LOCUS BBa_K177005 9748 bp DNA linear 14-Jun-2024 DEFINITION Bistable_1 ACCESSION BBa_K177005 VERSION BBa_K177005.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 RBS 46..58 /label=RBS-3\Weak RBS 52..55 /label=RBS misc_feature 46..58 /label=BBa_B0032 RBS 46..58 /label=BBa_B0032 CDS 68..775 /label=cI lambda misc_feature 776..808 /label=LVA misc_feature 815..839 /label=Help:Barcodes CDS 65..839 /label=BBa_C0051 stem_loop 859..902 /label=stem_loop misc_feature 848..927 /label=BBa_B0010 terminator 848..927 /label=BBa_B0010 stem_loop 943..962 /label=T7 TE misc_feature 969..969 /label=stop polyA_site 963..976 /label=polya misc_feature 936..976 /label=BBa_B0012 terminator 936..976 /label=BBa_B0012 terminator 848..976 /label=BBa_B0015 misc_feature 1..976 /label=BBa_K177000 promoter 1022..1027 /label=-10 promoter 999..1004 /label=-35 misc_feature 1009..1025 /label=OR1 misc_feature 985..1001 /label=OR2 misc_feature 985..1033 /label=BBa_R0051 promoter 985..1033 /label=BBa_R0051 RBS 1042..1054 /label=RBS-3\Weak RBS 1048..1051 /label=RBS misc_feature 1042..1054 /label=BBa_B0032 RBS 1042..1054 /label=BBa_B0032 misc_feature 2150..2188 /label=LVA CDS 1061..2188 /label=lacI-LVA misc_feature 1061..2188 /label=BBa_C0012 misc_feature 2189..2213 /label=Help:Barcodes CDS 1061..2188 /label=BBa_C0012 RBS 2222..2234 /label=RBS-3\Weak RBS 2228..2231 /label=RBS misc_feature 2222..2234 /label=BBa_B0032 RBS 2222..2234 /label=BBa_B0032 CDS 2241..5204 /label=BBa_I10001 CDS 5211..6739 /label=BBa_J61044 CDS 6748..7422 /label=PhoP misc_feature 7422..8885 /label=PhoQ CDS 6748..8885 /label=BBa_K177007 misc_feature 8895..8930 /label=gtc ...gtt -> gta ... gtt codon shuffle misc_feature 8892..9611 /label=BBa_Z0301 stem_loop 9631..9674 /label=stem_loop misc_feature 9620..9699 /label=BBa_B0010 terminator 9620..9699 /label=BBa_B0010 stem_loop 9715..9734 /label=T7 TE misc_feature 9741..9741 /label=stop polyA_site 9735..9748 /label=polya misc_feature 9708..9748 /label=BBa_B0012 terminator 9708..9748 /label=BBa_B0012 terminator 9620..9748 /label=BBa_B0015 misc_feature 985..9748 /label=BBa_K177004 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagtcaca caggaaagta 61 ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct 121 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga 181 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa 241 tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc 301 ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag 361 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct 421 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga 481 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc 541 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga 601 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag 661 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag 721 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc 781 aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct 841 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 901 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 961 cctttctgcg tttatatact agagtaacac cgtgcgtgtt gactatttta cctctggcgg 1021 tgataatggt tgctactaga gtcacacagg