BBa_K1767009
type: ComponentDefinition
persistent identity: BBa K1767009
display id: BBa_K1767009
version: 1
was generated by: iGEM to SBOL conversion
description: P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS eyfp ter ter
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T08:57:51Z
mutable provenance: iGEM registry
owned by: james
owned by: myers
mutable description: We designed and built a kill switch mechanism that based on the activation of a lethal gene downstream of an inducible promoter that can be activated over time. In order to understand our system we started to test the first part of our genetic circuit , to test it we built the first part ( k1767001 ) driving expression of florescence protein . The here presented BioBrick consists of a constitutive promoter(I14032) driving expression of LuxR followed by a Lux promoter (R0062) , driving expression of YFP. The idea is to retrieve information about the expression of a fluorescent reporter downstream of the pLuxR promoter in absence and increasing concentrations of AHL. The scheme below explains how this BioBrick works :
discontinued: false
dominant: false
group u list: _2189_
m user id: 25573
owner id: 25573
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: naseeb saida
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I13003
component: BBa_K1767001
sequence annotation: BBa_K1767001
sequence annotation: BBa_I13003
sequence: https://synbiohub.org/public/igem/BBa_K1767009_sequence/1

BBa_I13003
type: Component
persistent identity: component2460919
display id: component2460919
version: 1
top level: BBa_K1767009
definition: BBa_I13003
access: public

BBa_K1767001
type: Component
persistent identity: component2460908
display id: component2460908
version: 1
top level: BBa_K1767009
definition: BBa_K1767001
access: public

BBa_K1767001
type: SequenceAnnotation
persistent identity: annotation2460908
display id: annotation2460908
version: 1
top level: BBa_K1767009
location: https://synbiohub.org/public/igem/BBa_K1767009/annotation2460908/range2460908/1
component: BBa_K1767001

https://synbiohub.org/public/igem/BBa_K1767009/annotation2460908/range2460908/1
type: Range
persistent identity: range2460908
display id: range2460908
version: 1
top level: BBa_K1767009
direction: direction/reverse
start: 1
end: 1818
orientation: inline

BBa_I13003
type: SequenceAnnotation
persistent identity: annotation2460919
display id: annotation2460919
version: 1
top level: BBa_K1767009
location: https://synbiohub.org/public/igem/BBa_K1767009/annotation2460919/range2460919/1
component: BBa_I13003

https://synbiohub.org/public/igem/BBa_K1767009/annotation2460919/range2460919/1
type: Range
persistent identity: range2460919
display id: range2460919
version: 1
top level: BBa_K1767009
direction: direction/reverse
start: 1827
end: 2705
orientation: inline

BBa_K1767001
type: ComponentDefinition
persistent identity: BBa K1767001
display id: BBa_K1767001
version: 1
was generated by: iGEM to SBOL conversion
description: P(Lac)IQ RBS Aiia RBS LuxR term term luxpR
created: 2015-09-14T11:00:00Z
modified: 2015-09-17T02:05:48Z
mutable provenance: parts registry and gBlocks ordered from idt
owned by: james
owned by: myers
mutable description: We designed and built a kill switch mechanism that is based on the activation of a lethal gene downstream of an inducible promoter that is activated over time. The presented part is the first module of our designed genetic circuit (BBa_K1767003). In order to characterize and understand each module of the genetic circuit we designed two composite parts that allow us to verify the (1) the degradation rate of AHL by AiiA and (2) induction of the pLux promoter by LuxR in presence of AHL with and without co-expression of AiiA. (1) The here presented BioBrick consists of a constitutive promoter driving expression of AiiA and LuxR followed by a Lux promoter which drives expression of a fluorescent reporter (here: yellow fluorescent protein). For a schematic overview see the figure below
discontinued: false
dominant: false
experience: experience/None
group u list: _2189_
m user id: 6733
owner id: 6733
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none
star: false
creator: iGEM Technion HS
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_I14032/p(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence annotation: RBS/BBa_B0034
sequence annotation: AiiA
sequence annotation: RBS
sequence annotation: LuxR
sequence annotation: BBa_B0010/term
sequence annotation: BBa_B0012/term
sequence annotation: BBa_R0062/pLux promoter
sequence: https://synbiohub.org/public/igem/BBa_K1767001_sequence/1

BBa_I14032/p(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation2458928
display id: annotation2458928
version: 1
top level: BBa_K1767001
location: https://synbiohub.org/public/igem/BBa_K1767001/annotation2458928/range2458928/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1767001/annotation2458928/range2458928/1
type: Range
persistent identity: range2458928
display id: range2458928
version: 1
top level: BBa_K1767001
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2458929
display id: annotation2458929
version: 1
top level: BBa_K1767001
location: https://synbiohub.org/public/igem/BBa_K1767001/annotation2458929/range2458929/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1767001/annotation2458929/range2458929/1
type: Range
persistent identity: range2458929
display id: range2458929
version: 1
top level: BBa_K1767001
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2458930
display id: annotation2458930
version: 1
top level: BBa_K1767001
location: https://synbiohub.org/public/igem/BBa_K1767001/annotation2458930/range2458930/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1767001/annotation2458930/range2458930/1
type: Range
persistent identity: range2458930
display id: range2458930
version: 1
top level: BBa_K1767001
direction: direction/forward
start: 26
end: 31
orientation: inline

