BBa_K1768001
1
BBa_K1768001
Recombinase invertible constitutive promoter 1
2015-09-11T11:00:00Z
2015-09-18T06:29:26Z
Lox66: BBa_I718016
Lox71: BBa_J23100
Constitutive promoter: BBa_J23100
A constitutive promoter flanked by Lox71 and Lox66 which is recognised by the Cre enzyme which initiates a single round of recombination between these sites. Thus the direction of the promoter is changed allowing expression of a different gene.
false
false
_2190_
26979
26979
9
false
Lox71 is reserved relative to Lox66.
false
Gert Pietersen
BBa_K1768005
1
BBa_K1768005
Invertible promoter with RFP reporter
2015-09-17T11:00:00Z
2015-09-25T07:45:33Z
BBa_K1768001
BBa_B0034
BBa_E1010
This part was synthesised by IDT
This part is for the characterisation of the Invertible promoter BBa_K1768001.
false
false
_2190_
26979
26979
9
false
NA
false
Gert Pietersen
component2471272
1
BBa_K1768001
component2471277
1
BBa_E1010
component2471274
1
BBa_B0034
annotation2471274
1
BBa_B0034
range2471274
1
272
283
annotation2471272
1
BBa_K1768001
range2471272
1
1
263
annotation2471277
1
BBa_E1010
range2471277
1
290
995
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_E1010
1
mRFP1
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
2004-07-27T11:00:00Z
2015-08-31T04:07:26Z
Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a>
Released HQ 2013
monomeric RFP:
Red Fluorescent Protein.
Excitation peak: 584 nm
Emission peak: 607 nm
false
false
_11_1_
0
52
7
In stock
false
TAATAA double stop codon added (DE).
Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
true
Drew Endy
annotation2214014
1
Help:Barcodes
range2214014
1
682
706
annotation1014044
1
mrfp1
range1014044
1
1
675
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1768001_sequence
1
ataacttggtatagcatacattatacgaacggtactgggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgctagcactgtacctaggactgagctagccgtcaagtcagccagtttagtctgaccatctcatctgtaacaacattggcaacgctacctttgccatgtttcagaaacaactctggtattgaagcatattacatacgatatgcttgccat
BBa_E1010_sequence
1
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
BBa_K1768005_sequence
1
ataacttggtatagcatacattatacgaacggtactgggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgctagcactgtacctaggactgagctagccgtcaagtcagccagtttagtctgaccatctcatctgtaacaacattggcaacgctacctttgccatgtttcagaaacaactctggtattgaagcatattacatacgatatgcttgccattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z