BBa_K1766013 1 BBa_K1766013 EnvZ_V5 osmoregulatory histidine kinase from <i>E.coli</i>. 2015-09-13T11:00:00Z 2015-09-16T01:46:00Z EnvZ was isolated from <i>E.coli</i> K12. EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in <i>E.coli</i>. Structure includes the EnvZ247 allele [1] which is linked through a GGSSAAG linker to a V5 epitope tag. The full structure has a predicted size of 115.36 kDa [2]. The part was provided in the pRD400 (or pEnvZ) backbone under a lac-inducible promoter [3]. <br /> Two-component regulatory systems are a common in procaryotes for signal transduction through the membrane. In this case, EnvZ is activated by changes in osmotic pressure leading to phosphorylation of the kinase. The response regulator OmpR is then activated throungh phosphorylation resultning in the regulation of expression of the outer membrane porin proteins OmpF and OmpC. false false _2188_ 25315 25624 9 false Illegal restirction site EcorI present in original sequence at 315-321 bp. Site-directed mutagenesis, exchanging A317G, was appempted using primers F1 and R1 but was unsuccesful. false Pontus H??jer annotation2454815 1 GGSSAAG linker range2454815 1 1351 1372 annotation2454814 1 EnvZ range2454814 1 1 1350 annotation2454816 1 V5 tag range2454816 1 1373 1413 BBa_K1766013_sequence 1 atgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggggcggcagcagcgcggcgggcggcaaaccgattccgaacccgctgctgggcctggatagcacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z