LOCUS       BBa_K177003             1809 bp    DNA     linear       22-Dec-2024
DEFINITION  Bistable_2_R
ACCESSION   BBa_K177003
VERSION     BBa_K177003.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     RBS             158..170
                     /label=RBS-3\Weak
     RBS             164..167
                     /label=RBS
     misc_feature    158..170
                     /label=BBa_B0032
     RBS             158..170
                     /label=BBa_B0032
     CDS             180..796
                     /label=tetR
     misc_feature    797..830
                     /label=SsrA
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             177..861
                     /label=BBa_C0040
     misc_feature    870..886
                     /label=OR1
     promoter        870..886
                     /label=BBa_K177002
     RBS             895..907
                     /label=RBS-3\Weak
     RBS             901..904
                     /label=RBS
     misc_feature    895..907
                     /label=BBa_B0032
     RBS             895..907
                     /label=BBa_B0032
     misc_feature    914..916
                     /label=start
     misc_feature    1667..1669
                     /label=stop
     misc_feature    1670..1672
                     /label=stop
     misc_feature    1630..1666
                     /label=LVA tag
     CDS             917..1629
                     /label=cds
     misc_feature    914..1672
                     /label=BBa_K145015
     stem_loop       1692..1735
                     /label=stem_loop
     misc_feature    1681..1760
                     /label=BBa_B0010
     terminator      1681..1760
                     /label=BBa_B0010
     stem_loop       1776..1795
                     /label=T7 TE
     misc_feature    1802..1802
                     /label=stop
     polyA_site      1796..1809
                     /label=polya
     misc_feature    1769..1809
                     /label=BBa_B0012
     terminator      1769..1809
                     /label=BBa_B0012
     terminator      1681..1809
                     /label=BBa_B0015
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagagtca cacaggaaag tactagatgt
      181 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
      241 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
      301 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
      361 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
      421 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
      481 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
      541 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
      601 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
      661 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
      721 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
      781 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact
      841 gatagtgcta gtgtagatca ctactagagt acctctggcg gtgatatact agagtcacac
      901 aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
      961 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1021 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1081 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1141 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1201 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1261 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1321 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1381 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1441 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1501 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1561 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1621 atacaaaagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag
     1681 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1741 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1801 gcgtttata
//