LOCUS       BBa_K1769003            2154 bp    DNA     linear       29-Nov-2024
DEFINITION  Salicylic acid sensor
ACCESSION   BBa_K1769003
VERSION     BBa_K1769003.1  
FEATURES             Location/Qualifiers
     promoter        1..1268
                     /label=BBa_J61051
     RBS             1277..1291
                     /label=RBS-1\Strong
     RBS             1284..1288
                     /label=RBS
     misc_feature    1277..1291
                     /label=BBa_B0030
     RBS             1277..1291
                     /label=BBa_B0030
     CDS             1298..2017
                     /label=GFP protein
     CDS             1298..2017
                     /label=BBa_E0040
     stem_loop       2037..2080
                     /label=stem_loop
     misc_feature    2026..2105
                     /label=BBa_B0010
     terminator      2026..2105
                     /label=BBa_B0010
     stem_loop       2121..2140
                     /label=T7 TE
     misc_feature    2147..2147
                     /label=stop
     polyA_site      2141..2154
                     /label=polya
     misc_feature    2114..2154
                     /label=BBa_B0012
     terminator      2114..2154
                     /label=BBa_B0012
     misc_feature    1277..2154
                     /label=BBa_E0840
ORIGIN
        1 ggccgctgcg atcccgcgaa gaaccaaaaa agctcgacag agggcgcggt cattttaggt
       61 cgggcggatc ggcgccgccg gctcggctgg tgtgccgcac agcaccgcct acgtgagctg
      121 ccagttgatg aacttccccc gttgccagct agggcgcaag cgggctgtat aagatcactg
      181 cccatcacat tgatcggctc ggattttttc tcaatccgta aacaggtcaa acatcagttg
      241 ccgcaaccaa atattggcta ggtccttgtg gtacttcgca tgccagaaca tgttgatggc
      301 tatttcaggc aagacgactg ggtgcggcaa ggcgcttagg ccgaagggct ccacgcagca
      361 gtcggctaaa cgtatcggca cagtggcgag cagatcggtg cgctggagga tgtggccaac
      421 ggcggcgaag tgcggcactt ccagacggat gtcgcgccgg atgccgaccc gtgtcatgta
      481 cgtgtccacc tcgccgtggc cggtgccagc ggcgatgaca cgcacgtggc cgtaggaaca
      541 gaagcgctcc agagtcaggg gttcgcgggt gactggatgg tccttgcgac ataggcacac
      601 gtagtgattc tggagcagcc ggcgctgaaa gaagccagtt tgcagattgg gaagcaggcc
      661 cacggccaag tccacggttc cgttctgcaa ggcctgcatc aggctcatcg aactgtcgcg
      721 caccgtactg atcacgcaat tgggggcctg gtgagccagc acatccatca gccgcggcat
      781 gaagtagatc tcgccaatgt cggtcatggc cagggtgaag gtacgctcgc tggtcagcgg
      841 atcgaagctt tcatggtgct gtagggcgtt gcgcagtgcg tgcatggccg aagtgacggg
      901 ctcggccaga tgcgcggcat agggtgtggg ttccattccc tgatgtgtgc gcacgaagag
      961 tgggtcctgt agcgaggtgc gcaggcgttt cagcgcattg ctcacggcag gctgggtcag
     1021 gcccaggttc tccgcagtga tagagacgcg tctgtcgacc agcaactggt tgaacaccac
     1081 cagcaggttt aaatccaggt cacgcagttc catggggcct cgcttgggtt attgctggtg
     1141 cccggccggg cgcaatattc atgttgatga tttattatat atcgagtggt gtatttatca
     1201 atattgtttg ctccgttatc gttattaaca agtcatcaat aaagccatca cgagtaccat
     1261 agaggatcta ctagagatta aagaggagaa atactagatg cgtaaaggag aagaactttt
     1321 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     1381 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
     1441 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt
     1501 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat
     1561 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
     1621 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
     1681 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
     1741 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
     1801 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
     1861 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
     1921 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
     1981 gattacacat ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa
     2041 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2101 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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