LOCUS BBa_K177004 8764 bp DNA linear 05-Nov-2024 DEFINITION bistable_1_R ACCESSION BBa_K177004 VERSION BBa_K177004.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 RBS 58..70 /label=RBS-3\Weak RBS 64..67 /label=RBS misc_feature 58..70 /label=BBa_B0032 RBS 58..70 /label=BBa_B0032 misc_feature 1166..1204 /label=LVA CDS 77..1204 /label=lacI-LVA misc_feature 77..1204 /label=BBa_C0012 misc_feature 1205..1229 /label=Help:Barcodes CDS 77..1204 /label=BBa_C0012 RBS 1238..1250 /label=RBS-3\Weak RBS 1244..1247 /label=RBS misc_feature 1238..1250 /label=BBa_B0032 RBS 1238..1250 /label=BBa_B0032 CDS 1257..4220 /label=BBa_I10001 CDS 4227..5755 /label=BBa_J61044 CDS 5764..6438 /label=PhoP misc_feature 6438..7901 /label=PhoQ CDS 5764..7901 /label=BBa_K177007 misc_feature 7911..7946 /label=gtc ...gtt -> gta ... gtt codon shuffle misc_feature 7908..8627 /label=BBa_Z0301 stem_loop 8647..8690 /label=stem_loop misc_feature 8636..8715 /label=BBa_B0010 terminator 8636..8715 /label=BBa_B0010 stem_loop 8731..8750 /label=T7 TE misc_feature 8757..8757 /label=stop polyA_site 8751..8764 /label=polya misc_feature 8724..8764 /label=BBa_B0012 terminator 8724..8764 /label=BBa_B0012 terminator 8636..8764 /label=BBa_B0015 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca 61 cacaggaaag tactagatgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta 121 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc 181 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt 241 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc 301 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc 361 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca 421 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga 481 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga 541 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga 601 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt 661 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc 721 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat 781 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg 841 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg 901 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga 961 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc 1021 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc 1081 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 1141 ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt tagtagctta 1201 ataactctga tagtgctagt gtagatctct actagagtca cacaggaaag tactagatga 1261 tggttttcca gccgatctct gagtttctgc tgatccgtaa cgctggtatg tctatgtact 1321 tcaacaaaat catctctttc aacatcatct ctcgtatcgt tatctgcatc ttcctgatct 1381 gcggtatgtt catggctggt gcttctgaaa aatacgacgc taacgctccg cagcaggttc 1441 agccgtactc tgtttcttct tctgctttcg aaaacctgca cccgaacaac gaaatggaat 1501 cttctatcaa cccgttctct gcttctgaca ccgaacgtaa cgctgctatc atcgaccgtg 1561 ctaacaaaga acaggaaacc gaagctgtta acaaaatgat ctctaccggt gctcgtctgg 1621 ctgcttctgg tcgtgcttct gacgttgctc actctatggt tggtgacgct gttaaccagg 1681 aaatcaaaca gtggctgaac cgtttcggta ccgctcaggt taacctgaac ttcgacaaaa 1741 acttctctct gaaagaatct tctctggact ggctggctcc gtggtacgac tctgcttctt 1801 tcctgttctt ctctcagctg ggtatccgta acaaagactc tcgtaacacc ctgaacctgg 1861 gtgttggtat ccgtaccctg gaaaacggtt ggctgtacgg tctgaacacc ttctacgaca 1921 acgacctgac cggtcacaac caccgtatcg gtctgggtgc tgaagcttgg accgactacc 1981 tccaactggc tgctaacggt tacttccgtc tgaacggttg gcactcttct cgtgacttct 2041 ctgactacaa agaacgtccg gctaccggtg gtgacctgcg tgctaacgct tacctgccgg 2101 ctctgccgca gctgggtggt aaactgatgt acgaacagta caccggtgaa cgtgttgctc 2161 tgttcggtaa agacaacctc caacgtaacc cgtacgctgt taccgctggt atcaactaca 2221 ccccggttcc gctgctgacc gttggtgttg accagcgtat gggtaaatct tctaaacacg 2281 aaacccagtg gaacctccaa atgaactacc gtctgggtga atctttccag tctcagctgt 2341 ctccgtctgc tgttgctggt acccgtctgc tggctgaatc tcgttacaac ctggttgacc 2401 gtaacaacaa catcgttctg gaataccaga aacagcaggt tgttaaactg accctgtctc 2461 cggctaccat ctctggtctg ccgggtcagg tttaccaggt taacgctcag gttcagggtg 2521 cttctgctgt tcgtgaaatc gtttggtctg acgctgaact gatcgctgct ggtggtaccc 2581 tgaccccgct gtctaccacc cagttcaacc tggttctgcc gccgtacaaa cgtaccgctc 2641 aggtttctcg tgttaccgac gacctgaccg ctaacttcta ctctctgtct gctctggctg 2701 ttgaccacca gggtaaccgt tctaactctt tcaccctgtc tgttaccgtt cagcagccgc 2761 agctgaccct gaccgctgct gttatcggtg acggtgctcc ggctaacggt aaaaccgcta 2821 tcaccgttga gtttaccgtt gctgacttcg aaggtaaacc gctggctggt caggaagttg 2881 ttatcaccac caacaacggt gctctgccga acaaaatcac cgaaaaaacc gacgctaacg 2941 gtgttgctcg tatcgctctg accaacacca ccgacggtgt taccgttgtt accgctgaag 3001 ttgaaggtca gcgtcagtct gttgacaccc acttcgttaa aggtaccatc gctgctgaca 3061 aatctaccct ggctgctgtt ccgacctcta tcatcgctga cggtctgatg gcttctacca 3121 tcaccctgga actgaaagac acctacggtg acccgcaggc tggtgctaac gttgctttcg 3181 acaccaccct gggtaacatg ggtgttatca ccgaccacaa cgacggtacc tactctgctc 3241 cgctgacctc taccaccctg ggtgttgcta ccgttaccgt taaagttgac ggtgctgctt 3301 tctctgttcc gtctgttacc gttaacttca ccgctgaccc gatcccggac gctggtcgtt 3361 cttctttcac cgtttctacc ccggacatcc tggctgacgg taccatgtct tctaccctgt 3421 ctttcgttcc ggttgacaaa aacggtcact tcatctctgg tatgcagggt ctgtctttca 3481 cccagaacgg tgttccggtt tctatctctc cgatcaccga acagccggac tcttacaccg 3541 ctaccgttgt tggtaactct gttggtgacg ttaccatcac cccgcaggtt gacaccctga 3601 tcctgtctac cctccaaaaa aaaatctctc tgttcccggt tccgaccctg accggtatcc 3661 tggttaacgg tcagaacttc gctaccgaca aaggtttccc gaaaaccatc ttcaaaaacg 3721 ctaccttcca gctccaaatg gacaacgacg ttgctaacaa cacccagtac gaatggtctt 3781 cttctttcac cccgaacgtt tctgttaacg accagggtca ggttaccatc acctaccaga 3841 cctactctga agttgctgtt accgctaaat ctaaaaaatt cccgtcttac tctgtttctt 3901 accgtttcta cccgaaccgt tggatctacg acggtggtcg ttctctggtt