BBa_K1766009 1 BBa_K1766009 BAR construct 1 2015-09-12T11:00:00Z 2015-09-18T10:11:51Z Part created through overlap PCR using EnvZ_V5 (<a href="http://parts.igem.org/Part:BBa_K1766008">BBa_K1766008</a>) and Z:HER2:342. BAR construct 1 was created in the hope of making a receptor able to signal binding to an antigen. By making a chimera between a histidine kinase (EnvZ) and a affibody molecule (Z:HER2:342) we would have a receptor capable of detecting the HER2 (human epidermal growth factor receptor) protein biomarker, commonly used for detecting breast cancer. The construct would use the EnvZ-OmpR two-component regulatory system to signal binding and activation of the histidine kinase. However successful signaling has not been documented. The construct has been studied for expression, pressence in membrane and affinity towards HER2. false false _2188_ 25624 25624 9 false The bacterial antigen receptor (BAR), construct 1, was created by replacing part of the periplasmic domain of EnvZ. Structure prediction of the periplasmic domain of EnvZ revealed a possible PAS domain with a Beta-sheet->Coil->Alpha-helix->Coil structure. This structure was replaced with the Z:HER2:342 affibody with documented affinity towards the human epidermal growth factor receptor (HER2) (REFERENCE). false Pontus H??jer annotation2453191 1 Z:HER2:342 range2453191 1 256 429 annotation2453189 1 GGSSAAG linker range2453189 1 1342 1363 annotation2453190 1 V5 tag range2453190 1 1364 1404 annotation2453188 1 BAR construct 1 range2453188 1 1 1341 BBa_K1766009_sequence 1 atgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctgggggtagacaacaaattcaacaaagaaatgcgaaacgcgtattgggagatcgctctgttacctaacttaaacaatcagcaaaagcgcgccttcatccgcagtttatatgatgacccaagccaaagcgctaacttgctagcagaagctaaaaagctaaatgatgctcaggcgccgaaagtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggggcggcagcagcgcggcgggcggcaaaccgattccgaacccgctgctgggcctggatagcacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z