LOCUS BBa_K1767003 3597 bp DNA linear 22-Dec-2024 DEFINITION P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter ACCESSION BBa_K1767003 VERSION BBa_K1767003.1 FEATURES Location/Qualifiers promoter 1..37 /label=BBa_I14032/p(Lac) IQ misc_feature 3..8 /label=-35 misc_feature 26..31 /label=-10 RBS 44..50 /label=RBS/BBa_B0034 CDS 59..812 /label=AiiA RBS 820..832 /label=RBS CDS 838..1590 /label=LuxR stem_loop 1627..1707 /label=BBa_B0010/term stem_loop 1715..1756 /label=BBa_B0012/term promoter 1764..1818 /label=BBa_R0062/pLux promoter misc_feature 1..1818 /label=BBa_K1767001 misc_feature 1831..1834 /label=conserved RBS 1827..1838 /label=BBa_B0034 CDS 1848..2464 /label=tetR misc_feature 2465..2498 /label=SsrA misc_feature 2505..2529 /label=Help:Barcodes CDS 1845..2504 /label=BBa_C0040 stem_loop 2549..2592 /label=stem_loop misc_feature 2538..2617 /label=BBa_B0010 terminator 2538..2617 /label=BBa_B0010 stem_loop 2633..2652 /label=T7 TE misc_feature 2659..2659 /label=stop polyA_site 2653..2666 /label=polya misc_feature 2626..2666 /label=BBa_B0012 terminator 2626..2666 /label=BBa_B0012 misc_feature 1827..2666 /label=BBa_P0440 misc_feature 1..2666 /label=BBa_K1767002 misc_feature 2675..2693 /label=TetR 1 misc_feature 2675..2728 /label=BBa_R0040 promoter 2694..2699 /label=-35 misc_feature 2700..2718 /label=TetR 2 promoter 2717..2722 /label=-10 promoter 2675..2728 /label=BBa_R0040 misc_feature 2741..2744 /label=conserved RBS 2737..2748 /label=BBa_B0034 CDS 2755..3429 /label=mrfp1 misc_feature 3436..3460 /label=Help:Barcodes CDS 2755..3435 /label=BBa_E1010 stem_loop 3480..3523 /label=stem_loop misc_feature 3469..3548 /label=BBa_B0010 terminator 3469..3548 /label=BBa_B0010 stem_loop 3564..3583 /label=T7 TE misc_feature 3590..3590 /label=stop polyA_site 3584..3597 /label=polya misc_feature 3557..3597 /label=BBa_B0012 terminator 3557..3597 /label=BBa_B0012 misc_feature 2675..3597 /label=BBa_I13521 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac aaagagggga catactagat 61 gacagtaaag aagctttatt tcgtcccagc aggtcgttgt atgttggatc attcgtctgt 121 taatagtaca ttaacaccag gagaattatt agacttaccg gtttggtgtt atcttttgga 181 gactgaagaa ggacctattt tagtagatac aggtatgcca gaaagtgcag ttaataatga 241 aggtcttttt aacggtacat ttgtcgaagg gcaggtttta ccgaaaatga ctgaagaaga 301 tagaatcgtg aatattttaa aacgggttgg ttatgagccg gaagaccttc tttatattat 361 tagttctcac ttgcattttg atcatgcagg aggaaatggc gcttttataa atacaccaat 421 cattgtacag cgtgctgaat atgaggcggc gcagcatagc gaagaatatt tgaaagaatg 481 tatattgccg aatttaaact acaaaatcat tgaaggtgat tatgaagtcg taccaggagt 541 tcaattattg catacaccag gccatactcc agggcatcaa tcgctattaa ttgagacaga 601 aaaatccggt cctgtattat taacgattga tgcatcgtat acgaaagaga attttgaaaa 661 tgaagtgcca tttgcgggat ttgattcaga attagcttta tcttcaatta aacgtttaaa 721 agaagtggtg atgaaagaga agccgattgt tttctttgga catgatatag agcaggaaag 781 gggatgtaaa gtgttccctg aatatatata ataatagaga aagaggagaa atactagatg 841 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 901 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 961 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 1021 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 1081 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 1141 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 1201 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 1261 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 1321 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 1381 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 1441 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 1501 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 1561 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 1621 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1681 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1741 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1801 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgtcc agattagata 1861 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa 1921 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa 1981 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc 2041 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt 2101 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta 2161 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt 2221 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat 2281 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta 2341 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct 2401 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg 2461 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt 2521 gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 2581 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 2641 ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat 2701 ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata ctagatggct 2761 tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt 2821 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag 2881 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc 2941 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac 3001 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt 3061 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt 3121 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt 3181 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa 3241 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg 3301 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc 3361 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc 3421 accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa 3481 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 3541 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //