BBa_R0051
1
cI lam
promoter (lambda cI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
<a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
Released HQ 2013
The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor.
true
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
annotation2023
1
-35
range2023
1
15
20
annotation7067
1
BBa_R0051
range7067
1
1
49
annotation2024
1
OR1
range2024
1
25
41
annotation2025
1
OR2
range2025
1
1
17
annotation2022
1
-10
range2022
1
38
43
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K177007
1
BBa_K177007
PhoP/PhoQ locus from salmonella typhimurium
2009-02-18T12:00:00Z
2015-05-08T01:11:05Z
pH sensing locus from S. typh
false
false
_284_
0
2582
9
Not in stock
false
false
Michael Lower
annotation2000914
1
PhoQ
range2000914
1
675
2138
annotation2000913
1
PhoP
range2000913
1
1
675
BBa_C0012
1
lacI
lacI repressor from E. coli (+LVA)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.</P> <P>A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.</P>
false
false
_1_
0
24
7
In stock
false
References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br> <br> </P> <P> References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br> <br> </P> <P>Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation.<P> Incompatible with systems containing LacI, lactose, or IPTG.
true
Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
annotation1723
1
lacI-LVA
range1723
1
1
1128
annotation2213988
1
Help:Barcodes
range2213988
1
1129
1153
annotation7031
1
BBa_C0012
range7031
1
1
1128
annotation1722
1
LVA
range1722
1
1090
1128
BBa_J61044
1
BBa_J61044
[LLO] Listeriolysin open reading frame
2007-02-20T12:00:00Z
2015-08-31T02:03:00Z
bob
bob
false
false
_95_
0
483
95
Not in stock
false
bob
false
John Anderson
BBa_K177004
1
BBa_K177004
bistable_1_R
2009-02-18T12:00:00Z
2015-05-08T01:11:05Z
right portion of bistable 1 switch
false
false
_284_
0
2582
9
Not in stock
false
false
Michael Lower
component2283191
1
BBa_B0015
component2283179
1
BBa_J61044
component2283182
1
BBa_K177007
component2283169
1
BBa_B0032
component2283164
1
BBa_R0051
component2283176
1
BBa_B0032
component2283184
1
BBa_Z0301
component2283178
1
BBa_I10001
component2283172
1
BBa_C0012
annotation2283184
1
BBa_Z0301
range2283184
1
7908
8627
annotation2283164
1
BBa_R0051
range2283164
1
1
49
annotation2283178
1
BBa_I10001
range2283178
1
1257
4220
annotation2283172
1
BBa_C0012
range2283172
1
77
1204
annotation2283182
1
BBa_K177007
range2283182
1
5764
7901
annotation2283169
1
BBa_B0032
range2283169
1
58
70
annotation2283176
1
BBa_B0032
range2283176
1
1238
1250
annotation2283179
1
BBa_J61044
range2283179
1
4227
5755
annotation2283191
1
BBa_B0015
range2283191
1
8636
8764
BBa_I10001
1
BBa_I10001
Invasin
2006-04-03T11:00:00Z
2015-08-31T04:07:29Z
Invasin is a bacterial membrane protein (from Y.pseudotuberculosis) that binds tightly to beta-1-integrins found on the surface of many mamallian cells; interaction between invasin and beta-1-integrins induces the physical uptake of bacteria by mamallian cells via a Rac-1 mediated response (Wong & Isberg, 2005; PMID: 15694850)
false
false
_89_45_47_11_12_21_8_48_46_1_10_43_
0
52
89
Not in stock
false
false
Chris Anderson (Voigt Lab)
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
BBa_Z0301
1
BBa_Z0301
Venus YFP - E. coli codon optimized
2006-06-20T11:00:00Z
2015-05-08T01:14:55Z
Venus Sequence obtained from Prof. Miyawaki. Codon optimized from Gene Design by Boeke Lab.
