LOCUS BBa_K1767005 2823 bp DNA linear 29-Nov-2024 DEFINITION P(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter ACCESSION BBa_K1767005 VERSION BBa_K1767005.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 misc_feature 1057..1060 /label=conserved RBS 1053..1064 /label=BBa_B0034 CDS 1074..1690 /label=tetR misc_feature 1691..1724 /label=SsrA misc_feature 1731..1755 /label=Help:Barcodes CDS 1071..1730 /label=BBa_C0040 stem_loop 1775..1818 /label=stem_loop misc_feature 1764..1843 /label=BBa_B0010 terminator 1764..1843 /label=BBa_B0010 stem_loop 1859..1878 /label=T7 TE misc_feature 1885..1885 /label=stop polyA_site 1879..1892 /label=polya misc_feature 1852..1892 /label=BBa_B0012 terminator 1852..1892 /label=BBa_B0012 misc_feature 1053..1892 /label=BBa_P0440 misc_feature 1..1892 /label=BBa_K1767004 misc_feature 1901..1919 /label=TetR 1 misc_feature 1901..1954 /label=BBa_R0040 promoter 1920..1925 /label=-35 misc_feature 1926..1944 /label=TetR 2 promoter 1943..1948 /label=-10 promoter 1901..1954 /label=BBa_R0040 misc_feature 1967..1970 /label=conserved RBS 1963..1974 /label=BBa_B0034 CDS 1981..2655 /label=mrfp1 misc_feature 2662..2686 /label=Help:Barcodes CDS 1981..2661 /label=BBa_E1010 stem_loop 2706..2749 /label=stem_loop misc_feature 2695..2774 /label=BBa_B0010 terminator 2695..2774 /label=BBa_B0010 stem_loop 2790..2809 /label=T7 TE misc_feature 2816..2816 /label=stop polyA_site 2810..2823 /label=polya misc_feature 2783..2823 /label=BBa_B0012 terminator 2783..2823 /label=BBa_B0012 misc_feature 1901..2823 /label=BBa_I13521 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agaaagagga gaaatactag atgtccagat 1081 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag 1141 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc 1201 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc 1261 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta 1321 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac 1381 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag 1441 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt 1501 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg 1561 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc 1621 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg 1681 aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt 1741 gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 1801 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 1861 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat 1921 tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag 1981 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt 2041 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt 2101 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc 2161 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg 2221 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa 2281 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac 2341 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc 2401 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa 2461 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc 2521 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac 2581 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt 2641 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc 2701 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 2761 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2821 ata //