LOCUS       BBa_K1766004            2422 bp    DNA     linear       23-May-2024
DEFINITION  OmpR controlled regulatory circuit producing RFP and GFP
ACCESSION   BBa_K1766004
VERSION     BBa_K1766004.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     CDS             135..809
                     /label=mrfp1
     misc_feature    816..840
                     /label=Help:Barcodes
     CDS             135..815
                     /label=BBa_E1010
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
     misc_feature    1..977
                     /label=BBa_M30011
     stem_loop       1950..1993
                     /label=stem_loop
     misc_feature    1939..2018
                     /label=BBa_B0010
     terminator      1939..2010
                     /label=BBa_B0010
     stem_loop       2026..2045
                     /label=T7 TE
     misc_feature    2052..2052
                     /label=stop
     polyA_site      2046..2059
                     /label=polya
     misc_feature    2019..2059
                     /label=BBa_B0012
     terminator      2019..2059
                     /label=BBa_B0012
     misc_feature    986..1169
                     /label=BBa_K1766001
     promoter        986..1020
                     /label=BBa_J23101
     misc_feature    1021..1169
                     /label=MicF target
     CDS             1131..1919
                     /label=OmpF-GFP fusion
     misc_feature    1203..1919
                     /label=BBa_E0040
     misc_feature    1939..2010
                     /label=BBa_B0010
     misc_feature    2019..2059
                     /label=BBa_B0012
     stem_loop       2026..2053
                     /label=T7 TE
     misc_feature    986..2059
                     /label=BBa_K1766002
     misc_feature    2080..2097
                     /label=C1 OmpR
     misc_feature    2101..2118
                     /label=C2 OmpR
     misc_feature    2121..2138
                     /label=C3 OmpR
     promoter        2142..2147
                     /label=-35
     promoter        2165..2170
                     /label=-10
     promoter        2068..2175
                     /label=BBa_R0082
     misc_feature    2330..2422
                     /label=MicF 
     misc_feature    2184..2422
                     /label=BBa_K1766000
     misc_feature    2068..2422
                     /label=BBa_K1766003
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag
      181 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc
      241 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt
      301 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc
      361 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg
      421 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg
      481 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta
      541 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg
      601 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg
      661 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg
      721 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac
      781 gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttatatac tagagtttac agctagctca gtcctaggta ttatgctagc
     1021 agacacataa agacaccaaa ctctcatcaa tagttccgta aatttttatt gacagaactt
     1081 attgacggca gtggcaggtg tcataaaaaa aaccatgagg gtaataaata atgatgaagc
     1141 gcaatattct ggcagtgatc gtccctgctt acttgagtca cacagaatgc ggaaagtact
     1201 tgatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     1261 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     1321 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     1381 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     1441 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     1501 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     1561 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     1621 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     1681 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1741 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1801 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1861 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1921 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1981 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2041 gggcctttct gcgtttatat actagagtcc cttgcattta cattttgaaa catctatagc
     2101 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
     2161 tggggagaat ggacttacta gagacttttt aagattattg cggaatggcg aaataagcac
     2221 ctaacatcaa gcaataataa ttcaaggtta aaatcaataa cttattctta agtatttgac
     2281 agcactgaat gtcaaaacaa aaccttcact cgcaactaga ataactcccg ctatcatcat
     2341 taactttatt tattaccgtc attcatttct gaatgtctgt ttacccctat ttcaaccgga
     2401 tgcctcgcat tcggtttttt tt
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