##gff-version 3
##sequence-region BBa_K177003 1 1809
BBa_K177003	.	CDS	177	861	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K177003	.	engineered_tag	837	861	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K177003	.	CDS	180	796	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K177003	.	tag	797	830	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K177003	.	promoter	1	149	.	+	0	ID=BBa_R0080;Name=BBa_R0080
BBa_K177003	.	promoter	113	118	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_K177003	.	non_covalent_binding_site	43	72	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_K177003	.	operator	6	44	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_K177003	.	non_covalent_binding_site	4	29	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_K177003	.	non_covalent_binding_site	73	101	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_K177003	.	promoter	136	141	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_K177003	.	ribosome_entry_site	895	907	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K177003	.	ribosome_entry_site	895	907	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177003	.	engineered_region	895	907	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177003	.	ribosome_entry_site	901	904	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177003	.	promoter	870	886	.	+	0	ID=BBa_K177002;Name=BBa_K177002
BBa_K177003	.	operator	870	886	.	+	0	ID=annotation2000761;Name=OR1;Parent=BBa_K177002
BBa_K177003	.	terminator	1681	1809	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K177003	.	terminator	1769	1809	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K177003	.	polyA_site	1796	1809	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177003	.	stop_codon	1802	1802	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177003	.	stem_loop	1776	1795	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177003	.	engineered_region	1769	1809	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177003	.	terminator	1681	1760	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K177003	.	stem_loop	1692	1735	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K177003	.	engineered_region	1681	1760	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177003	.	ribosome_entry_site	158	170	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K177003	.	ribosome_entry_site	158	170	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177003	.	engineered_region	158	170	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177003	.	ribosome_entry_site	164	167	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177003	.	engineered_region	914	1672	.	+	0	ID=BBa_K145015;Name=BBa_K145015
BBa_K177003	.	CDS	917	1629	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K177003	.	stop_codon	1667	1669	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K177003	.	tag	1630	1666	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K177003	.	stop_codon	1670	1672	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K177003	.	start_codon	914	916	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
>BBa_K177003
gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccata
gcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagt
cacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
tacctctggcggtgatatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgt
cccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaaca
tacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcg
gttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaagg
ttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgat
acccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaataca
actataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaa
cattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacca
gacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
ttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgcttt
agtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata