LOCUS BBa_K1769010 4301 bp DNA linear 22-Dec-2024 DEFINITION J61051+RBS+LuxR+Ter+pLUX+RBS+LuxI+Ter+pLux+RBS+Aiia+Ter+pLux+RBS+Te r ACCESSION BBa_K1769010 VERSION BBa_K1769010.1 FEATURES Location/Qualifiers promoter 1..1268 /label=BBa_J61051 misc_feature 1281..1284 /label=conserved RBS 1277..1288 /label=BBa_B0034 misc_feature 1295..1296 /label=prefix misc_feature 1468..1468 /label=T misc_feature 1786..1786 /label=A CDS 1295..2044 /label=luxR misc_feature 1295..2050 /label=BBa_C0062 misc_feature 2051..2075 /label=Help:Barcodes CDS 1295..2050 /label=BBa_C0062 stem_loop 2095..2138 /label=stem_loop misc_feature 2084..2163 /label=BBa_B0010 terminator 2084..2163 /label=BBa_B0010 stem_loop 2179..2198 /label=T7 TE misc_feature 2205..2205 /label=stop polyA_site 2199..2212 /label=polya misc_feature 2172..2212 /label=BBa_B0012 terminator 2172..2212 /label=BBa_B0012 terminator 2084..2212 /label=BBa_B0015 misc_feature 2221..2240 /label=LuxR/HSL promoter 2240..2245 /label=-35 promoter 2262..2267 /label=-10 misc_feature 2273..2273 /label=start misc_feature 2221..2275 /label=BBa_R0062 promoter 2221..2275 /label=BBa_R0062 misc_feature 2288..2291 /label=conserved RBS 2284..2295 /label=BBa_B0034 misc_feature 2881..2912 /label=LVA CDS 2302..2880 /label=luxI misc_feature 2302..2919 /label=BBa_C0061 misc_feature 2920..2944 /label=Help:Barcodes CDS 2302..2919 /label=BBa_C0061 stem_loop 2964..3007 /label=stem_loop misc_feature 2953..3032 /label=BBa_B0010 terminator 2953..3032 /label=BBa_B0010 stem_loop 3048..3067 /label=T7 TE misc_feature 3074..3074 /label=stop polyA_site 3068..3081 /label=polya misc_feature 3041..3081 /label=BBa_B0012 terminator 3041..3081 /label=BBa_B0012 terminator 2953..3081 /label=BBa_B0015 misc_feature 3090..3109 /label=LuxR/HSL promoter 3109..3114 /label=-35 promoter 3131..3136 /label=-10 misc_feature 3142..3142 /label=start misc_feature 3090..3144 /label=BBa_R0062 promoter 3090..3144 /label=BBa_R0062 misc_feature 3151..3153 /label=start misc_feature 3934..3939 /label=2 misc_feature 3901..3933 /label=LVA CDS 3151..3900 /label=aiiA misc_feature 3151..3939 /label=BBa_C0060 misc_feature 3940..3964 /label=Help:Barcodes CDS 3151..3939 /label=BBa_C0060 stem_loop 3984..4027 /label=stem_loop misc_feature 3973..4052 /label=BBa_B0010 terminator 3973..4052 /label=BBa_B0010 stem_loop 4068..4087 /label=T7 TE misc_feature 4094..4094 /label=stop polyA_site 4088..4101 /label=polya misc_feature 4061..4101 /label=BBa_B0012 terminator 4061..4101 /label=BBa_B0012 terminator 3973..4101 /label=BBa_B0015 misc_feature 4110..4129 /label=LuxR/HSL promoter 4129..4134 /label=-35 promoter 4151..4156 /label=-10 misc_feature 4162..4162 /label=start misc_feature 4110..4164 /label=BBa_R0062 promoter 4110..4164 /label=BBa_R0062 stem_loop 4184..4227 /label=stem_loop misc_feature 4173..4252 /label=BBa_B0010 terminator 4173..4252 /label=BBa_B0010 stem_loop 4268..4287 /label=T7 TE misc_feature 4294..4294 /label=stop polyA_site 4288..4301 /label=polya misc_feature 4261..4301 /label=BBa_B0012 terminator 4261..4301 /label=BBa_B0012 terminator 4173..4301 /label=BBa_B0015 ORIGIN 1 ggccgctgcg atcccgcgaa gaaccaaaaa agctcgacag agggcgcggt cattttaggt 61 cgggcggatc ggcgccgccg gctcggctgg tgtgccgcac agcaccgcct acgtgagctg 121 ccagttgatg aacttccccc gttgccagct agggcgcaag cgggctgtat aagatcactg 181 cccatcacat tgatcggctc ggattttttc tcaatccgta aacaggtcaa acatcagttg 241 ccgcaaccaa atattggcta ggtccttgtg gtacttcgca tgccagaaca tgttgatggc 301 tatttcaggc aagacgactg ggtgcggcaa ggcgcttagg ccgaagggct ccacgcagca 361 gtcggctaaa cgtatcggca cagtggcgag cagatcggtg cgctggagga tgtggccaac 421 ggcggcgaag tgcggcactt ccagacggat gtcgcgccgg atgccgaccc gtgtcatgta 481 cgtgtccacc tcgccgtggc cggtgccagc ggcgatgaca cgcacgtggc cgtaggaaca 541 gaagcgctcc agagtcaggg gttcgcgggt gactggatgg tccttgcgac ataggcacac 601 gtagtgattc tggagcagcc ggcgctgaaa gaagccagtt tgcagattgg gaagcaggcc 661 cacggccaag tccacggttc cgttctgcaa ggcctgcatc aggctcatcg aactgtcgcg 721 caccgtactg atcacgcaat tgggggcctg