##gff-version 3
##sequence-region BBa_K177001 1 1105
BBa_K177001	.	terminator	1030	1063	.	+	0	ID=BBa_B1001;Name=BBa_B1001
BBa_K177001	.	stem_loop	1039	1054	.	+	0	ID=annotation1898379;Name=stem loop;Parent=BBa_B1001
BBa_K177001	.	engineered_region	1030	1063	.	+	0	ID=annotation1898382;Name=B1001;Parent=BBa_B1001
BBa_K177001	.	polyA_site	1055	1063	.	+	0	ID=annotation1898380;Name=Poly A tail;Parent=BBa_B1001
BBa_K177001	.	polyA_site	1030	1038	.	+	0	ID=annotation1898381;Name=PolyA tail;Parent=BBa_B1001
BBa_K177001	.	CDS	82	1021	.	+	0	ID=BBa_C0080;Name=BBa_C0080
BBa_K177001	.	engineered_tag	997	1021	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_K177001	.	tag	958	990	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_K177001	.	CDS	82	957	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_K177001	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K177001	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K177001	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K177001	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K177001	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K177001	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K177001	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K177001	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177001	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177001	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177001	.	terminator	1072	1105	.	+	0	ID=BBa_B1001;Name=BBa_B1001
BBa_K177001	.	stem_loop	1081	1096	.	+	0	ID=annotation1898379;Name=stem loop;Parent=BBa_B1001
BBa_K177001	.	engineered_region	1072	1105	.	+	0	ID=annotation1898382;Name=B1001;Parent=BBa_B1001
BBa_K177001	.	polyA_site	1097	1105	.	+	0	ID=annotation1898380;Name=Poly A tail;Parent=BBa_B1001
BBa_K177001	.	polyA_site	1072	1080	.	+	0	ID=annotation1898381;Name=PolyA tail;Parent=BBa_B1001
>BBa_K177001
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgat
tgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgc
ggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagaga
ttcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggca
tgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagc
gacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagc
aattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtca
gtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgt
ctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagc
tgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcg
agtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtc
aagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agaaaaaaaaaccccgcttcggcggggttttttttttactagagaaaaaaaaaccccgcttcggcggggttttttttt