tcttctctgg 3961 aagcttctcg tcagtgccag ggttctgaca tgtctgctgt tctggaatct tctcgtgcta 4021 ccaacggtac ccgtgctccg gacggtaccc tgtggggtga atggggttct ctgaccgctt 4081 actcttctga ctggcagtct ggtgaatact gggttaaaaa aacctctacc gacttcgaaa 4141 ccatgaacat ggacaccggt gctctccaac cgggtccggc ttacctggct ttcccgctgt 4201 gcgctctgtc tatctaataa tactagatga aggatgcatc tgcattcaat aaagaaaatt 4261 caatttcatc catggcacca ccagcatctc cgcctgcaag tcctaagacg ccaatcgaaa 4321 agaaacacgc ggatgaaatc gataagtata tacaaggatt ggattacaat aaaaacaatg 4381 tattagtata ccacggagat gcagtgacaa atgtgccgcc aagaaaaggt tacaaagatg 4441 gaaatgaata tattgttgtg gagaaaaaga agaaatccat caatcaaaat aatgcagaca 4501 ttcaagttgt gaatgcaatt tcgagcctaa cctatccagg tgctctcgta aaagcgaact 4561 cggaattagt agaaaatcaa ccagatgttc tccctgtaaa acgtgattca ttaacactca 4621 gcattgattt gccaggtatg actaatcaag acaataaaat agttgtaaaa aatgccacta 4681 aatcaaacgt taacaacgca gtaaatacat tagtggaaag atggaatgaa aaatatgctc 4741 aagcttatcc aaatgtaagt gcaaaaattg attatgatga cgaaatggct tacagtgaat 4801 cacaattaat tgcgaaattt ggtacagcat ttaaagctgt aaataatagc ttgaatgtaa 4861 acttcggcgc aatcagtgaa gggaaaatgc aagaagaagt cattagtttt aaacaaattt 4921 actataacgt gaatgttaat gaacctacaa gaccttccag atttttcggc aaagctgtta 4981 ctaaagagca gttgcaagcg cttggagtga atgcagaaaa tcctcctgca tatatctcaa 5041 gtgtggcgta tggccgtcaa gtttatttga aattatcaac taattcccat agtactaaag 5101 taaaagctgc ttttgatgct gccgtaagcg gaaaatctgt ctcaggtgat gtagaactaa 5161 caaatatcat caaaaattct tccttcaaag ccgtaattta cggaggttcc gcaaaagatg 5221 aagttcaaat catcgacggc aacctcggag acttacgcga tattttgaaa aaaggcgcta 5281 cttttaatcg agaaacacca ggagttccca ttgcttatac aacaaacttc ctaaaagaca 5341 atgaattagc tgttattaaa aacaactcag aatatattga aacaacttca aaagcttata 5401 cagatggaaa aattaacatc gatcactctg gaggatacgt tgctcaattc aacatttctt 5461 gggatgaagt aaattatgat cctgaaggta acgaaattgt tcaacataaa aactggagcg 5521 aaaacaataa aagcaagcta gctcatttca catcgtccat ctatttgcct ggtaacgcga 5581 gaaatattaa tgtttacgct aaagaatgca ctggtttagc ttgggaatgg tggagaacgg 5641 taattgatga ccggaactta ccacttgtga aaaatagaaa tatctccatc tggggcacca 5701 cgctttatcc gaaatatagt aataaagtag ataatccaat cgaataataa gaatttacta 5761 gagctccagg attcaggtca ccaggtcgat gccgcagaag atgccaggga agctgattac 5821 taccttaatg aacaccttcc ggatatcgct attgtcgatt taggtctgcc ggatgaagac 5881 ggcctttcct taatacgccg ctggcgcagc agtgatgttt cactgccggt tctggtgtta 5941 accgcgcgcg aaggctggca ggataaagtc gaggttctca gctccggggc cgatgactac 6001 gtgacgaagc cattccacat cgaagaggta atggcgcgta tgcaggcgtt aatgcgccgt 6061 aatagcggtc tggcctccca ggtgatcaac atcccgccgt tccaggtgga tctctcacgc 6121 cgggaattat ccgtcaatga agaggtcatc aaactcacgg cgttcgaata caccattatg 6181 gaaacgctta tccgtaacaa cggtaaagtg gtcagcaaag attcgctgat gcttcagctg 6241 tatccggatg cggaactgcg ggaaagtcat accattgatg ttctcatggg gcgtctgcgg 6301 aaaaaaatac aggcccagta tccgcacgat gtcattacca ccgtacgcgg