LOCUS       BBa_K177000              976 bp    DNA     linear       22-Dec-2024
DEFINITION  Bistable_I part L
ACCESSION   BBa_K177000
VERSION     BBa_K177000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     RBS             46..58
                     /label=RBS-3\Weak
     RBS             52..55
                     /label=RBS
     misc_feature    46..58
                     /label=BBa_B0032
     RBS             46..58
                     /label=BBa_B0032
     CDS             68..775
                     /label=cI lambda
     misc_feature    776..808
                     /label=LVA
     misc_feature    815..839
                     /label=Help:Barcodes
     CDS             65..839
                     /label=BBa_C0051
     stem_loop       859..902
                     /label=stem_loop
     misc_feature    848..927
                     /label=BBa_B0010
     terminator      848..927
                     /label=BBa_B0010
     stem_loop       943..962
                     /label=T7 TE
     misc_feature    969..969
                     /label=stop
     polyA_site      963..976
                     /label=polya
     misc_feature    936..976
                     /label=BBa_B0012
     terminator      936..976
                     /label=BBa_B0012
     terminator      848..976
                     /label=BBa_B0015
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagtcaca caggaaagta
       61 ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct
      121 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga
      181 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa
      241 tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc
      301 ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag
      361 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct
      421 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga
      481 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc
      541 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga
      601 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag
      661 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag
      721 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc
      781 aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct
      841 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      901 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      961 cctttctgcg tttata
//