LOCUS BBa_K1765000 5360 bp DNA linear 23-Dec-2024 DEFINITION Pconst(low)-G3DH-T0 terminator-Pconst(low)-OtsB-OtsA-T0 terminator ACCESSION BBa_K1765000 VERSION BBa_K1765000.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23109 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..2772 /label=BBa_K1339005 terminator 2781..2884 /label=BBa_K1339025 misc_feature 44..2884 /label=BBa_K1339020 misc_feature 1..2884 /label=BBa_K1339024 misc_feature 2893..5360 /label=BBa_K1339018 ORIGIN 1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaaagagg agaaatacta 61 gatggcagac aatcattatg atgcgattgt tgtcggctca ggcatcagtg gaggctgggc 121 cgcgaaggaa ctcacgcaaa agggcctgaa agtcctgatg ctggagcgtg gccgcaatat 181 cgagcacgtc accgactacc agaatgccga caaggaagcc tgggattatc cccatcgcaa 241 ccgcgccacg caggagatga aggcgaaata tcccgtcctc agccgcgatt acctgctgga 301 agaagcaaca ctcggcatgt gggccgatga gcaggaaacg ccctatgtcg aggaaaagcg 361 cttcgactgg ttccgcggtt atcatgtcgg cggccgctca ttgctctggg ggcgccagac 421 ctatcgctgg tcgcagaccg attttgaggc caatgcgaaa gacggcatcg ccgtcgactg 481 gccgatccgt tatgaagacg tctccccctg gtatgactat gtcgagcgtt ttgccggcat 541 ctccggcagc cgcgaggggt tggatattct tcccgatggc gagtttctgc cgcccattcc 601 gctcaatttc gtcgagcagg atgtcgccag ccggctgaag aaggctttca agggtacgcg 661 ccacctcatc aattcgcgct gcgccaacat tacccaggag cttcccgatc aggagcgcac 721 cccctgtcag ttccgcaaca agtgtcggct gggctgtccc ttcggcggct atttcagcac 781 gcaggcctcg accctgcctg cggccgtcgc caccggtaat ctgacgctgc ggcccttctc 841 catcgtcaag gaaatcctct acgacaagga taagaagaag gcgcgcggtg tcgagatcat 901 cgatgccgag acgaacctga cctatgaata taccgccgac atcatcttcc tgaacgcctc 961 gacgctgaac tcgacatggg tgctgatgaa ctcggcgacc gatgtctggg aaggcgggct 1021 tggcagcagc tccggtgaac tcggccataa tgtgatggac caccatttcc gcatgggcgc 1081 gaccggccag gtggacggtt tcgaggactt ctatttcaag ggccgccgtc cggcgggctt 1141 ctacattccg cgtttccgca ataccggcga tgacaagcgc aaatatctgc gcggtttcgg 1201 ttatcaggga tcggccagcc gttcacgctg ggagcgggaa atcgccgagc taaacatcgg 1261 tgccgactat aaggataccc tgaccgagcc gggcggctgg accattggca tgaccgcctt 1321 cggcgaaatg ctgccctatc acgataaccg cgtgaagctc gatcatgaca agaaggacaa 1381 atggggcctg ccggtccttt ccatgaatgt cgagatgaag cagaacgaac tcgacatgcg 1441 tgaagatatg gtcaatgacg ccgtcgagat gttcgaggcg gtcggcatca agaacgtcaa 1501 gccctcgcgg ggtacctacg cccccggcat gggcattcac gaaatgggaa cggcccgcat 1561 gggacgcgac ccgaaaacct ccgtcctcaa cggcaacaac caggtctggg atgcacccaa 1621 tgtcttcgtc accgacggtg cctgcatgac ctctgcctcc tgcgttaacc cgtccttgac 1681 ctacatggcg ctgacggcgc gtgctgccga gtttgccgtt tccgaacgta agaaggggaa 1741 cctgtgatga acagacgcga actgttgaaa ctgatagcca ttgccacggg ccttccgctg 1801 atcggcgcgg atgtcctcac ggcggcggaa aatgccgcca aaccagccgg cggcggccat 1861 gtgttcagcc cggaggaaat ccggtttctg gacgaggtgg cggagaccat cattccccgc 1921 acctccacgc cgggtgcgaa ggatgctgaa gtgggtgcat tcatggccgt ctatgccgcc 1981 gattgttata ccgacgagca gcgggcgctc ttcctttcgg caatccccga aatagagaaa 2041 cgtagtcagg cagaccataa aaagggcttt ctggatctga cggcagaaca gaagcaggcg 2101 ttgctttctg cgctggacaa gcaggccagg gcggaacaga cgccggcaaa gccgcatgcc 2161 tttacgctgg tcaagcagtt gacgctgctc ggtttcttca cctcgaagat cggcgctacg 2221 gaagttctcg tctatgacga gattcccggt ggtttcgaag accgcgttcc ctacaagaag 2281 ggcacgcctg cctggggcac gacctgaacc gagaaccatc ggcccgtccg cctttgcggg 2341 cgggccgcat tcatgcggca agacaaggat gacacgatgc gcaatatatc aatcatcgcg 2401 gcagcgatgc tagcggccag caccgttccc gctctttcgg agggcgatgt tgccaagggg 2461 gaggcggtct