gtgagccagc acatccatca gccgcggcat 781 gaagtagatc tcgccaatgt cggtcatggc cagggtgaag gtacgctcgc tggtcagcgg 841 atcgaagctt tcatggtgct gtagggcgtt gcgcagtgcg tgcatggccg aagtgacggg 901 ctcggccaga tgcgcggcat agggtgtggg ttccattccc tgatgtgtgc gcacgaagag 961 tgggtcctgt agcgaggtgc gcaggcgttt cagcgcattg ctcacggcag gctgggtcag 1021 gcccaggttc tccgcagtga tagagacgcg tctgtcgacc agcaactggt tgaacaccac 1081 cagcaggttt aaatccaggt cacgcagttc catggggcct cgcttgggtt attgctggtg 1141 cccggccggg cgcaatattc atgttgatga tttattatat atcgagtggt gtatttatca 1201 atattgtttg ctccgttatc gttattaaca agtcatcaat aaagccatca cgagtaccat 1261 agaggatcta ctagagaaag aggagaaata ctagatgaaa aacataaatg ccgacgacac 1321 atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta atcaatgctt 1381 atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca tttatcctca 1441 ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat ggaggcaata 1501 ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta actccaatca 1561 ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat ctccaaatgt 1621 aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta ttcatacggc 1681 taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact atatagatag 1741 tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag ttgataatta 1801 tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga 1861 atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa tattaggttg 1921 cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca atacaacaaa 1981 ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc catactttaa 2041 aaattaataa cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa 2101 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 2161 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 2221 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 2281 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 2341 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 2401 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 2461 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 2521 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 2581 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 2641 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 2701 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 2761 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 2821 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 2881 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 2941 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 3001 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 3061 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 3121 aatggtttgt tatagtcgaa taaatactag atgacagtaa agaagcttta tttcgtccca 3181 gcaggtcgtt gtatgttgga tcattcgtct gttaatagta cattaacacc aggagaatta 3241 ttagacttac cggtttggtg ttatcttttg gagactgaag aaggacctat tttagtagat 3301 acaggtatgc cagaaagtgc agttaataat gaaggtcttt ttaacggtac atttgtcgaa 3361 gggcaggttt taccgaaaat gactgaagaa gatagaatcg tgaatatttt aaaacgggtt 3421 ggttatgagc cggaagacct tctttatatt attagttctc acttgcattt tgatcatgca 3481 ggaggaaatg gcgcttttat aaatacacca atcattgtac agcgtgctga atatgaggcg 3541 gcgcagcata gcgaagaata tttgaaagaa tgtatattgc cgaatttaaa ctacaaaatc 3601 attgaaggtg attatgaagt cgtaccagga gttcaattat tgcatacacc aggccatact 3661 ccagggcatc aatcgctatt aattgagaca gaaaaatccg gtcctgtatt attaacgatt 3721 gatgcatcgt atacgaaaga gaattttgaa aatgaagtgc catttgcggg atttgattca 3781 gaattagctt tatcttcaat taaacgttta aaagaagtgg tgatgaaaga gaagccgatt 3841 gttttctttg gacatgatat agagcaggaa aggggatgta aagtgttccc tgaatatata 3901 gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg ctagtgtaga 3961 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 4021 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 4081 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 4141 aatggtttgt tatagtcgaa taaatactag agccaggcat caaataaaac gaaaggctca 4201 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 4261 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //