LOCUS       BBa_K1763444            1639 bp    DNA     linear       26-Dec-2024
DEFINITION  Bombyx mori silk co-spinning module
ACCESSION   BBa_K1763444
VERSION     BBa_K1763444.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=LacI (BBa_R0010)
     misc_feature    201..208
                     /label=Bba prefix/suffix assembly scar site
     RBS             209..220
                     /label=Elowitz RBS (BBa_B0034)
     misc_feature    230..247
                     /label=6x N-terminal polyhistidine tag
     CDS             248..712
                     /label=B. mori FibNT
     misc_feature    719..724
                     /label=BsaI Restriction Site (EcoRI Compatible)
     CDS             725..1438
                     /label=superfolder GFP (BBa_I746916)
     CDS             1451..1627
                     /label=B. mori FibCT
     misc_feature    1628..1633
                     /label=Ribosome Double-Stop Site
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa ggatccatgc atcatcacca
      241 ccatcatatg cgtgtgaaaa cgtttgtgat tttatgctgt gcccttcaat acgtagccta
      301 tactaacgcg aacatcaatg atttcgacga ggactatttc gggagtgatg ttactgttca
      361 gtcgagcaac accacggatg aaatcatccg tgacgcatca ggcgctgtca ttgaggagca
      421 gatcactacg aagaaaatgc aacgcaaaaa caaaaatcat ggtattcttg gcaaaaacga
      481 aaagatgatc aaaacctttg ttattacgac tgattctgat ggtaatgagt cgattgtcga
      541 agaagatgtg ctgatgaaga ccctgtcaga tggtacggtc gcacagtctt acgtcgcagc
      601 ggatgcgggc gcatactcac aaagcggacc ttatgtcagc aactgcggat actccaccca
      661 ccaaggttac acctctgact tcagcacaag tgccgcggtg ggtgcgggct cgcaattgga
      721 gaccatgcgt aaaggtgagg agctgtttac gggcgtggtc ccgattctgg ttgagctcga
      781 tggtgatgta aacggtcaca agttctcggt acgcggtgaa ggcgaaggag acgccaccaa
      841 tggcaaatta accctcaaat tcatttgcac caccggcaag ctgccggtgc cgtggccgac
      901 cttggtaacc accctgactt acggtgttca gtgtttcgct cgttatccag atcacatgaa
      961 acagcatgat ttttttaaat cagcgatgcc ggaaggctac gtacaggaac gtaccatcag
     1021 cttcaaagat gatggtacct ataaaactcg tgcggaagtc aaattcgagg gcgataccct
     1081 ggtaaaccgt attgaactca agggaatcga ctttaaggaa gatggtaaca ttttgggcca
     1141 caaactcgaa tacaatttta actcccataa cgtttacatc accgcggaca aacagaaaaa
     1201 tggtatcaag gcgaatttca agatccgcca taatgtggag gatggtagtg tgcaattagc
     1261 ggaccactat cagcaaaaca ccccgattgg tgatgggcct gtgcttcttc ctgataatca
     1321 ttacctgtca acgcaaagcg ttttatcgaa agatccgaac gaaaaacgtg accacatggt
     1381 tctgttggaa tttgttacag ccgccggaat cacgcatggt atggacgagc tgtacaaagg
     1441 tctcgctaga tctgttagct atggcgcggg acgcggttac ggccaagggg caggctcggc
     1501 ggcctcgtcg gtgtccagcg cgtcgtcccg ctcgtacgac tattcccgcc gcaatgtgcg
     1561 caaaaactgt ggaatcccgc gccgccaact ggtggtgaaa tttcgtgcac tgccttgcgt
     1621 caactgttaa taaaagctt
//