Brighter Variant than standard EYFP.
false
false
_11_
0
64
10
It's complicated
false
None really
false
Sriram Kosuri
annotation1881736
1
gtc ...gtt -> gta ... gtt codon shuffle
range1881736
1
4
39
BBa_K177004_sequence
1
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacaggaaagtactagatgatggttttccagccgatctctgagtttctgctgatccgtaacgctggtatgtctatgtacttcaacaaaatcatctctttcaacatcatctctcgtatcgttatctgcatcttcctgatctgcggtatgttcatggctggtgcttctgaaaaatacgacgctaacgctccgcagcaggttcagccgtactctgtttcttcttctgctttcgaaaacctgcacccgaacaacgaaatggaatcttctatcaacccgttctctgcttctgacaccgaacgtaacgctgctatcatcgaccgtgctaacaaagaacaggaaaccgaagctgttaacaaaatgatctctaccggtgctcgtctggctgcttctggtcgtgcttctgacgttgctcactctatggttggtgacgctgttaaccaggaaatcaaacagtggctgaaccgtttcggtaccgctcaggttaacctgaacttcgacaaaaacttctctctgaaagaatcttctctggactggctggctccgtggtacgactctgcttctttcctgttcttctctcagctgggtatccgtaacaaagactctcgtaacaccctgaacctgggtgttggtatccgtaccctggaaaacggttggctgtacggtctgaacaccttctacgacaacgacctgaccggtcacaaccaccgtatcggtctgggtgctgaagcttggaccgactacctccaactggctgctaacggttacttccgtctgaacggttggcactcttctcgtgacttctctgactacaaagaacgtccggctaccggtggtgacctgcgtgctaacgcttacctgccggctctgccgcagctgggtggtaaactgatgtacgaacagtacaccggtgaacgtgttgctctgttcggtaaagacaacctccaacgtaacccgtacgctgttaccgctggtatcaactacaccccggttccgctgctgaccgttggtgttgaccagcgtatgggtaaatcttctaaacacgaaacccagtggaacctccaaatgaactaccgtctgggtgaatctttccagtctcagctgtctccgtctgctgttgctggtacccgtctgctggctgaatctcgttacaacctggttgaccgtaacaacaacatcgttctggaataccagaaacagcaggttgttaaactgaccctgtctccggctaccatctctggtctgccgggtcaggtttaccaggttaacgctcaggttcagggtgcttctgctgttcgtgaaatcgtttggtctgacgctgaactgatcgctgctggtggtaccctgaccccgctgtctaccacccagttcaacctggttctgccgccgtacaaacgtaccgctcaggtttctcgtgttaccgacgacctgaccgctaacttctactctctgtctgctctggctgttgaccaccagggtaaccgttctaactctttcaccctgtctgttaccgttcagcagccgcagctgaccctgaccgctgctgttatcggtgacggtgctccggctaacggtaaaaccgctatcaccgttgagtttaccgttgctgacttcgaaggtaaaccgctggctggtcaggaagttgttatcaccaccaacaacggtgctctgccgaacaaaatcaccgaaaaaaccgacgctaacggtgttgctcgtatcgctctgaccaacaccaccgacggtgttaccgttgttaccgctgaagttgaaggtcagcgtcagtctgttgacacccacttcgttaaaggtaccatcgctgctgacaaatctaccctggctgctgttccgacctctatcatcgctgacggtctgatggcttctaccatcaccctggaactgaaagacacctacggtgacccgcaggctggtgctaacgttgctttcgacaccaccctgggtaacatgggtgttatcaccgaccacaacgacggtacctactctgctccgctgacctctaccaccctgggtgttgctaccgttaccgttaaagttgacggtgctgctttctctgttccgtctgttaccgttaacttcaccgctgacccgatcccggacgctggtcgttcttctttcaccgtttctaccccggacatcctggctgacggtaccatgtcttctaccctgtctttcgttccggttgacaaaaacggtcacttcatctctggtatgcagggtctgtctttcacccagaacggtgttccggtttctatctctccgatcaccgaacagccggactcttacaccgctaccgttgttggtaactctgttggtgacgttaccatcaccccgcaggttgacaccctgatcctgtctaccctccaaaaaaaaatctctctgttcccggttccgaccctgaccggtatcctggttaacggtcagaacttcgctaccgacaaaggtttcccgaaaaccatcttcaaaaacgctaccttccagctccaaatggacaacgacgttgctaacaacacccagtacgaatggtcttcttctttcaccccgaacgtttctgttaacgaccagggtcaggttaccatcacctaccagacctactctgaagttgctgttaccgctaaatctaaaaaattcccgtcttactctgtttcttaccgtttctacccgaaccgttggatctacgacggtggtcgttctctggtttcttctctggaagcttctcgtcagtgccagggttctgacatgtctgctgttctggaatcttctcgtgctaccaacggtacccgtgctccggacggtaccctgtggggtgaatggggttctctgaccgcttactcttctgactggcagtctggtgaatactgggttaaaaaaacctctaccgacttcgaaaccatgaacatggacaccggtgctctccaaccgggtccggcttacctggctttcccgctgtgcgctctgtctatctaataatactagatgaaggatgcatctgcattcaataaagaaaattcaatttcatccatggcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttacaaagatggaaatgaatatattgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagacattcaagttgtgaatgcaatttcgagcctaacctatccaggtgctctcgtaaaagcgaactcggaattagtagaaaatcaaccagatgttctccctgtaaaacgtgattcattaacactcagcattgatttgccaggtatgactaatcaagacaataaaatagttgtaaaaaatgccactaaatcaaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcgaaatttggtacagcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcgtatggccgtcaagtttatttgaaattatcaactaattcccatagtactaaagtaaaagctgcttttgatgctgccgtaagcggaaaatctgtctcaggtgatgtagaactaacaaatatcatcaaaaattcttccttcaaagccgtaatttacggaggttccgcaaaagatgaagttcaaatcatcgacggcaacctcggagacttacgcgatattttgaaaaaaggcgctacttttaatcgagaaacaccaggagttcccattgcttatacaacaaacttcctaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaattaacatcgatcactctggaggatacgttgctcaattcaacatttcttgggatgaagtaaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagcaagctagctcatttcacatcgtccatctatttgcctggtaacgcgagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacttaccacttgtgaaaaatagaaatatctccatctggggcaccacgctttatccgaaatatagtaataaagtagataatccaatcgaataataagaatttactagagctccaggattcaggtcaccaggtcgatgccgcagaagatgccagggaagctgattactaccttaatgaacaccttccggatatcgctattgtcgatttaggtctgccggatgaagacggcctttccttaatacgccgctggcgcagcagtgatgtttcactgccggttctggtgttaaccgcgcgcgaaggctggcaggataaagtcgaggttctcagctccggggccgatgactacgtgacgaagccattccacatcgaagaggtaatggcgcgtatgcaggcgttaatgcgccgtaatagcggtctggcctcccaggtgatcaacatcccgccgttccaggtggatctctcacgccgggaattatccgtcaatgaagaggtcatcaaactcacggcgttcgaatacaccattatggaaacgcttatccgtaacaacggtaaagtggtcagcaaagattcgctgatgcttcagctgtatccggatgcggaactgcgggaaagtcataccattgatgttctcatggggcgtctgcggaaaaaaatacaggcccagtatccgcacgatgtcattaccaccgtacgcggacaaggatatctttttgaattgcgctaatgaataaatttgctcgccattttctgccgctgtcgctgcgggttcgttttttgctggcgacagccggcgtcgtgctggtgctttctttggcatatggcatagtggcgctggtcggctatagcgtaagttttgataaaaccacctttcgtttgctgcgcggcgaaagcaacctgttttataccctcgccaaatgggaaaataataaaatcagcgttgagctgcctgaaaatctggacatgcaaagcccgaccatgacgctgatttacgatgaaacgggcaaattattatggacgcagcgcaacattccctggctgattaaaagcattcaaccggaatggttaaaaacgaacggcttccatgaaattgaaaccaacgtagacgccaccagcacgctgttgagcgaagaccattccgcgcaggaaaaactcaaagaagtacgtgaagatgacgatgatgccgagatgacccactcggtagcggtaaatatttatcctgccacggcgcggatgccgcagttaaccatcgtggtggtcgataccattccgatagaactaaaacgctcctatatggtgtggagctggttcgtatacgtgctggccgccaatttactgttagtcattcctttactgtggatcgccgcctggtggagcttacgccctatcgaggcgctggcgcgggaagtccgcgagcttgaagatcatcaccgcgaaatgctcaatccggagacgacgcgtgagctgaccagccttgtgcgcaaccttaatcaactgctcaaaagcgagcgtgaacgttataacaaataccgcacgaccctgaccgacctgacgcacagtttaaaaacgccgctcgcggttttgcagagtacgttacgctctttacgcaacgaaaagatgagcgtcagcaaagctgaaccggtgatgctggaacagatcagccggatttcccagcagatcggctattatctgcatcgcgccagtatgcgcggtagcggcgtgttgttaagccgcgaactgcatcccgtcgcgccgttgttagataacctgatttctgcgctaaataaagtttatcagcgtaaaggggtgaatatcagtatggatatttcaccagaaatcagttttgtcggcgagcaaaacgactttgtcgaagtgatgggcaacgtactggacaacgcttgtaaatattgtctggagtttgtcgagatttcggctcgccagaccgacgatcatttgcatattttcgtcgaagatgacggcccaggcattccccacagcaaacgttccctggtgtttgatcgcggtcagcgcgccgataccctacgaccaggacaaggcgtggggctggctgtcgcgcgcgagattacggaacaatacgccgggcagatcattgccagcgacagtctgctcggtggcgcccgtatggaggtcgtttttggccgacagcatcccacacagaaagaggaataaacatttctgtgcgttcttccacgcatctcgttaagcatccgttataatcggtgtcagatatactagatggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J61044_sequence
1
atgaaggatgcatctgcattcaataaagaaaattcaatttcatccatggcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttacaaagatggaaatgaatatattgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagacattcaagttgtgaatgcaatttcgagcctaacctatccaggtgctctcgtaaaagcgaactcggaattagtagaaaatcaaccagatgttctccctgtaaaacgtgattcattaacactcagcattgatttgccaggtatgactaatcaagacaataaaatagttgtaaaaaatgccactaaatcaaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcgaaatttggtacagcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcgtatggccgtcaagtttatttgaaattatcaactaattcccatagtactaaagtaaaagctgcttttgatgctgccgtaagcggaaaatctgtctcaggtgatgtagaactaacaaatatcatcaaaaattcttccttcaaagccgtaatttacggaggttccgcaaaagatgaagttcaaatcatcgacggcaacctcggagacttacgcgatattttgaaaaaaggcgctacttttaatcgagaaacaccaggagttcccattgcttatacaacaaacttcctaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaattaacatcgatcactctggaggatacgttgctcaattcaacatttcttgggatgaagtaaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagcaagctagctcatttcacatcgtccatctatttgcctggtaacgcgagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacttaccacttgtgaaaaatagaaatatctccatctggggcaccacgctttatccgaaatatagtaataaagtagataatccaatcgaataataagaatt
BBa_R0051_sequence
1
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
BBa_Z0301_sequence
1
atggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataa
BBa_B0032_sequence
1
tcacacaggaaag
BBa_I10001_sequence
1
atgatggttttccagccgatctctgagtttctgctgatccgtaacgctggtatgtctatgtacttcaacaaaatcatctctttcaacatcatctctcgtatcgttatctgcatcttcctgatctgcggtatgttcatggctggtgcttctgaaaaatacgacgctaacgctccgcagcaggttcagccgtactctgtttcttcttctgctttcgaaaacctgcacccgaacaacgaaatggaatcttctatcaacccgttctctgcttctgacaccgaacgtaacgctgctatcatcgaccgtgctaacaaagaacaggaaaccgaagctgttaacaaaatgatctctaccggtgctcgtctggctgcttctggtcgtgcttctgacgttgctcactctatggttggtgacgctgttaaccaggaaatcaaacagtggctgaaccgtttcggtaccgctcaggttaacctgaacttcgacaaaaacttctctctgaaagaatcttctctggactggctggctccgtggtacgactctgcttctttcctgttcttctctcagctgggtatccgtaacaaagactctcgtaacaccctgaacctgggtgttggtatccgtaccctggaaaacggttggctgtacggtctgaacaccttctacgacaacgacctgaccggtcacaaccaccgtatcggtctgggtgctgaagcttggaccgactacctccaactggctgctaacggttacttccgtctgaacggttggcactcttctcgtgacttctctgactacaaagaacgtccggctaccggtggtgacctgcgtgctaacgcttacctgccggctctgccgcagctgggtggtaaactgatgtacgaacagtacaccggtgaacgtgttgctctgttcggtaaagacaacctccaacgtaacccgtacgctgttaccgctggtatcaactacaccccggttccgctgctgaccgttggtgttgaccagcgtatgggtaaatcttctaaacacgaaacccagtggaacctccaaatgaactaccgtctgggtgaatctttccagtctcagctgtctccgtctgctgttgctggtacccgtctgctggctgaatctcgttacaacctggttgaccgtaacaacaacatcgttctggaataccagaaacagcaggttgttaaactgaccctgtctccggctaccatctctggtctgccgggtcaggtttaccaggttaacgctcaggttcagggtgcttctgctgttcgtgaaatcgtttggtctgacgctgaactgatcgctgctggtggtaccctgaccccgctgtctaccacccagttcaacctggttctgccgccgtacaaacgtaccgctcaggtttctcgtgttaccgacgacctgaccgctaacttctactctctgtctgctctggctgttgaccaccagggtaaccgttctaactctttcaccctgtctgttaccgttcagcagccgcagctgaccctgaccgctgctgttatcggtgacggtgctccggctaacggtaaaaccgctatcaccgttgagtttaccgttgctgacttcgaaggtaaaccgctggctggtcaggaagttgttatcaccaccaacaacggtgctctgccgaacaaaatcaccgaaaaaaccgacgctaacggtgttgctcgtatcgctctgaccaacaccaccgacggtgttaccgttgttaccgctgaagttgaaggtcagcgtcagtctgttgacacccacttcgttaaaggtaccatcgctgctgacaaatctaccctggctgctgttccgacctctatcatcgctgacggtctgatggcttctaccatcaccctggaactgaaagacacctacggtgacccgcaggctggtgctaacgttgctttcgacaccaccctgggtaacatgggtgttatcaccgaccacaacgacggtacctactctgctccgctgacctctaccaccctgggtgttgctaccgttaccgttaaagttgacggtgctgctttctctgttccgtctgttaccgttaacttcaccgctgacccgatcccggacgctggtcgttcttctttcaccgtttctaccccggacatcctggctgacggtaccatgtcttctaccctgtctttcgttccggttgacaaaaacggtcacttcatctctggtatgcagggtctgtctttcacccagaacggtgttccggtttctatctctccgatcaccgaacagccggactcttacaccgctaccgttgttggtaactctgttggtgacgttaccatcaccccgcaggttgacaccctgatcctgtctaccctccaaaaaaaaatctctctgttcccggttccgaccctgaccggtatcctggttaacggtcagaacttcgctaccgacaaaggtttcccgaaaaccatcttcaaaaacgctaccttccagctccaaatggacaacgacgttgctaacaacacccagtacgaatggtcttcttctttcaccccgaacgtttctgttaacgaccagggtcaggttaccatcacctaccagacctactctgaagttgctgttaccgctaaatctaaaaaattcccgtcttactctgtttcttaccgtttctacccgaaccgttggatctacgacggtggtcgttctctggtttcttctctggaagcttctcgtcagtgccagggttctgacatgtctgctgttctggaatcttctcgtgctaccaacggtacccgtgctccggacggtaccctgtggggtgaatggggttctctgaccgcttactcttctgactggcagtctggtgaatactgggttaaaaaaacctctaccgacttcgaaaccatgaacatggacaccggtgctctccaaccgggtccggcttacctggctttcccgctgtgcgctctgtctatctaataa
BBa_C0012_sequence
1
atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
BBa_K177007_sequence
1
ctccaggattcaggtcaccaggtcgatgccgcagaagatgccagggaagctgattactaccttaatgaacaccttccggatatcgctattgtcgatttaggtctgccggatgaagacggcctttccttaatacgccgctggcgcagcagtgatgtttcactgccggttctggtgttaaccgcgcgcgaaggctggcaggataaagtcgaggttctcagctccggggccgatgactacgtgacgaagccattccacatcgaagaggtaatggcgcgtatgcaggcgttaatgcgccgtaatagcggtctggcctcccaggtgatcaacatcccgccgttccaggtggatctctcacgccgggaattatccgtcaatgaagaggtcatcaaactcacggcgttcgaatacaccattatggaaacgcttatccgtaacaacggtaaagtggtcagcaaagattcgctgatgcttcagctgtatccggatgcggaactgcgggaaagtcataccattgatgttctcatggggcgtctgcggaaaaaaatacaggcccagtatccgcacgatgtcattaccaccgtacgcggacaaggatatctttttgaattgcgctaatgaataaatttgctcgccattttctgccgctgtcgctgcgggttcgttttttgctggcgacagccggcgtcgtgctggtgctttctttggcatatggcatagtggcgctggtcggctatagcgtaagttttgataaaaccacctttcgtttgctgcgcggcgaaagcaacctgttttataccctcgccaaatgggaaaataataaaatcagcgttgagctgcctgaaaatctggacatgcaaagcccgaccatgacgctgatttacgatgaaacgggcaaattattatggacgcagcgcaacattccctggctgattaaaagcattcaaccggaatggttaaaaacgaacggcttccatgaaattgaaaccaacgtagacgccaccagcacgctgttgagcgaagaccattccgcgcaggaaaaactcaaagaagtacgtgaagatgacgatgatgccgagatgacccactcggtagcggtaaatatttatcctgccacggcgcggatgccgcagttaaccatcgtggtggtcgataccattccgatagaactaaaacgctcctatatggtgtggagctggttcgtatacgtgctggccgccaatttactgttagtcattcctttactgtggatcgccgcctggtggagcttacgccctatcgaggcgctggcgcgggaagtccgcgagcttgaagatcatcaccgcgaaatgctcaatccggagacgacgcgtgagctgaccagccttgtgcgcaaccttaatcaactgctcaaaagcgagcgtgaacgttataacaaataccgcacgaccctgaccgacctgacgcacagtttaaaaacgccgctcgcggttttgcagagtacgttacgctctttacgcaacgaaaagatgagcgtcagcaaagctgaaccggtgatgctggaacagatcagccggatttcccagcagatcggctattatctgcatcgcgccagtatgcgcggtagcggcgtgttgttaagccgcgaactgcatcccgtcgcgccgttgttagataacctgatttctgcgctaaataaagtttatcagcgtaaaggggtgaatatcagtatggatatttcaccagaaatcagttttgtcggcgagcaaaacgactttgtcgaagtgatgggcaacgtactggacaacgcttgtaaatattgtctggagtttgtcgagatttcggctcgccagaccgacgatcatttgcatattttcgtcgaagatgacggcccaggcattccccacagcaaacgttccctggtgtttgatcgcggtcagcgcgccgataccctacgaccaggacaaggcgtggggctggctgtcgcgcgcgagattacggaacaatacgccgggcagatcattgccagcgacagtctgctcggtggcgcccgtatggaggtcgtttttggccgacagcatcccacacagaaagaggaataaacatttctgtgcgttcttccacgcatctcgttaagcatccgttataatcggtgtcagata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z