LOCUS BBa_K1767010 1931 bp DNA linear 22-Nov-2024 DEFINITION P(Lac)IQ RBS LuxR ter ter luxpR RBS eyfp ter ter ACCESSION BBa_K1767010 VERSION BBa_K1767010.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 RBS 1053..1065 /label=RBS-3\Weak RBS 1059..1062 /label=RBS misc_feature 1053..1065 /label=BBa_B0032 RBS 1053..1065 /label=BBa_B0032 CDS 1072..1794 /label=BBa_E0030 stem_loop 1814..1857 /label=stem_loop misc_feature 1803..1882 /label=BBa_B0010 terminator 1803..1882 /label=BBa_B0010 stem_loop 1898..1917 /label=T7 TE misc_feature 1924..1924 /label=stop polyA_site 1918..1931 /label=polya misc_feature 1891..1931 /label=BBa_B0012 terminator 1891..1931 /label=BBa_B0012 misc_feature 1053..1931 /label=BBa_I13003 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agtcacacag gaaagtacta gatggtgagc 1081 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 1141 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 1201 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 1261 accttcggct acggcctgca atgcttcgcc cgctaccccg accacatgaa gctgcacgac 1321 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 1381 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 1441 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 1501 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 1561 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 1621 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 1681 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 1741 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag 1801 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 1861 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 1921 ctgcgtttat a //