acaaggatat 6361 ctttttgaat tgcgctaatg aataaatttg ctcgccattt tctgccgctg tcgctgcggg 6421 ttcgtttttt gctggcgaca gccggcgtcg tgctggtgct ttctttggca tatggcatag 6481 tggcgctggt cggctatagc gtaagttttg ataaaaccac ctttcgtttg ctgcgcggcg 6541 aaagcaacct gttttatacc ctcgccaaat gggaaaataa taaaatcagc gttgagctgc 6601 ctgaaaatct ggacatgcaa agcccgacca tgacgctgat ttacgatgaa acgggcaaat 6661 tattatggac gcagcgcaac attccctggc tgattaaaag cattcaaccg gaatggttaa 6721 aaacgaacgg cttccatgaa attgaaacca acgtagacgc caccagcacg ctgttgagcg 6781 aagaccattc cgcgcaggaa aaactcaaag aagtacgtga agatgacgat gatgccgaga 6841 tgacccactc ggtagcggta aatatttatc ctgccacggc gcggatgccg cagttaacca 6901 tcgtggtggt cgataccatt ccgatagaac taaaacgctc ctatatggtg tggagctggt 6961 tcgtatacgt gctggccgcc aatttactgt tagtcattcc tttactgtgg atcgccgcct 7021 ggtggagctt acgccctatc gaggcgctgg cgcgggaagt ccgcgagctt gaagatcatc 7081 accgcgaaat gctcaatccg gagacgacgc gtgagctgac cagccttgtg cgcaacctta 7141 atcaactgct caaaagcgag cgtgaacgtt ataacaaata ccgcacgacc ctgaccgacc 7201 tgacgcacag tttaaaaacg ccgctcgcgg ttttgcagag tacgttacgc tctttacgca 7261 acgaaaagat gagcgtcagc aaagctgaac cggtgatgct ggaacagatc agccggattt 7321 cccagcagat cggctattat ctgcatcgcg ccagtatgcg cggtagcggc gtgttgttaa 7381 gccgcgaact gcatcccgtc gcgccgttgt tagataacct gatttctgcg ctaaataaag 7441 tttatcagcg taaaggggtg aatatcagta tggatatttc accagaaatc agttttgtcg 7501 gcgagcaaaa cgactttgtc gaagtgatgg gcaacgtact ggacaacgct tgtaaatatt 7561 gtctggagtt tgtcgagatt tcggctcgcc agaccgacga tcatttgcat attttcgtcg 7621 aagatgacgg cccaggcatt ccccacagca aacgttccct ggtgtttgat cgcggtcagc 7681 gcgccgatac cctacgacca ggacaaggcg tggggctggc tgtcgcgcgc gagattacgg 7741 aacaatacgc cgggcagatc attgccagcg acagtctgct cggtggcgcc cgtatggagg 7801 tcgtttttgg ccgacagcat cccacacaga aagaggaata aacatttctg tgcgttcttc 7861 cacgcatctc gttaagcatc cgttataatc ggtgtcagat atactagatg gtaagcaaag 7921 gagaggaatt atttacagga gttgttccga tcctggttga actggacggt gacgttaacg 7981 gtcacaaatt ctctgtttct ggtgaaggtg aaggtgacgc tacctacggt aaactgaccc 8041 tgaaactgat ctgcaccacc ggtaaactgc cggttccgtg gccgaccctc gtaactaccc 8101 tgggttacgg tctccaatgc ttcgctcgtt acccggacca catgaaacag cacgacttct 8161 tcaaatctgc tatgccggaa ggttacgttc aggaacgtac catcttcttc aaagacgacg 8221 gtaactacaa aacccgtgct gaagttaaat tcgaaggtga caccctggtt aaccgtatcg 8281 aactgaaagg tatcgacttc aaagaagacg gtaacatcct gggtcacaaa ctggaataca 8341 actacaactc tcacaacgtt tacatcaccg ctgacaaaca gaaaaacggt atcaaagcta 8401 acttcaaaat ccgtcacaac atcgaagacg gtggtgttca gctggctgac cactaccagc 8461 agaacacccc aattggtgat ggcccagttc tgctgccgga caaccactac ctgtcttacc 8521 agtctgctct gtctaaagac ccgaacgaaa aacgtgacca catggttctg ctggagtttg 8581 ttaccgctgc tggtatcacc ctgggtatgg acgaactgta caaataatac tagagccagg 8641 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 8701 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 8761 tata //