BBa_K1766010
1
BBa_K1766010
BAR construct 2
2015-09-13T11:00:00Z
2015-09-16T01:43:51Z
Part created through overlap PCR using EnvZ_V5 (<a href="http://parts.igem.org/Part:BBa_K1766008">BBa_K1766008</a>) and Z:HER2:342.
The bacterial antigen receptor (BAR) was created in the hope of making a receptor able to signal binding to an antigen. By making a chimera between a histidine kinase (EnvZ) and a affibody molecule (Z:HER2:342) we would have a receptor capable of detecting the HER2 (human epidermal growth factor receptor) protein biomarker, commonly used for detecting breast cancer. The construct would use the EnvZ-OmpR two-component regulatory system to signal binding and activation of the histidine kinase. However successful function of the receptor has not been fully documented.
The construct has been studied for expression, pressence in membrane and affinity towards HER2.
false
false
_2188_
25315
25624
9
false
The bacterial antigen receptor (BAR), construct 1, was created by
false
Pontus H??jer
annotation2454736
1
Z:HER2:342
range2454736
1
256
429
annotation2454740
1
GSGGGGSG linker
range2454740
1
430
453
annotation2454630
1
GGSSAAG linker
range2454630
1
1366
1387
annotation2454599
1
BAR construct 2
range2454599
1
1
1365
annotation2454734
1
V5 tag
range2454734
1
1388
1431
BBa_K1766010_sequence
1
atgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctgggggtagacaacaaattcaacaaagaaatgcgaaacgcgtattgggagatcgctctgttacctaacttaaacaatcagcaaaagcgcgccttcatccgcagtttatatgatgacccaagccaaagcgctaacttgctagcagaagctaaaaagctaaatgatgctcaggcgccgaaaggttctggtggtggtggcagtggtgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggggcggcagcagcgcggcgggcggcaaaccgattccgaacccgctgctgggcctggatagcacctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z