RBS/BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2458931
display id: annotation2458931
version: 1
top level: BBa_K1767001
location: https://synbiohub.org/public/igem/BBa_K1767001/annotation2458931/range2458931/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1767001/annotation2458931/range2458931/1
type: Range
persistent identity: range2458931
display id: range2458931
version: 1
top level: BBa_K1767001
direction: direction/forward
start: 44
end: 50
orientation: inline

AiiA
type: SequenceAnnotation
persistent identity: annotation2458932
display id: annotation2458932
version: 1
top level: BBa_K1767001
location: https://synbiohub.org/public/igem/BBa_K1767001/annotation2458932/range2458932/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1767001/annotation2458932/range2458932/1
type: Range
persistent identity: range2458932
display id: range2458932
version: 1
top level: BBa_K1767001
direction: direction/forward
start: 59
end: 812
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2458933
display id: annotation2458933
version: 1
top level: BBa_K1767001
location: https://synbiohub.org/public/igem/BBa_K1767001/annotation2458933/range2458933/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1767001/annotation2458933/range2458933/1
type: Range
persistent identity: range2458933
display id: range2458933
version: 1
top level: BBa_K1767001
direction: direction/forward
start: 820
end: 832
orientation: inline

LuxR
type: SequenceAnnotation
persistent identity: annotation2458934
display id: annotation2458934
version: 1
top level: BBa_K1767001
location: https://synbiohub.org/public/igem/BBa_K1767001/annotation2458934/range2458934/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1767001/annotation2458934/range2458934/1
type: Range
persistent identity: range2458934
display id: range2458934
version: 1
top level: BBa_K1767001
direction: direction/forward
start: 838
end: 1590
orientation: inline

BBa_B0010/term
type: SequenceAnnotation
persistent identity: annotation2458935
display id: annotation2458935
version: 1
top level: BBa_K1767001
location: https://synbiohub.org/public/igem/BBa_K1767001/annotation2458935/range2458935/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1767001/annotation2458935/range2458935/1
type: Range
persistent identity: range2458935
display id: range2458935
version: 1
top level: BBa_K1767001
direction: direction/forward
start: 1627
end: 1707
orientation: inline

BBa_B0012/term
type: SequenceAnnotation
persistent identity: annotation2458936
display id: annotation2458936
version: 1
top level: BBa_K1767001
location: https://synbiohub.org/public/igem/BBa_K1767001/annotation2458936/range2458936/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1767001/annotation2458936/range2458936/1
type: Range
persistent identity: range2458936
display id: range2458936
version: 1
top level: BBa_K1767001
direction: direction/forward
start: 1715
end: 1756
orientation: inline

BBa_R0062/pLux promoter
type: SequenceAnnotation
persistent identity: annotation2458937
display id: annotation2458937
version: 1
top level: BBa_K1767001
location: https://synbiohub.org/public/igem/BBa_K1767001/annotation2458937/range2458937/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1767001/annotation2458937/range2458937/1
type: Range
persistent identity: range2458937
display id: range2458937
version: 1
top level: BBa_K1767001
direction: direction/forward
start: 1764
end: 1818
orientation: inline

BBa_I13003
type: ComponentDefinition
persistent identity: BBa I13003
display id: BBa_I13003
version: 1
was generated by: iGEM to SBOL conversion
description: medium RBS, YFP (-LVA), double terminator
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0012
component: BBa_B0032
component: BBa_B0010
component: BBa_E0030
sequence annotation: BBa_B0032
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13003_sequence/1

BBa_B0012
type: Component
persistent identity: component943495
display id: component943495
version: 1
top level: BBa_I13003
definition: BBa_B0012
access: public

BBa_B0032
type: Component
persistent identity: component943477
display id: component943477
version: 1
top level: BBa_I13003
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component943485
display id: component943485
version: 1
top level: BBa_I13003
definition: BBa_B0010
access: public

BBa_E0030
type: Component
persistent identity: component943480
display id: component943480
version: 1
top level: BBa_I13003
definition: eyfp
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation943477
display id: annotation943477
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943477/range943477/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13003/annotation943477/range943477/1
type: Range
persistent identity: range943477
display id: range943477
version: 1
top level: BBa_I13003
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943480
display id: annotation943480
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943480/range943480/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13003/annotation943480/range943480/1
type: Range
persistent identity: range943480
display id: range943480
version: 1
top level: BBa_I13003
direction: direction/forward
start: 20
end: 742
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943485
display id: annotation943485
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943485/range943485/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13003/annotation943485/range943485/1
type: Range
persistent identity: range943485
display id: range943485
version: 1
top level: BBa_I13003
direction: direction/forward
start: 751
end: 830
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943495
display id: annotation943495
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943495/range943495/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13003/annotation943495/range943495/1
type: Range
persistent identity: range943495
display id: range943495
version: 1
top level: BBa_I13003
direction: direction/forward
start: 839
end: 879
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1767009_sequence/1
type: Sequence
persistent identity: BBa K1767009 sequence
display id: BBa_K1767009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctacaaagaggggacatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatataataatagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1767001_sequence/1
type: Sequence
persistent identity: BBa K1767001 sequence
display id: BBa_K1767001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctacaaagaggggacatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatataataatagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13003_sequence/1
type: Sequence
persistent identity: BBa I13003 sequence
display id: BBa_I13003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z