tcaagcgctg ttccgcctgt cacgccatcg gcgagggcgc caaaaaccgg 2521 gtcggcccgc agcttaacgg catcatcggc cgcgcagcgg gcggcgtgcc tgattatact 2581 tattcgagcg cgatgaaaaa agcgggtgag gacgggctcg tctggacgcc ggaagaattg 2641 cgggatttcc tgagcgcgcc aaagaagaaa atacccggaa acaagatggc gctcgccggc 2701 atcagtaaac cggaagatct ggacaatctc atcgcctata tcgagagcac ggcttccaag 2761 ccggctgagt gatactagag gcttggactc ctgttgatag atccagtaat gacctcagaa 2821 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat 2881 ccaatactag agtttacagc tagctcagtc ctagggactg tgctagctac tagagtagtg 2941 gaggttacta gagaaagagg agaaatacta gatgacagaa ccgttaaccg aaacccctga 3001 actatccgcg aaatatgcct ggttttttga tcttgatgga acgctggcgg aaatcaaacc 3061 gcatcccgat caggtcgtcg tgcctgacaa tattctgcaa ggactacagc tactggcaac 3121 cgcaagtgat ggtgcattgg cattgatatc agggcgctca atggtggagc ttgacgcact 3181 ggcaaaacct tatcgcttcc cgttagcggg cgtgcatggg gcggagcgcc gtgacatcaa 3241 tggtaaaaca catatcgttc atctgccgga tgcgattgcg cgtgatatta gcgtgcaact 3301 gcatacagtc atcgctcagt atcccggcgc ggagctggag gcgaaaggga tggcttttgc 3361 gctgcattat cgtcaggctc cgcagcatga agacgcatta atgacattag cgcaacgtat 3421 tactcagatc tggccacaaa tggcgttaca gcagggaaag tgtgttgtcg agatcaaacc 3481 gagaggtacc agtaaaggtg aggcaattgc agcttttatg caggaagctc cctttatcgg 3541 gcgaacgccc gtatttctgg gcgatgattt aaccgatgaa tctggcttcg cagtcgttaa 3601 ccgactgggc ggaatgtcag taaaaattgg cacaggtgca actcaggcat catggcgact 3661 ggcgggtgtg ccggatgtct ggagctggct tgaaatgata accaccgcat tacaacaaaa 3721 aagagaaaat aacaggagtg atgactatga gtcgtttagt cgtagtatct aatactagag 3781 aaagaggaga aatactagat gagtcgttta gtcgtagtat ctaaccggat tgcaccacca 3841 gacgagcacg ccgccagtgc cggtggcctt gccgttggca tactgggggc actgaaagcc 3901 gcaggcggac tgtggtttgg ctggagtggt gaaacaggga atgaggatca gccgctaaaa 3961 aaggtgaaaa aaggtaacat tacgtgggcc tcttttaacc tcagcgaaca ggaccttgac 4021 gaatactaca accaattctc caatgccgtt ctctggcccg cttttcatta tcggctcgat 4081 ctggtgcaat ttcagcgtcc tgcctgggac ggctatctac gcgtaaatgc gttgctggca 4141 gataaattac tgccgctgtt gcaagacgat gacattatct ggatccacga ttatcacctg 4201 ttgccatttg cgcatgaatt acgcaaacgg ggagtgaata atcgcattgg tttctttctg 4261 catattcctt tcccgacacc ggaaatcttc aacgcgctgc cgacatatga caccttgctt 4321 gaacagcttt gtgattatga tttgctgggt ttccagacag aaaacgatcg tctggcgttc 4381 ctggattgtc tttctaacct gacccgcgtc acgacacgta gcgcaaaaag ccatacagcc 4441 tggggcaaag catttcgaac agaagtctac ccgatcggca ttgaaccgaa agaaatagcc 4501 aaacaggctg ccgggccact gccgccaaaa ctggcgcaac ttaaagcgga actgaaaaac 4561 gtacaaaata tcttttctgt cgaacggctg gattattcca aaggtttgcc agagcgtttt 4621 ctcgcctatg aagcgttgct ggaaaaatat ccgcagcatc atggtaaaat tcgttatacc 4681 cagattgcac caacgtcgcg tggtgatgtg caagcctatc aggatattcg tcatcagctc 4741 gaaaatgaag ctggacgaat taatggtaaa tacgggcaat taggctggac gccgctttat 4801 tatttgaatc agcattttga ccgtaaatta ctgatgaaaa tattccgcta ctctgacgtg 4861 ggcttagtga cgccactgcg tgacgggatg aacctggtag caaaagagta tgttgctgct 4921 caggacccag ccaatccggg cgttcttgtt ctttcgcaat ttgcgggagc ggcaaacgag 4981 ttaacgtcgg cgttaattgt taacccctac gatcgtgacg aagttgcagc tgcgctggat 5041 cgtgcattga ctatgtcgct ggcggaacgt atttcccgtc atgcagaaat gctggacgtt 5101 atcgtgaaaa acgatattaa ccactggcag gagtgcttca ttagcgacct aaagcagata 5161 gttccgcgaa gcgcggaaag ccagcagcgc gataaagttg ctacctttcc aaagcttgcg 5221 tagtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 5281 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 5341 tgggcctttc tgcgtttata //