##gff-version 3
##sequence-region BBa_K1766004 1 2422
BBa_K1766004	.	engineered_region	1	977	.	+	0	ID=BBa_M30011;Name=BBa_M30011
BBa_K1766004	.	terminator	937	977	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_M30011
BBa_K1766004	.	polyA_site	964	977	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1766004	.	stem_loop	944	963	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1766004	.	engineered_region	937	977	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1766004	.	stop_codon	970	970	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1766004	.	terminator	849	928	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M30011
BBa_K1766004	.	stem_loop	860	903	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1766004	.	engineered_region	849	928	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1766004	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30011
BBa_K1766004	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1766004	.	CDS	135	815	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_M30011
BBa_K1766004	.	engineered_tag	816	840	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1766004	.	CDS	135	809	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1766004	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_M30011
BBa_K1766004	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K1766004	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K1766004	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K1766004	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K1766004	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K1766004	.	engineered_region	986	2059	.	+	0	ID=BBa_K1766002;Name=BBa_K1766002
BBa_K1766004	.	CDS	1131	1919	.	+	0	ID=annotation2445270;Name=OmpF-GFP fusion;Parent=BBa_K1766002
BBa_K1766004	.	engineered_region	1203	1919	.	+	0	ID=annotation2445271;Name=BBa_E0040;Parent=BBa_K1766002
BBa_K1766004	.	non_covalent_binding_site	1021	1169	.	+	0	ID=annotation2445269;Name=MicF target;Parent=BBa_K1766002
BBa_K1766004	.	engineered_region	986	1169	.	+	0	ID=annotation2445267;Name=BBa_K1766001;Parent=BBa_K1766002
BBa_K1766004	.	stem_loop	2026	2053	.	+	0	ID=annotation2445274;Name=T7 TE;Parent=BBa_K1766002
BBa_K1766004	.	engineered_region	1939	2010	.	+	0	ID=annotation2445272;Name=BBa_B0010;Parent=BBa_K1766002
BBa_K1766004	.	engineered_region	2019	2059	.	+	0	ID=annotation2445273;Name=BBa_B0012;Parent=BBa_K1766002
BBa_K1766004	.	promoter	986	1020	.	+	0	ID=annotation2445268;Name=BBa_J23101;Parent=BBa_K1766002
BBa_K1766004	.	terminator	1939	2010	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1766004	.	stem_loop	1950	1993	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1766004	.	engineered_region	1939	2018	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1766004	.	engineered_region	2068	2422	.	+	0	ID=BBa_K1766003;Name=BBa_K1766003
BBa_K1766004	.	mature_transcript_region	2184	2422	.	+	0	ID=BBa_K1766000;Name=BBa_K1766000;Parent=BBa_K1766003
BBa_K1766004	.	sequence_feature	2330	2422	.	+	0	ID=annotation2438244;Name=MicF ;Parent=BBa_K1766000
BBa_K1766004	.	promoter	2068	2175	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_K1766003
BBa_K1766004	.	non_covalent_binding_site	2101	2118	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K1766004	.	non_covalent_binding_site	2080	2097	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K1766004	.	promoter	2165	2170	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K1766004	.	non_covalent_binding_site	2121	2138	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K1766004	.	promoter	2142	2147	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K1766004	.	terminator	2019	2059	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1766004	.	polyA_site	2046	2059	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1766004	.	stem_loop	2026	2045	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1766004	.	engineered_region	2019	2059	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1766004	.	stop_codon	2052	2052	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K1766004
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggcttcctccgaagacgttatc
aaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaag
gtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcct
gtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttc
ccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgc
aagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaac
catgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaa
ctgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctt
acaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaagg
tcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagcagacaca
taaagacaccaaactctcatcaatagttccgtaaatttttattgacagaacttattgacggcagtggcaggtgtcataa
aaaaaaccatgagggtaataaataatgatgaagcgcaatattctggcagtgatcgtccctgcttacttgagtcacacag
aatgcggaaagtacttgatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtga
tgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatt
tgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagat
acccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattttt
caaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaa
ggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatca
tggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagc
agaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatct
gccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatg
gcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatca
tattcgtgttggattattctgcatttttggggagaatggacttactagagactttttaagattattgcggaatggcgaa
ataagcacctaacatcaagcaataataattcaaggttaaaatcaataacttattcttaagtatttgacagcactgaatg
tcaaaacaaaaccttcactcgcaactagaataactcccgctatcatcattaactttatttattaccgtcattcatttct
gaatgtctgtttacccctatttcaaccggatgcctcgcattcggtttttttt