BBa_K1766008
1
BBa_K1766008
EnvZ_V5 osmoregulatory histidine kinase from <i>E.coli</i>.
2015-09-12T11:00:00Z
2015-09-16T01:50:44Z
EnvZ was isolated from the <i>E.coli</i> genome by ROGER??.
EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in <i>E.coli</i>. Structure includes a C-terminal V5-tag through a GGSSAAG linker. The part was used in a pRD400 backbone behind a lac-inducible promoter to study signaling as well as expression.
false
false
_2188_
25315
25624
9
false
Sequence includes illegal restirction site EcorI at 315-321 bp. Removed through site-directed mutagenesis exchanging A317G .
false
Pontus H??jer
annotation2453183
1
V5 tag
range2453183
1
1372
1416
annotation2453181
1
EnvZ
range2453181
1
1
1350
annotation2453182
1
GGSSAAG linker
range2453182
1
1351
1371
BBa_K1766008_sequence
1
atgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggggcggcagcagcgcggcgggcggcaaaccgattccgaacccgctgctgggcctggatagcacctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z