##gff-version 3
##sequence-region BBa_K1767003 1 3597
BBa_K1767003	.	engineered_region	2675	3597	.	+	0	ID=BBa_I13521;Name=BBa_I13521
BBa_K1767003	.	CDS	2755	3435	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K1767003	.	engineered_tag	3436	3460	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1767003	.	CDS	2755	3429	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1767003	.	terminator	3469	3548	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K1767003	.	engineered_region	3469	3548	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1767003	.	stem_loop	3480	3523	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1767003	.	ribosome_entry_site	2737	2748	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K1767003	.	conserved_region	2741	2744	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1767003	.	terminator	3557	3597	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K1767003	.	stop_codon	3590	3590	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1767003	.	polyA_site	3584	3597	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1767003	.	stem_loop	3564	3583	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1767003	.	engineered_region	3557	3597	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1767003	.	promoter	2675	2728	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K1767003	.	engineered_region	2675	2728	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1767003	.	promoter	2694	2699	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1767003	.	promoter	2717	2722	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1767003	.	non_covalent_binding_site	2675	2693	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1767003	.	non_covalent_binding_site	2700	2718	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1767003	.	engineered_region	1	2666	.	+	0	ID=BBa_K1767002;Name=BBa_K1767002
BBa_K1767003	.	engineered_region	1	1818	.	+	0	ID=BBa_K1767001;Name=BBa_K1767001;Parent=BBa_K1767002
BBa_K1767003	.	ribosome_entry_site	44	50	.	+	0	ID=annotation2458931;Name=RBS/BBa_B0034;Parent=BBa_K1767001
BBa_K1767003	.	ribosome_entry_site	820	832	.	+	0	ID=annotation2458933;Name=RBS;Parent=BBa_K1767001
BBa_K1767003	.	sequence_feature	26	31	.	+	0	ID=annotation2458930;Name=-10;Parent=BBa_K1767001
BBa_K1767003	.	sequence_feature	3	8	.	+	0	ID=annotation2458929;Name=-35;Parent=BBa_K1767001
BBa_K1767003	.	promoter	1764	1818	.	+	0	ID=annotation2458937;Name=BBa_R0062/pLux promoter;Parent=BBa_K1767001
BBa_K1767003	.	promoter	1	37	.	+	0	ID=annotation2458928;Name=BBa_I14032/p(Lac) IQ;Parent=BBa_K1767001
BBa_K1767003	.	stem_loop	1627	1707	.	+	0	ID=annotation2458935;Name=BBa_B0010/term;Parent=BBa_K1767001
BBa_K1767003	.	CDS	838	1590	.	+	0	ID=annotation2458934;Name=LuxR;Parent=BBa_K1767001
BBa_K1767003	.	stem_loop	1715	1756	.	+	0	ID=annotation2458936;Name=BBa_B0012/term;Parent=BBa_K1767001
BBa_K1767003	.	CDS	59	812	.	+	0	ID=annotation2458932;Name=AiiA;Parent=BBa_K1767001
BBa_K1767003	.	engineered_region	1827	2666	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K1767002
BBa_K1767003	.	terminator	2626	2666	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1767003	.	stop_codon	2659	2659	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1767003	.	polyA_site	2653	2666	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1767003	.	stem_loop	2633	2652	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1767003	.	engineered_region	2626	2666	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1767003	.	terminator	2538	2617	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1767003	.	engineered_region	2538	2617	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1767003	.	stem_loop	2549	2592	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1767003	.	CDS	1845	2504	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1767003	.	engineered_tag	2505	2529	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1767003	.	CDS	1848	2464	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1767003	.	tag	2465	2498	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1767003	.	ribosome_entry_site	1827	1838	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1767003	.	conserved_region	1831	1834	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1767003
tggtgcaaaacctttcgcggtatggcatgatagcgcctacaaagaggggacatactagatgacagtaaagaagctttat
ttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttac
cggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataa
tgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatatt
ttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaa
atggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaa
agaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcat
acaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatg
catcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaa
acgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaa
gtgttccctgaatatatataataatagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacag
aataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacat
tgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattacccta
aaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattc
accaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatca
ggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaa
aagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataatta
tcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgc
gaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgc
aaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactt
taaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataa
atactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgctta
atgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggca
tgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgcccttta
gaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggag
caaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaaca
aggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaa
gagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaat
tatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaa
atgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatca
ctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtg
atagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata