BBa_R0051 1 cI lam promoter (lambda cI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z <a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Released HQ 2013 The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P> false true _1_ 0 24 7 In stock false <P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor. true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation2023 1 -35 range2023 1 15 20 annotation7067 1 BBa_R0051 range7067 1 1 49 annotation2024 1 OR1 range2024 1 25 41 annotation2025 1 OR2 range2025 1 1 17 annotation2022 1 -10 range2022 1 38 43 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K177007 1 BBa_K177007 PhoP/PhoQ locus from salmonella typhimurium 2009-02-18T12:00:00Z 2015-05-08T01:11:05Z pH sensing locus from S. typh false false _284_ 0 2582 9 Not in stock false false Michael Lower annotation2000914 1 PhoQ range2000914 1 675 2138 annotation2000913 1 PhoP range2000913 1 1 675 BBa_C0012 1 lacI lacI repressor from E. coli (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.</P> <P>A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.</P> false false _1_ 0 24 7 In stock false References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br> <br> </P> <P> References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br> <br> </P> <P>Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation.<P> Incompatible with systems containing LacI, lactose, or IPTG. true Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton annotation1723 1 lacI-LVA range1723 1 1 1128 annotation2213988 1 Help:Barcodes range2213988 1 1129 1153 annotation7031 1 BBa_C0012 range7031 1 1 1128 annotation1722 1 LVA range1722 1 1090 1128 BBa_J61044 1 BBa_J61044 [LLO] Listeriolysin open reading frame 2007-02-20T12:00:00Z 2015-08-31T02:03:00Z bob bob false false _95_ 0 483 95 Not in stock false bob false John Anderson BBa_K177004 1 BBa_K177004 bistable_1_R 2009-02-18T12:00:00Z 2015-05-08T01:11:05Z right portion of bistable 1 switch false false _284_ 0 2582 9 Not in stock false false Michael Lower component2283191 1 BBa_B0015 component2283179 1 BBa_J61044 component2283182 1 BBa_K177007 component2283169 1 BBa_B0032 component2283164 1 BBa_R0051 component2283176 1 BBa_B0032 component2283184 1 BBa_Z0301 component2283178 1 BBa_I10001 component2283172 1 BBa_C0012 annotation2283184 1 BBa_Z0301 range2283184 1 7908 8627 annotation2283164 1 BBa_R0051 range2283164 1 1 49 annotation2283178 1 BBa_I10001 range2283178 1 1257 4220 annotation2283172 1 BBa_C0012 range2283172 1 77 1204 annotation2283182 1 BBa_K177007 range2283182 1 5764 7901 annotation2283169 1 BBa_B0032 range2283169 1 58 70 annotation2283176 1 BBa_B0032 range2283176 1 1238 1250 annotation2283179 1 BBa_J61044 range2283179 1 4227 5755 annotation2283191 1 BBa_B0015 range2283191 1 8636 8764 BBa_I10001 1 BBa_I10001 Invasin 2006-04-03T11:00:00Z 2015-08-31T04:07:29Z Invasin is a bacterial membrane protein (from Y.pseudotuberculosis) that binds tightly to beta-1-integrins found on the surface of many mamallian cells; interaction between invasin and beta-1-integrins induces the physical uptake of bacteria by mamallian cells via a Rac-1 mediated response (Wong & Isberg, 2005; PMID: 15694850) false false _89_45_47_11_12_21_8_48_46_1_10_43_ 0 52 89 Not in stock false false Chris Anderson (Voigt Lab) BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 BBa_Z0301 1 BBa_Z0301 Venus YFP - E. coli codon optimized 2006-06-20T11:00:00Z 2015-05-08T01:14:55Z Venus Sequence obtained from Prof. Miyawaki. Codon optimized from Gene Design by Boeke Lab. Brighter Variant than standard EYFP. false false _11_ 0 64 10 It's complicated false None really false Sriram Kosuri annotation1881736 1 gtc ...gtt -> gta ... gtt codon shuffle range1881736 1 4 39 BBa_K177004_sequence 1 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacaggaaagtactagatgatggttttccagccgatctctgagtttctgctgatccgtaacgctggtatgtctatgtacttcaacaaaatcatctctttcaacatcatctctcgtatcgttatctgcatcttcctgatctgcggtatgttcatggctggtgcttctgaaaaatacgacgctaacgctccgcagcaggttcagccgtactctgtttcttcttctgctttcgaaaacctgcacccgaacaacgaaatggaatcttctatcaacccgttctctgcttctgacaccgaacgtaacgctgctatcatcgaccgtgctaacaaagaacaggaaaccgaagctgttaacaaaatgatctctaccggtgctcgtctggctgcttctggtcgtgcttctgacgttgctcactctatggttggtgacgctgttaaccaggaaatcaaacagtggctgaaccgtttcggtaccgctcaggttaacctgaacttcgacaaaaacttctctctgaaagaatcttctctggactggctggctccgtggtacgactctgcttctttcctgttcttctctcagctgggtatccgtaacaaagactctcgtaacaccctgaacctgggtgttggtatccgtaccctggaaaacggttggctgtacggtctgaacaccttctacgacaacgacctgaccggtcacaaccaccgtatcggtctgggtgctgaagcttggaccgactacctccaactggctgctaacggttacttccgtctgaacggttggcactcttctcgtgacttctctgactacaaagaacgtccggctaccggtggtgacctgcgtgctaacgcttacctgccggctctgccgcagctgggtggtaaactgatgtacgaacagtacaccggtgaacgtgttgctctgttcggtaaagacaacctccaacgtaacccgtacgctgttaccgctggtatcaactacaccccggttccgctgctgaccgttggtgttgaccagcgtatgggtaaatcttctaaacacgaaacccagtggaacctccaaatgaactaccgtctgggtgaatctttccagtctcagctgtctccgtctgctgttgctggtacccgtctgctggctgaatctcgttacaacctggttgaccgtaacaacaacatcgttctggaataccagaaacagcaggttgttaaactgaccctgtctccggctaccatctctggtctgccgggtcaggtttaccaggttaacgctcaggttcagggtgcttctgctgttcgtgaaatcgtttggtctgacgctgaactgatcgctgctggtggtaccctgaccccgctgtctaccacccagttcaacctggttctgccgccgtacaaacgtaccgctcaggtttctcgtgttaccgacgacctgaccgctaacttctactctctgtctgctctggctgttgaccaccagggtaaccgttctaactctttcaccctgtctgttaccgttcagcagccgcagctgaccctgaccgctgctgttatcggtgacggtgctccggctaacggtaaaaccgctatcaccgttgagtttaccgttgctgacttcgaaggtaaaccgctggctggtcaggaagttgttatcaccaccaacaacggtgctctgccgaacaaaatcaccgaaaaaaccgacgctaacggtgttgctcgtatcgctctgaccaacaccaccgacggtgttaccgttgttaccgctgaagttgaaggtcagcgtcagtctgttgacacccacttcgttaaaggtaccatcgctgctgacaaatctaccctggctgctgttccgacctctatcatcgctgacggtctgatggcttctaccatcaccctggaactgaaagacacctacggtgacccgcaggctggtgctaacgttgctttcgacaccaccctgggtaacatgggtgttatcaccgaccacaacgacggtacctactctgctccgctgacctctaccaccctgggtgttgctaccgttaccgttaaagttgacggtgctgctttctctgttccgtctgttaccgttaacttcaccgctgacccgatcccggacgctggtcgttcttctttcaccgtttctaccccggacatcctggctgacggtaccatgtcttctaccctgtctttcgttccggttgacaaaaacggtcacttcatctctggtatgcagggtctgtctttcacccagaacggtgttccggtttctatctctccgatcaccgaacagccggactcttacaccgctaccgttgttggtaactctgttggtgacgttaccatcaccccgcaggttgacaccctgatcctgtctaccctccaaaaaaaaatctctctgttcccggttccgaccctgaccggtatcctggttaacggtcagaacttcgctaccgacaaaggtttcccgaaaaccatcttcaaaaacgctaccttccagctccaaatggacaacgacgttgctaacaacacccagtacgaatggtcttcttctttcaccccgaacgtttctgttaacgaccagggtcaggttaccatcacctaccagacctactctgaagttgctgttaccgctaaatctaaaaaattcccgtcttactctgtttcttaccgtttctacccgaaccgttggatctacgacggtggtcgttctctggtttcttctctggaagcttctcgtcagtgccagggttctgacatgtctgctgttctggaatcttctcgtgctaccaacggtacccgtgctccggacggtaccctgtggggtgaatggggttctctgaccgcttactcttctgactggcagtctggtgaatactgggttaaaaaaacctctaccgacttcgaaaccatgaacatggacaccggtgctctccaaccgggtccggcttacctggctttcccgctgtgcgctctgtctatctaataatactagatgaaggatgcatctgcattcaataaagaaaattcaatttcatccatggcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttacaaagatggaaatgaatatattgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagacattcaagttgtgaatgcaatttcgagcctaacctatccaggtgctctcgtaaaagcgaactcggaattagtagaaaatcaaccagatgttctccctgtaaaacgtgattcattaacactcagcattgatttgccaggtatgactaatcaagacaataaaatagttgtaaaaaatgccactaaatcaaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcgaaatttggtacagcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcgtatggccgtcaagtttatttgaaattatcaactaattcccatagtactaaagtaaaagctgcttttgatgctgccgtaagcggaaaatctgtctcaggtgatgtagaactaacaaatatcatcaaaaattcttccttcaaagccgtaatttacggaggttccgcaaaagatgaagttcaaatcatcgacggcaacctcggagacttacgcgatattttgaaaaaaggcgctacttttaatcgagaaacaccaggagttcccattgcttatacaacaaacttcctaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaattaacatcgatcactctggaggatacgttgctcaattcaacatttcttgggatgaagtaaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagcaagctagctcatttcacatcgtccatctatttgcctggtaacgcgagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacttaccacttgtgaaaaatagaaatatctccatctggggcaccacgctttatccgaaatatagtaataaagtagataatccaatcgaataataagaatttactagagctccaggattcaggtcaccaggtcgatgccgcagaagatgccagggaagctgattactaccttaatgaacaccttccggatatcgctattgtcgatttaggtctgccggatgaagacggcctttccttaatacgccgctggcgcagcagtgatgtttcactgccggttctggtgttaaccgcgcgcgaaggctggcaggataaagtcgaggttctcagctccggggccgatgactacgtgacgaagccattccacatcgaagaggtaatggcgcgtatgcaggcgttaatgcgccgtaatagcggtctggcctcccaggtgatcaacatcccgccgttccaggtggatctctcacgccgggaattatccgtcaatgaagaggtcatcaaactcacggcgttcgaatacaccattatggaaacgcttatccgtaacaacggtaaagtggtcagcaaagattcgctgatgcttcagctgtatccggatgcggaactgcgggaaagtcataccattgatgttctcatggggcgtctgcggaaaaaaatacaggcccagtatccgcacgatgtcattaccaccgtacgcggacaaggatatctttttgaattgcgctaatgaataaatttgctcgccattttctgccgctgtcgctgcgggttcgttttttgctggcgacagccggcgtcgtgctggtgctttctttggcatatggcatagtggcgctggtcggctatagcgtaagttttgataaaaccacctttcgtttgctgcgcggcgaaagcaacctgttttataccctcgccaaatgggaaaataataaaatcagcgttgagctgcctgaaaatctggacatgcaaagcccgaccatgacgctgatttacgatgaaacgggcaaattattatggacgcagcgcaacattccctggctgattaaaagcattcaaccggaatggttaaaaacgaacggcttccatgaaattgaaaccaacgtagacgccaccagcacgctgttgagcgaagaccattccgcgcaggaaaaactcaaagaagtacgtgaagatgacgatgatgccgagatgacccactcggtagcggtaaatatttatcctgccacggcgcggatgccgcagttaaccatcgtggtggtcgataccattccgatagaactaaaacgctcctatatggtgtggagctggttcgtatacgtgctggccgccaatttactgttagtcattcctttactgtggatcgccgcctggtggagcttacgccctatcgaggcgctggcgcgggaagtccgcgagcttgaagatcatcaccgcgaaatgctcaatccggagacgacgcgtgagctgaccagccttgtgcgcaaccttaatcaactgctcaaaagcgagcgtgaacgttataacaaataccgcacgaccctgaccgacctgacgcacagtttaaaaacgccgctcgcggttttgcagagtacgttacgctctttacgcaacgaaaagatgagcgtcagcaaagctgaaccggtgatgctggaacagatcagccggatttcccagcagatcggctattatctgcatcgcgccagtatgcgcggtagcggcgtgttgttaagccgcgaactgcatcccgtcgcgccgttgttagataacctgatttctgcgctaaataaagtttatcagcgtaaaggggtgaatatcagtatggatatttcaccagaaatcagttttgtcggcgagcaaaacgactttgtcgaagtgatgggcaacgtactggacaacgcttgtaaatattgtctggagtttgtcgagatttcggctcgccagaccgacgatcatttgcatattttcgtcgaagatgacggcccaggcattccccacagcaaacgttccctggtgtttgatcgcggtcagcgcgccgataccctacgaccaggacaaggcgtggggctggctgtcgcgcgcgagattacggaacaatacgccgggcagatcattgccagcgacagtctgctcggtggcgcccgtatggaggtcgtttttggccgacagcatcccacacagaaagaggaataaacatttctgtgcgttcttccacgcatctcgttaagcatccgttataatcggtgtcagatatactagatggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J61044_sequence 1 atgaaggatgcatctgcattcaataaagaaaattcaatttcatccatggcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttacaaagatggaaatgaatatattgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagacattcaagttgtgaatgcaatttcgagcctaacctatccaggtgctctcgtaaaagcgaactcggaattagtagaaaatcaaccagatgttctccctgtaaaacgtgattcattaacactcagcattgatttgccaggtatgactaatcaagacaataaaatagttgtaaaaaatgccactaaatcaaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcgaaatttggtacagcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcgtatggccgtcaagtttatttgaaattatcaactaattcccatagtactaaagtaaaagctgcttttgatgctgccgtaagcggaaaatctgtctcaggtgatgtagaactaacaaatatcatcaaaaattcttccttcaaagccgtaatttacggaggttccgcaaaagatgaagttcaaatcatcgacggcaacctcggagacttacgcgatattttgaaaaaaggcgctacttttaatcgagaaacaccaggagttcccattgcttatacaacaaacttcctaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaattaacatcgatcactctggaggatacgttgctcaattcaacatttcttgggatgaagtaaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagcaagctagctcatttcacatcgtccatctatttgcctggtaacgcgagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacttaccacttgtgaaaaatagaaatatctccatctggggcaccacgctttatccgaaatatagtaataaagtagataatccaatcgaataataagaatt BBa_R0051_sequence 1 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc BBa_Z0301_sequence 1 atggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataa BBa_B0032_sequence 1 tcacacaggaaag BBa_I10001_sequence 1 atgatggttttccagccgatctctgagtttctgctgatccgtaacgctggtatgtctatgtacttcaacaaaatcatctctttcaacatcatctctcgtatcgttatctgcatcttcctgatctgcggtatgttcatggctggtgcttctgaaaaatacgacgctaacgctccgcagcaggttcagccgtactctgtttcttcttctgctttcgaaaacctgcacccgaacaacgaaatggaatcttctatcaacccgttctctgcttctgacaccgaacgtaacgctgctatcatcgaccgtgctaacaaagaacaggaaaccgaagctgttaacaaaatgatctctaccggtgctcgtctggctgcttctggtcgtgcttctgacgttgctcactctatggttggtgacgctgttaaccaggaaatcaaacagtggctgaaccgtttcggtaccgctcaggttaacctgaacttcgacaaaaacttctctctgaaagaatcttctctggactggctggctccgtggtacgactctgcttctttcctgttcttctctcagctgggtatccgtaacaaagactctcgtaacaccctgaacctgggtgttggtatccgtaccctggaaaacggttggctgtacggtctgaacaccttctacgacaacgacctgaccggtcacaaccaccgtatcggtctgggtgctgaagcttggaccgactacctccaactggctgctaacggttacttccgtctgaacggttggcactcttctcgtgacttctctgactacaaagaacgtccggctaccggtggtgacctgcgtgctaacgcttacctgccggctctgccgcagctgggtggtaaactgatgtacgaacagtacaccggtgaacgtgttgctctgttcggtaaagacaacctccaacgtaacccgtacgctgttaccgctggtatcaactacaccccggttccgctgctgaccgttggtgttgaccagcgtatgggtaaatcttctaaacacgaaacccagtggaacctccaaatgaactaccgtctgggtgaatctttccagtctcagctgtctccgtctgctgttgctggtacccgtctgctggctgaatctcgttacaacctggttgaccgtaacaacaacatcgttctggaataccagaaacagcaggttgttaaactgaccctgtctccggctaccatctctggtctgccgggtcaggtttaccaggttaacgctcaggttcagggtgcttctgctgttcgtgaaatcgtttggtctgacgctgaactgatcgctgctggtggtaccctgaccccgctgtctaccacccagttcaacctggttctgccgccgtacaaacgtaccgctcaggtttctcgtgttaccgacgacctgaccgctaacttctactctctgtctgctctggctgttgaccaccagggtaaccgttctaactctttcaccctgtctgttaccgttcagcagccgcagctgaccctgaccgctgctgttatcggtgacggtgctccggctaacggtaaaaccgctatcaccgttgagtttaccgttgctgacttcgaaggtaaaccgctggctggtcaggaagttgttatcaccaccaacaacggtgctctgccgaacaaaatcaccgaaaaaaccgacgctaacggtgttgctcgtatcgctctgaccaacaccaccgacggtgttaccgttgttaccgctgaagttgaaggtcagcgtcagtctgttgacacccacttcgttaaaggtaccatcgctgctgacaaatctaccctggctgctgttccgacctctatcatcgctgacggtctgatggcttctaccatcaccctggaactgaaagacacctacggtgacccgcaggctggtgctaacgttgctttcgacaccaccctgggtaacatgggtgttatcaccgaccacaacgacggtacctactctgctccgctgacctctaccaccctgggtgttgctaccgttaccgttaaagttgacggtgctgctttctctgttccgtctgttaccgttaacttcaccgctgacccgatcccggacgctggtcgttcttctttcaccgtttctaccccggacatcctggctgacggtaccatgtcttctaccctgtctttcgttccggttgacaaaaacggtcacttcatctctggtatgcagggtctgtctttcacccagaacggtgttccggtttctatctctccgatcaccgaacagccggactcttacaccgctaccgttgttggtaactctgttggtgacgttaccatcaccccgcaggttgacaccctgatcctgtctaccctccaaaaaaaaatctctctgttcccggttccgaccctgaccggtatcctggttaacggtcagaacttcgctaccgacaaaggtttcccgaaaaccatcttcaaaaacgctaccttccagctccaaatggacaacgacgttgctaacaacacccagtacgaatggtcttcttctttcaccccgaacgtttctgttaacgaccagggtcaggttaccatcacctaccagacctactctgaagttgctgttaccgctaaatctaaaaaattcccgtcttactctgtttcttaccgtttctacccgaaccgttggatctacgacggtggtcgttctctggtttcttctctggaagcttctcgtcagtgccagggttctgacatgtctgctgttctggaatcttctcgtgctaccaacggtacccgtgctccggacggtaccctgtggggtgaatggggttctctgaccgcttactcttctgactggcagtctggtgaatactgggttaaaaaaacctctaccgacttcgaaaccatgaacatggacaccggtgctctccaaccgggtccggcttacctggctttcccgctgtgcgctctgtctatctaataa BBa_C0012_sequence 1 atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc BBa_K177007_sequence 1 ctccaggattcaggtcaccaggtcgatgccgcagaagatgccagggaagctgattactaccttaatgaacaccttccggatatcgctattgtcgatttaggtctgccggatgaagacggcctttccttaatacgccgctggcgcagcagtgatgtttcactgccggttctggtgttaaccgcgcgcgaaggctggcaggataaagtcgaggttctcagctccggggccgatgactacgtgacgaagccattccacatcgaagaggtaatggcgcgtatgcaggcgttaatgcgccgtaatagcggtctggcctcccaggtgatcaacatcccgccgttccaggtggatctctcacgccgggaattatccgtcaatgaagaggtcatcaaactcacggcgttcgaatacaccattatggaaacgcttatccgtaacaacggtaaagtggtcagcaaagattcgctgatgcttcagctgtatccggatgcggaactgcgggaaagtcataccattgatgttctcatggggcgtctgcggaaaaaaatacaggcccagtatccgcacgatgtcattaccaccgtacgcggacaaggatatctttttgaattgcgctaatgaataaatttgctcgccattttctgccgctgtcgctgcgggttcgttttttgctggcgacagccggcgtcgtgctggtgctttctttggcatatggcatagtggcgctggtcggctatagcgtaagttttgataaaaccacctttcgtttgctgcgcggcgaaagcaacctgttttataccctcgccaaatgggaaaataataaaatcagcgttgagctgcctgaaaatctggacatgcaaagcccgaccatgacgctgatttacgatgaaacgggcaaattattatggacgcagcgcaacattccctggctgattaaaagcattcaaccggaatggttaaaaacgaacggcttccatgaaattgaaaccaacgtagacgccaccagcacgctgttgagcgaagaccattccgcgcaggaaaaactcaaagaagtacgtgaagatgacgatgatgccgagatgacccactcggtagcggtaaatatttatcctgccacggcgcggatgccgcagttaaccatcgtggtggtcgataccattccgatagaactaaaacgctcctatatggtgtggagctggttcgtatacgtgctggccgccaatttactgttagtcattcctttactgtggatcgccgcctggtggagcttacgccctatcgaggcgctggcgcgggaagtccgcgagcttgaagatcatcaccgcgaaatgctcaatccggagacgacgcgtgagctgaccagccttgtgcgcaaccttaatcaactgctcaaaagcgagcgtgaacgttataacaaataccgcacgaccctgaccgacctgacgcacagtttaaaaacgccgctcgcggttttgcagagtacgttacgctctttacgcaacgaaaagatgagcgtcagcaaagctgaaccggtgatgctggaacagatcagccggatttcccagcagatcggctattatctgcatcgcgccagtatgcgcggtagcggcgtgttgttaagccgcgaactgcatcccgtcgcgccgttgttagataacctgatttctgcgctaaataaagtttatcagcgtaaaggggtgaatatcagtatggatatttcaccagaaatcagttttgtcggcgagcaaaacgactttgtcgaagtgatgggcaacgtactggacaacgcttgtaaatattgtctggagtttgtcgagatttcggctcgccagaccgacgatcatttgcatattttcgtcgaagatgacggcccaggcattccccacagcaaacgttccctggtgtttgatcgcggtcagcgcgccgataccctacgaccaggacaaggcgtggggctggctgtcgcgcgcgagattacggaacaatacgccgggcagatcattgccagcgacagtctgctcggtggcgcccgtatggaggtcgtttttggccgacagcatcccacacagaaagaggaataaacatttctgtgcgttcttccacgcatctcgttaagcatccgttataatcggtgtcagata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z