##gff-version 3
##sequence-region BBa_K177004 1 8764
BBa_K177004	.	terminator	8636	8764	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K177004	.	terminator	8636	8715	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K177004	.	stem_loop	8647	8690	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K177004	.	engineered_region	8636	8715	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177004	.	terminator	8724	8764	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K177004	.	polyA_site	8751	8764	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177004	.	stem_loop	8731	8750	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177004	.	engineered_region	8724	8764	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177004	.	stop_codon	8757	8757	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177004	.	ribosome_entry_site	1238	1250	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K177004	.	engineered_region	1238	1250	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177004	.	ribosome_entry_site	1238	1250	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177004	.	ribosome_entry_site	1244	1247	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177004	.	engineered_region	7908	8627	.	+	0	ID=BBa_Z0301;Name=BBa_Z0301
BBa_K177004	.	point_mutation	7911	7946	.	+	0	ID=annotation1881736;Name=gtc ...gtt -> gta ... gtt codon shuffle;Parent=BBa_Z0301
BBa_K177004	.	CDS	77	1204	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K177004	.	engineered_region	77	1204	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K177004	.	CDS	77	1204	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K177004	.	engineered_tag	1205	1229	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K177004	.	tag	1166	1204	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K177004	.	CDS	1257	4220	.	+	0	ID=BBa_I10001;Name=BBa_I10001
BBa_K177004	.	CDS	5764	7901	.	+	0	ID=BBa_K177007;Name=BBa_K177007
BBa_K177004	.	CDS	5764	6438	.	+	0	ID=annotation2000913;Name=PhoP;Parent=BBa_K177007
BBa_K177004	.	sequence_feature	6438	7901	.	+	0	ID=annotation2000914;Name=PhoQ;Parent=BBa_K177007
BBa_K177004	.	ribosome_entry_site	58	70	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K177004	.	engineered_region	58	70	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177004	.	ribosome_entry_site	58	70	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177004	.	ribosome_entry_site	64	67	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177004	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K177004	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K177004	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K177004	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K177004	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K177004	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K177004	.	CDS	4227	5755	.	+	0	ID=BBa_J61044;Name=BBa_J61044
>BBa_K177004
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatg
gtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtga
accaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccg
cgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcg
caaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcg
tcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatga
ccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatc
aacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcg
cgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatca
aattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgag
ggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggc
tgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccac
catcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaag
ggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcg
cgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaacta
cgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacaggaaagtactagatgatggt
tttccagccgatctctgagtttctgctgatccgtaacgctggtatgtctatgtacttcaacaaaatcatctctttcaac
atcatctctcgtatcgttatctgcatcttcctgatctgcggtatgttcatggctggtgcttctgaaaaatacgacgcta
acgctccgcagcaggttcagccgtactctgtttcttcttctgctttcgaaaacctgcacccgaacaacgaaatggaatc
ttctatcaacccgttctctgcttctgacaccgaacgtaacgctgctatcatcgaccgtgctaacaaagaacaggaaacc
gaagctgttaacaaaatgatctctaccggtgctcgtctggctgcttctggtcgtgcttctgacgttgctcactctatgg
ttggtgacgctgttaaccaggaaatcaaacagtggctgaaccgtttcggtaccgctcaggttaacctgaacttcgacaa
aaacttctctctgaaagaatcttctctggactggctggctccgtggtacgactctgcttctttcctgttcttctctcag
ctgggtatccgtaacaaagactctcgtaacaccctgaacctgggtgttggtatccgtaccctggaaaacggttggctgt
acggtctgaacaccttctacgacaacgacctgaccggtcacaaccaccgtatcggtctgggtgctgaagcttggaccga
ctacctccaactggctgctaacggttacttccgtctgaacggttggcactcttctcgtgacttctctgactacaaagaa
cgtccggctaccggtggtgacctgcgtgctaacgcttacctgccggctctgccgcagctgggtggtaaactgatgtacg
aacagtacaccggtgaacgtgttgctctgttcggtaaagacaacctccaacgtaacccgtacgctgttaccgctggtat
caactacaccccggttccgctgctgaccgttggtgttgaccagcgtatgggtaaatcttctaaacacgaaacccagtgg
aacctccaaatgaactaccgtctgggtgaatctttccagtctcagctgtctccgtctgctgttgctggtacccgtctgc
tggctgaatctcgttacaacctggttgaccgtaacaacaacatcgttctggaataccagaaacagcaggttgttaaact
gaccctgtctccggctaccatctctggtctgccgggtcaggtttaccaggttaacgctcaggttcagggtgcttctgct
gttcgtgaaatcgtttggtctgacgctgaactgatcgctgctggtggtaccctgaccccgctgtctaccacccagttca
acctggttctgccgccgtacaaacgtaccgctcaggtttctcgtgttaccgacgacctgaccgctaacttctactctct
gtctgctctggctgttgaccaccagggtaaccgttctaactctttcaccctgtctgttaccgttcagcagccgcagctg
accctgaccgctgctgttatcggtgacggtgctccggctaacggtaaaaccgctatcaccgttgagtttaccgttgctg
acttcgaaggtaaaccgctggctggtcaggaagttgttatcaccaccaacaacggtgctctgccgaacaaaatcaccga
aaaaaccgacgctaacggtgttgctcgtatcgctctgaccaacaccaccgacggtgttaccgttgttaccgctgaagtt
gaaggtcagcgtcagtctgttgacacccacttcgttaaaggtaccatcgctgctgacaaatctaccctggctgctgttc
cgacctctatcatcgctgacggtctgatggcttctaccatcaccctggaactgaaagacacctacggtgacccgcaggc
tggtgctaacgttgctttcgacaccaccctgggtaacatgggtgttatcaccgaccacaacgacggtacctactctgct
ccgctgacctctaccaccctgggtgttgctaccgttaccgttaaagttgacggtgctgctttctctgttccgtctgtta
ccgttaacttcaccgctgacccgatcccggacgctggtcgttcttctttcaccgtttctaccccggacatcctggctga
cggtaccatgtcttctaccctgtctttcgttccggttgacaaaaacggtcacttcatctctggtatgcagggtctgtct
ttcacccagaacggtgttccggtttctatctctccgatcaccgaacagccggactcttacaccgctaccgttgttggta
actctgttggtgacgttaccatcaccccgcaggttgacaccctgatcctgtctaccctccaaaaaaaaatctctctgtt
cccggttccgaccctgaccggtatcctggttaacggtcagaacttcgctaccgacaaaggtttcccgaaaaccatcttc
aaaaacgctaccttccagctccaaatggacaacgacgttgctaacaacacccagtacgaatggtcttcttctttcaccc
cgaacgtttctgttaacgaccagggtcaggttaccatcacctaccagacctactctgaagttgctgttaccgctaaatc
taaaaaattcccgtcttactctgtttcttaccgtttctacccgaaccgttggatctacgacggtggtcgttctctggtt
tcttctctggaagcttctcgtcagtgccagggttctgacatgtctgctgttctggaatcttctcgtgctaccaacggta
cccgtgctccggacggtaccctgtggggtgaatggggttctctgaccgcttactcttctgactggcagtctggtgaata
ctgggttaaaaaaacctctaccgacttcgaaaccatgaacatggacaccggtgctctccaaccgggtccggcttacctg
gctttcccgctgtgcgctctgtctatctaataatactagatgaaggatgcatctgcattcaataaagaaaattcaattt
catccatggcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataa
gtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaaga
aaaggttacaaagatggaaatgaatatattgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagacattc
aagttgtgaatgcaatttcgagcctaacctatccaggtgctctcgtaaaagcgaactcggaattagtagaaaatcaacc
agatgttctccctgtaaaacgtgattcattaacactcagcattgatttgccaggtatgactaatcaagacaataaaata
gttgtaaaaaatgccactaaatcaaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctc
aagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcgaaatt
tggtacagcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaa
gtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctg
ttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcgtatggccg
tcaagtttatttgaaattatcaactaattcccatagtactaaagtaaaagctgcttttgatgctgccgtaagcggaaaa
tctgtctcaggtgatgtagaactaacaaatatcatcaaaaattcttccttcaaagccgtaatttacggaggttccgcaa
aagatgaagttcaaatcatcgacggcaacctcggagacttacgcgatattttgaaaaaaggcgctacttttaatcgaga
aacaccaggagttcccattgcttatacaacaaacttcctaaaagacaatgaattagctgttattaaaaacaactcagaa
tatattgaaacaacttcaaaagcttatacagatggaaaaattaacatcgatcactctggaggatacgttgctcaattca
acatttcttgggatgaagtaaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataa
aagcaagctagctcatttcacatcgtccatctatttgcctggtaacgcgagaaatattaatgtttacgctaaagaatgc
actggtttagcttgggaatggtggagaacggtaattgatgaccggaacttaccacttgtgaaaaatagaaatatctcca
tctggggcaccacgctttatccgaaatatagtaataaagtagataatccaatcgaataataagaatttactagagctcc
aggattcaggtcaccaggtcgatgccgcagaagatgccagggaagctgattactaccttaatgaacaccttccggatat
cgctattgtcgatttaggtctgccggatgaagacggcctttccttaatacgccgctggcgcagcagtgatgtttcactg
ccggttctggtgttaaccgcgcgcgaaggctggcaggataaagtcgaggttctcagctccggggccgatgactacgtga
cgaagccattccacatcgaagaggtaatggcgcgtatgcaggcgttaatgcgccgtaatagcggtctggcctcccaggt
gatcaacatcccgccgttccaggtggatctctcacgccgggaattatccgtcaatgaagaggtcatcaaactcacggcg
ttcgaatacaccattatggaaacgcttatccgtaacaacggtaaagtggtcagcaaagattcgctgatgcttcagctgt
atccggatgcggaactgcgggaaagtcataccattgatgttctcatggggcgtctgcggaaaaaaatacaggcccagta
tccgcacgatgtcattaccaccgtacgcggacaaggatatctttttgaattgcgctaatgaataaatttgctcgccatt
ttctgccgctgtcgctgcgggttcgttttttgctggcgacagccggcgtcgtgctggtgctttctttggcatatggcat
agtggcgctggtcggctatagcgtaagttttgataaaaccacctttcgtttgctgcgcggcgaaagcaacctgttttat
accctcgccaaatgggaaaataataaaatcagcgttgagctgcctgaaaatctggacatgcaaagcccgaccatgacgc
tgatttacgatgaaacgggcaaattattatggacgcagcgcaacattccctggctgattaaaagcattcaaccggaatg
gttaaaaacgaacggcttccatgaaattgaaaccaacgtagacgccaccagcacgctgttgagcgaagaccattccgcg
caggaaaaactcaaagaagtacgtgaagatgacgatgatgccgagatgacccactcggtagcggtaaatatttatcctg
ccacggcgcggatgccgcagttaaccatcgtggtggtcgataccattccgatagaactaaaacgctcctatatggtgtg
gagctggttcgtatacgtgctggccgccaatttactgttagtcattcctttactgtggatcgccgcctggtggagctta
cgccctatcgaggcgctggcgcgggaagtccgcgagcttgaagatcatcaccgcgaaatgctcaatccggagacgacgc
gtgagctgaccagccttgtgcgcaaccttaatcaactgctcaaaagcgagcgtgaacgttataacaaataccgcacgac
cctgaccgacctgacgcacagtttaaaaacgccgctcgcggttttgcagagtacgttacgctctttacgcaacgaaaag
atgagcgtcagcaaagctgaaccggtgatgctggaacagatcagccggatttcccagcagatcggctattatctgcatc
gcgccagtatgcgcggtagcggcgtgttgttaagccgcgaactgcatcccgtcgcgccgttgttagataacctgatttc
tgcgctaaataaagtttatcagcgtaaaggggtgaatatcagtatggatatttcaccagaaatcagttttgtcggcgag
caaaacgactttgtcgaagtgatgggcaacgtactggacaacgcttgtaaatattgtctggagtttgtcgagatttcgg
ctcgccagaccgacgatcatttgcatattttcgtcgaagatgacggcccaggcattccccacagcaaacgttccctggt
gtttgatcgcggtcagcgcgccgataccctacgaccaggacaaggcgtggggctggctgtcgcgcgcgagattacggaa
caatacgccgggcagatcattgccagcgacagtctgctcggtggcgcccgtatggaggtcgtttttggccgacagcatc
ccacacagaaagaggaataaacatttctgtgcgttcttccacgcatctcgttaagcatccgttataatcggtgtcagat
atactagatggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaac
ggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcacca
ccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccgga
ccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagac
gacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcg
acttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctga
caaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccac
taccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgt
ctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatgga
cgaactgtacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata