 cgccgcttca gccaaggcag ccgccgccct gaaaaatgct caacaagcgc aattaaacgc
      541 ccaggaaaag tctttggccg cgttgaaagc tcagtccgag gaagaggcag cttctgctcg
      601 tgcaaacgca gcaaccgccg cgacacagtc ggcactggaa cgcgctcaag cctcctccag
      661 gttagcaacg gtcgcccaaa acgtagccag cgacttgcag aaacggacca gcaccaaggc
      721 cgcggctgaa gccgctgcca ccctcagaca attacaggac gcggaacgaa cgaaatggag
      781 tgccaacgct gccttagaag tctccgccgc tgcggctgcc gcagaaacca agaccactgc
      841 ctcctcggag gccgccaacg ccgccgccaa aaaggcggca gcaatagctt ctgacgcgga
      901 cggcgcggaa aggtcggcat ctaccgaggc acaatcagct gcgaagatcg agagtgtggc
      961 agccgccgag ggaagcgcca actcggcctc tgaggattcc cgggccgctc aattggaagc
     1021 ctccaccgcg gcgagagcca acgtggccgc agctgtcggg gatggagcga ttataggact
     1081 tggagaggaa gcgggtgccg cggctcagtt gcttgcacag gcgaaggcat tggccgaagt
     1141 tagctcgaaa tccgagaaca tcgaggacaa gaagttctac tagaggttga taccttatca
     1201 ggtttatcaa gtgagcaagg tcagtccggt gatatgacaa ttgaagaaga tagtgctacc
     1261 catattaaat tctcaaaacg tgatgaggac ggcaaagagt tagctggtgc aactatggag
     1321 ttgcgtgatt catctggtaa aactattagt acatggattt cagatggaca agtgaaagat
     1381 ttctacctgt atccaggaaa atatacattt gtcgaaaccg cagcaccaga cggttatgag
     1441 gtagcaactg ctattacctt tacagttaat gagcaaggtc aggttactgt aaatggcaaa
     1501 gcaactaaag gtgacgctca tatt
//