LOCUS       BBa_K177001             1105 bp    DNA     linear       23-Dec-2024
DEFINITION  Bistable_2_L
ACCESSION   BBa_K177001
VERSION     BBa_K177001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..957
                     /label=araC
     misc_feature    958..990
                     /label=LVA
     misc_feature    997..1021
                     /label=Help:Barcodes
     CDS             82..1021
                     /label=BBa_C0080
     stem_loop       1039..1054
                     /label=stem loop
     polyA_site      1055..1063
                     /label=Poly A tail
     polyA_site      1030..1038
                     /label=PolyA tail
     misc_feature    1030..1063
                     /label=B1001
     terminator      1030..1063
                     /label=BBa_B1001
     stem_loop       1081..1096
                     /label=stem loop
     polyA_site      1097..1105
                     /label=Poly A tail
     polyA_site      1072..1080
                     /label=PolyA tail
     misc_feature    1072..1105
                     /label=B1001
     terminator      1072..1105
                     /label=BBa_B1001
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatggctgaa gcgcaaaatg atcccctgct gccgggatac
      121 tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaatgg ttatctcgat
      181 ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt
      241 cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga tattttgctg
      301 ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac
      361 cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata
      421 tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg
      481 tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata
      541 aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca
      601 ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc
      661 aattttgata tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg tctgtcacat
      721 cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca acgcatcagc
      781 caggcgaagc tgcttttgag cactacccgg atgcctatcg ccaccgtcgg tcgcaatgtt
      841 ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg ggccagcccg
      901 agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcagct
      961 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
     1021 ctactagaga aaaaaaaacc ccgcttcggc ggggtttttt ttttactaga gaaaaaaaaa
     1081 ccccgcttcg gcggggtttt ttttt
//