BBa_K1766004
type: ComponentDefinition
persistent identity: BBa K1766004
display id: BBa_K1766004
version: 1
was generated by: iGEM to SBOL conversion
description: OmpR controlled regulatory circuit producing RFP and GFP
created: 2015-09-05T11:00:00Z
modified: 2015-09-06T04:40:47Z
mutable provenance: source
owned by: james
owned by: myers
mutable description: long description
discontinued: false
dominant: false
group u list: _2188_
m user id: 25546
owner id: 25546
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: design
star: false
creator: Sarah Wideman
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1766003
component: BBa_B0010
component: BBa_M30011
component: BBa_K1766002
component: BBa_B0012
sequence annotation: BBa_M30011
sequence annotation: BBa_K1766002
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K1766003
sequence: https://synbiohub.org/public/igem/BBa_K1766004_sequence/1

BBa_K1766003
type: Component
persistent identity: component2445330
display id: component2445330
version: 1
top level: BBa_K1766004
definition: BBa_K1766003
access: public

BBa_B0010
type: Component
persistent identity: component2445318
display id: component2445318
version: 1
top level: BBa_K1766004
definition: BBa_B0010
access: public

BBa_M30011
type: Component
persistent identity: component2445312
display id: component2445312
version: 1
top level: BBa_K1766004
definition: BBa_M30011
access: public

BBa_K1766002
type: Component
persistent identity: component2445321
display id: component2445321
version: 1
top level: BBa_K1766004
definition: BBa_K1766002
access: public

BBa_B0012
type: Component
persistent identity: component2445319
display id: component2445319
version: 1
top level: BBa_K1766004
definition: BBa_B0012
access: public

BBa_M30011
type: SequenceAnnotation
persistent identity: annotation2445312
display id: annotation2445312
version: 1
top level: BBa_K1766004
location: https://synbiohub.org/public/igem/BBa_K1766004/annotation2445312/range2445312/1
component: BBa_M30011

https://synbiohub.org/public/igem/BBa_K1766004/annotation2445312/range2445312/1
type: Range
persistent identity: range2445312
display id: range2445312
version: 1
top level: BBa_K1766004
direction: direction/reverse
start: 1
end: 977
orientation: inline

BBa_K1766002
type: SequenceAnnotation
persistent identity: annotation2445321
display id: annotation2445321
version: 1
top level: BBa_K1766004
location: https://synbiohub.org/public/igem/BBa_K1766004/annotation2445321/range2445321/1
component: BBa_K1766002

https://synbiohub.org/public/igem/BBa_K1766004/annotation2445321/range2445321/1
type: Range
persistent identity: range2445321
display id: range2445321
version: 1
top level: BBa_K1766004
direction: direction/reverse
start: 986
end: 2059
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2445318
display id: annotation2445318
version: 1
top level: BBa_K1766004
location: https://synbiohub.org/public/igem/BBa_K1766004/annotation2445318/range2445318/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1766004/annotation2445318/range2445318/1
type: Range
persistent identity: range2445318
display id: range2445318
version: 1
top level: BBa_K1766004
direction: direction/forward
start: 1939
end: 2010
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2445319
display id: annotation2445319
version: 1
top level: BBa_K1766004
location: https://synbiohub.org/public/igem/BBa_K1766004/annotation2445319/range2445319/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1766004/annotation2445319/range2445319/1
type: Range
persistent identity: range2445319
display id: range2445319
version: 1
top level: BBa_K1766004
direction: direction/forward
start: 2019
end: 2059
orientation: inline

BBa_K1766003
type: SequenceAnnotation
persistent identity: annotation2445330
display id: annotation2445330
version: 1
top level: BBa_K1766004
location: https://synbiohub.org/public/igem/BBa_K1766004/annotation2445330/range2445330/1
component: BBa_K1766003

https://synbiohub.org/public/igem/BBa_K1766004/annotation2445330/range2445330/1
type: Range
persistent identity: range2445330
display id: range2445330
version: 1
top level: BBa_K1766004
direction: direction/reverse
start: 2068
end: 2422
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K1766002
type: ComponentDefinition
persistent identity: BBa K1766002
display id: BBa_K1766002
version: 1
was generated by: iGEM to SBOL conversion
description: MicF regulated GFP
created: 2015-09-05T11:00:00Z
modified: 2015-09-16T11:13:30Z
mutable provenance: e. coli
owned by: james
owned by: myers
mutable description: long description
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2188_
m user id: 25546
owner id: 25546
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: stop codon has to be removed
star: false
creator: Sarah Wideman
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: BBa_J23101
sequence annotation: BBa_K1766001
sequence annotation: MicF target
sequence annotation: OmpF-GFP fusion
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_K1766002_sequence/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2445268
display id: annotation2445268
version: 1
top level: BBa_K1766002
location: https://synbiohub.org/public/igem/BBa_K1766002/annotation2445268/range2445268/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1766002/annotation2445268/range2445268/1
type: Range
persistent identity: range2445268
display id: range2445268
version: 1
top level: BBa_K1766002
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_K1766001
type: SequenceAnnotation
persistent identity: annotation2445267
display id: annotation2445267
version: 1
top level: BBa_K1766002
location: https://synbiohub.org/public/igem/BBa_K1766002/annotation2445267/range2445267/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1766002/annotation2445267/range2445267/1
type: Range
persistent identity: range2445267
display id: range2445267
version: 1
top level: BBa_K1766002
direction: direction/reverse
start: 1
end: 184
orientation: inline

MicF target
type: SequenceAnnotation
persistent identity: annotation2445269
display id: annotation2445269
version: 1
top level: BBa_K1766002
location: https://synbiohub.org/public/igem/BBa_K1766002/annotation2445269/range2445269/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1766002/annotation2445269/range2445269/1
type: Range
persistent identity: range2445269
display id: range2445269
version: 1
top level: BBa_K1766002
direction: direction/forward
start: 36
end: 184
orientation: inline

OmpF-GFP fusion
type: SequenceAnnotation
persistent identity: annotation2445270
display id: annotation2445270
version: 1
top level: BBa_K1766002
location: https://synbiohub.org/public/igem/BBa_K1766002/annotation2445270/range2445270/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1766002/annotation2445270/range2445270/1
type: Range
persistent identity: range2445270
display id: range2445270
version: 1
top level: BBa_K1766002
direction: direction/forward
start: 146
end: 934
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2445271
display id: annotation2445271
version: 1
top level: BBa_K1766002
location: https://synbiohub.org/public/igem/BBa_K1766002/annotation2445271/range2445271/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1766002/annotation2445271/range2445271/1
type: Range
persistent identity: range2445271
display id: range2445271
version: 1
top level: BBa_K1766002
direction: direction/forward
start: 218
end: 934
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2445272
display id: annotation2445272
version: 1
top level: BBa_K1766002
location: https://synbiohub.org/public/igem/BBa_K1766002/annotation2445272/range2445272/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1766002/annotation2445272/range2445272/1
type: Range
persistent identity: range2445272
display id: range2445272
version: 1
top level: BBa_K1766002
direction: direction/forward
start: 954
end: 1025
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2445273
display id: annotation2445273
version: 1
top level: BBa_K1766002
location: https://synbiohub.org/public/igem/BBa_K1766002/annotation2445273/range2445273/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1766002/annotation2445273/range2445273/1
type: Range
persistent identity: range2445273
display id: range2445273
version: 1
top level: BBa_K1766002
direction: direction/forward
start: 1034
end: 1074
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2445274
display id: annotation2445274
version: 1
top level: BBa_K1766002
location: https://synbiohub.org/public/igem/BBa_K1766002/annotation2445274/range2445274/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1766002/annotation2445274/range2445274/1
type: Range
persistent identity: range2445274
display id: range2445274
version: 1
top level: BBa_K1766002
direction: direction/forward
start: 1041
end: 1068
orientation: inline

BBa_K1766003
type: ComponentDefinition
persistent identity: BBa K1766003
display id: BBa_K1766003
version: 1
was generated by: iGEM to SBOL conversion
description: OmpR controlled MicF
created: 2015-09-05T11:00:00Z
modified: 2015-09-06T04:33:57Z
mutable provenance: e. coli
owned by: james
owned by: myers
mutable description: long description
discontinued: false
dominant: false
experience: experience/None
group u list: _2188_
m user id: 25546
owner id: 25546
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: design
star: false
creator: Sarah Wideman
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1766000
component: BBa_R0082
sequence annotation: BBa_R0082
sequence annotation: BBa_K1766000
sequence: https://synbiohub.org/public/igem/BBa_K1766003_sequence/1

BBa_K1766000
type: Component
persistent identity: component2445294
display id: component2445294
version: 1
top level: BBa_K1766003
definition: MicF
access: public

BBa_R0082
type: Component
persistent identity: component2445292
display id: component2445292
version: 1
top level: BBa_K1766003
definition: OmpR
access: public

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2445292
display id: annotation2445292
version: 1
top level: BBa_K1766003
location: https://synbiohub.org/public/igem/BBa_K1766003/annotation2445292/range2445292/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1766003/annotation2445292/range2445292/1
type: Range
persistent identity: range2445292
display id: range2445292
version: 1
top level: BBa_K1766003
direction: direction/reverse
start: 1
end: 108
orientation: inline

BBa_K1766000
type: SequenceAnnotation
persistent identity: annotation2445294
display id: annotation2445294
version: 1
top level: BBa_K1766003
location: https://synbiohub.org/public/igem/BBa_K1766003/annotation2445294/range2445294/1
component: BBa_K1766000

https://synbiohub.org/public/igem/BBa_K1766003/annotation2445294/range2445294/1
type: Range
persistent identity: range2445294
display id: range2445294
version: 1
top level: BBa_K1766003
direction: direction/reverse
start: 117
end: 355
orientation: inline

BBa_M30011
type: ComponentDefinition
persistent identity: BBa M30011
display id: BBa_M30011
version: 1
was generated by: iGEM to SBOL conversion
description: OmpR controlled mRFP
created: 2005-12-14T12:00:00Z
modified: 2015-09-17T06:41:23Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of mRFP (I13507). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. An alternative clone is M30012. Cells bearing this construct are pale pink.
discontinued: false
dominant: true
experience: experience/Works
group u list: _45_1_
m user id: 25546
owner id: 314
owning group id: 1
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: natalie kuldell
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0082
component: BBa_E1010
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0082
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_M30011_sequence/1

BBa_R0082
type: Component
persistent identity: component1768968
display id: component1768968
version: 1
top level: BBa_M30011
definition: OmpR
access: public

BBa_E1010
type: Component
persistent identity: component1768980
display id: component1768980
version: 1
top level: BBa_M30011
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component1768973
display id: component1768973
version: 1
top level: BBa_M30011
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1768996
display id: component1768996
version: 1
top level: BBa_M30011
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1768986
display id: component1768986
version: 1
top level: BBa_M30011
definition: BBa_B0010
access: public

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1768968
display id: annotation1768968
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768968/range1768968/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_M30011/annotation1768968/range1768968/1
type: Range
persistent identity: range1768968
display id: range1768968
version: 1
top level: BBa_M30011
direction: direction/forward
start: 1
end: 108
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1768973
display id: annotation1768973
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768973/range1768973/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30011/annotation1768973/range1768973/1
type: Range
persistent identity: range1768973
display id: range1768973
version: 1
top level: BBa_M30011
direction: direction/forward
start: 117
end: 128
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1768980
display id: annotation1768980
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768980/range1768980/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_M30011/annotation1768980/range1768980/1
type: Range
persistent identity: range1768980
display id: range1768980
version: 1
top level: BBa_M30011
direction: direction/forward
start: 135
end: 815
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1768986
display id: annotation1768986
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768986/range1768986/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30011/annotation1768986/range1768986/1
type: Range
persistent identity: range1768986
display id: range1768986
version: 1
top level: BBa_M30011
direction: direction/forward
start: 849
end: 928
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1768996
display id: annotation1768996
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768996/range1768996/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30011/annotation1768996/range1768996/1
type: Range
persistent identity: range1768996
display id: range1768996
version: 1
top level: BBa_M30011
direction: direction/forward
start: 937
end: 977
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

MicF
type: ComponentDefinition
persistent identity: BBa K1766000
display id: BBa_K1766000
version: 1
was generated by: iGEM to SBOL conversion
description: MicF non-coding regulatory RNA
created: 2015-08-25T11:00:00Z
modified: 2015-09-16T11:12:44Z
mutable provenance: BBa_K1766000 is based on the micF gene found in Escherichia coli. The BioBrick??? was created from a gBlocks?? gene fragment synthesized by Integrated DNA Technologies.
owned by: james
owned by: myers
mutable description: MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5??? untranslated region of ompF mRNA, to prevent translation and promote degradation. MicF can be used together with BBa_K176601 to regulate protein expression.
discontinued: false
dominant: false
experience: experience/None
group u list: _2188_
m user id: 25546
owner id: 25546
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The transcribed MicF only consists of the last 93 nt of BBa_K1766000. The preceding region was added in consideration of the genomic sequence.
star: false
creator: Sarah Wideman
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: MicF
sequence: https://synbiohub.org/public/igem/BBa_K1766000_sequence/1

MicF
type: SequenceAnnotation
persistent identity: annotation2438244
display id: annotation2438244
version: 1
top level: MicF
location: https://synbiohub.org/public/igem/BBa_K1766000/annotation2438244/range2438244/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1766000/annotation2438244/range2438244/1
type: Range
persistent identity: range2438244
display id: range2438244
version: 1
top level: MicF
direction: direction/forward
start: 147
end: 239
orientation: inline

https://synbiohub.org/public/igem/BBa_K1766004_sequence/1
type: Sequence
persistent identity: BBa K1766004 sequence
display id: BBa_K1766004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagcagacacataaagacaccaaactctcatcaatagttccgtaaatttttattgacagaacttattgacggcagtggcaggtgtcataaaaaaaaccatgagggtaataaataatgatgaagcgcaatattctggcagtgatcgtccctgcttacttgagtcacacagaatgcggaaagtacttgatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1766002_sequence/1
type: Sequence
persistent identity: BBa K1766002 sequence
display id: BBa_K1766002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagcagacacataaagacaccaaactctcatcaatagttccgtaaatttttattgacagaacttattgacggcagtggcaggtgtcataaaaaaaaccatgagggtaataaataatgatgaagcgcaatattctggcagtgatcgtccctgcttacttgagtcacacagaatgcggaaagtacttgatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1766003_sequence/1
type: Sequence
persistent identity: BBa K1766003 sequence
display id: BBa_K1766003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagactttttaagattattgcggaatggcgaaataagcacctaacatcaagcaataataattcaaggttaaaatcaataacttattcttaagtatttgacagcactgaatgtcaaaacaaaaccttcactcgcaactagaataactcccgctatcatcattaactttatttattaccgtcattcatttctgaatgtctgtttacccctatttcaaccggatgcctcgcattcggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M30011_sequence/1
type: Sequence
persistent identity: BBa M30011 sequence
display id: BBa_M30011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1766000_sequence/1
type: Sequence
persistent identity: BBa K1766000 sequence
display id: BBa_K1766000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actttttaagattattgcggaatggcgaaataagcacctaacatcaagcaataataattcaaggttaaaatcaataacttattcttaagtatttgacagcactgaatgtcaaaacaaaaccttcactcgcaactagaataactcccgctatcatcattaactttatttattaccgtcattcatttctgaatgtctgtttacccctatttcaaccggatgcctcgcattcggtttttttt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z