aaagtactag atggtgaatg tgaaaccagt 1081 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt 1141 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga 1201 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat 1261 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa 1321 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt 1381 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat 1441 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc 1501 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga 1561 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct 1621 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata 1681 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc 1741 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt 1801 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt 1861 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc 1921 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt 1981 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt 2041 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 2101 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga 2161 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga 2221 gtcacacagg aaagtactag atgatggttt tccagccgat ctctgagttt ctgctgatcc 2281 gtaacgctgg tatgtctatg tacttcaaca aaatcatctc tttcaacatc atctctcgta 2341 tcgttatctg catcttcctg atctgcggta tgttcatggc tggtgcttct gaaaaatacg 2401 acgctaacgc tccgcagcag gttcagccgt actctgtttc ttcttctgct ttcgaaaacc 2461 tgcacccgaa caacgaaatg gaatcttcta tcaacccgtt ctctgcttct gacaccgaac 2521 gtaacgctgc tatcatcgac cgtgctaaca aagaacagga aaccgaagct gttaacaaaa 2581 tgatctctac cggtgctcgt ctggctgctt ctggtcgtgc ttctgacgtt gctcactcta 2641 tggttggtga cgctgttaac caggaaatca aacagtggct gaaccgtttc ggtaccgctc 2701 aggttaacct gaacttcgac aaaaacttct ctctgaaaga atcttctctg gactggctgg 2761 ctccgtggta cgactctgct tctttcctgt tcttctctca gctgggtatc cgtaacaaag 2821 actctcgtaa caccctgaac ctgggtgttg gtatccgtac cctggaaaac ggttggctgt 2881 acggtctgaa caccttctac gacaacgacc tgaccggtca caaccaccgt atcggtctgg 2941 gtgctgaagc ttggaccgac tacctccaac tggctgctaa cggttacttc cgtctgaacg 3001 gttggcactc ttctcgtgac ttctctgact acaaagaacg tccggctacc ggtggtgacc 3061 tgcgtgctaa cgcttacctg ccggctctgc cgcagctggg tggtaaactg atgtacgaac 3121 agtacaccgg tgaacgtgtt gctctgttcg gtaaagacaa cctccaacgt aacccgtacg 3181 ctgttaccgc tggtatcaac tacaccccgg ttccgctgct gaccgttggt gttgaccagc 3241 gtatgggtaa atcttctaaa cacgaaaccc agtggaacct ccaaatgaac taccgtctgg 3301 gtgaatcttt ccagtctcag ctgtctccgt ctgctgttgc tggtacccgt ctgctggctg 3361 aatctcgtta caacctggtt gaccgtaaca acaacatcgt tctggaatac cagaaacagc 3421 aggttgttaa actgaccctg tctccggcta ccatctctgg tctgccgggt caggtttacc 3481 aggttaacgc tcaggttcag ggtgcttctg ctgttcgtga aatcgtttgg tctgacgctg 3541 aactgatcgc tgctggtggt accctgaccc cgctgtctac cacccagttc aacctggttc 3601 tgccgccgta caaacgtacc gctcaggttt ctcgtgttac cgacgacctg accgctaact 3661 tctactctct gtctgctctg gctgttgacc accagggtaa ccgttctaac tctttcaccc 3721 tgtctgttac cgttcagcag ccgcagctga ccctgaccgc tgctgttatc ggtgacggtg 3781 ctccggctaa cggtaaaacc gctatcaccg ttgagtttac cgttgctgac ttcgaaggta 3841 aaccgctggc tggtcaggaa gttgttatca ccaccaacaa cggtgctctg ccgaacaaaa 3901 tcaccgaaaa aaccgacgct aacggtgttg ctcgtatcgc tctgaccaac accaccgacg 3961 gtgttaccgt tgttaccgct gaagttgaag gtcagcgtca gtctgttgac acccacttcg 4021 ttaaaggtac catcgctgct gacaaatcta ccctggctgc tgttccgacc tctatcatcg 4081 ctgacggtct gatggcttct accatcaccc tggaactgaa agacacctac ggtgacccgc 4141 aggctggtgc taacgttgct ttcgacacca ccctgggtaa catgggtgtt atcaccgacc 4201 acaacgacgg tacctactct gctccgctga cctctaccac cctgggtgtt gctaccgtta 4261 ccgttaaagt tgacggtgct gctttctctg ttccgtctgt taccgttaac ttcaccgctg 4321 acccgatccc ggacgctggt cgttcttctt tcaccgtttc taccccggac atcctggctg 4381 acggtaccat gtcttctacc ctgtctttcg ttccggttga caaaaacggt cacttcatct 4441 ctggtatgca gggtctgtct ttcacccaga acggtgttcc ggtttctatc tctccgatca 4501 ccgaacagcc ggactcttac accgctaccg ttgttggtaa ctctgttggt gacgttacca 4561 tcaccccgca ggttgacacc ctgatcctgt ctaccctcca aaaaaaaatc tctctgttcc 4621 cggttccgac cctgaccggt atcctggtta acggtcagaa cttcgctacc gacaaaggtt 4681 tcccgaaaac catcttcaaa aacgctacct tccagctcca aatggacaac gacgttgcta 4741 acaacaccca gtacgaatgg tcttcttctt tcaccccgaa cgtttctgtt aacgaccagg 4801 gtcaggttac catcacctac cagacctact ctgaagttgc tgttaccgct aaatctaaaa 4861 aattcccgtc ttactctgtt tcttaccgtt tctacccgaa ccgttggatc tacgacggtg 4921 gtcgttctct ggtttcttct ctggaagctt ctcgtcagtg ccagggttct gacatgtctg 4981 ctgttctgga atcttctcgt gctaccaacg gtacccgtgc tccggacggt accctgtggg 5041 gtgaatgggg ttctctgacc gcttactctt ctgactggca gtctggtgaa tactgggtta 5101 aaaaaacctc taccgacttc gaaaccatga acatggacac cggtgctctc caaccgggtc 5161 cggcttacct ggctttcccg ctgtgcgctc tgtctatcta ataatactag atgaaggatg 5221 catctgcatt caataaagaa aattcaattt catccatggc accaccagca tctccgcctg 5281 caagtcctaa gacgccaatc gaaaagaaac acgcggatga aatcgataag tatatacaag 5341 gattggatta caataaaaac aatgtattag tataccacgg agatgcagtg acaaatgtgc 5401 cgccaagaaa aggttacaaa gatggaaatg aatatattgt tgtggagaaa aagaagaaat 5461 ccatcaatca aaataatgca gacattcaag ttgtgaatgc aatttcgagc ctaacctatc 5521 caggtgctct cgtaaaagcg aactcggaat tagtagaaaa tcaaccagat gttctccctg 5581 taaaacgtga ttcattaaca ctcagcattg atttgccagg tatgactaat caagacaata 5641 aaatagttgt aaaaaatgcc actaaatcaa acgttaacaa cgcagtaaat acattagtgg 5701 aaagatggaa tgaaaaatat gctcaagctt atccaaatgt aagtgcaaaa attgattatg 5761 atgacgaaat ggcttacagt gaatcacaat taattgcgaa atttggtaca gcatttaaag 5821 ctgtaaataa tagcttgaat gtaaacttcg gcgcaatcag tgaagggaaa atgcaagaag 5881 aagtcattag ttttaaacaa atttactata acgtgaatgt taatgaacct acaagacctt 5941 ccagattttt cggcaaagct gttactaaag agcagttgca agcgcttgga gtgaatgcag 6001 aaaatcctcc tgcatatatc tcaagtgtgg cgtatggccg tcaagtttat ttgaaattat 6061 caactaattc ccatagtact aaagtaaaag ctgcttttga tgctgccgta agcggaaaat 6121 ctgtctcagg tgatgtagaa ctaacaaata tcatcaaaaa ttcttccttc aaagccgtaa 6181 tttacggagg ttccgcaaaa gatgaagttc aaatcatcga cggcaacctc ggagacttac 6241 gcgatatttt gaaaaaaggc gctactttta atcgagaaac accaggagtt cccattgctt 6301 atacaacaaa cttcctaaaa gacaatgaat tagctgttat taaaaacaac tcagaatata 6361 ttgaaacaac ttcaaaagct tatacagatg gaaaaattaa catcgatcac tctggaggat 6421 acgttgctca attcaacatt tcttgggatg aagtaaatta tgatcctgaa ggtaacgaaa 6481 ttgttcaaca taaaaactgg agcgaaaaca ataaaagcaa gctagctcat ttcacatcgt 6541 ccatctattt gcctggtaac gcgagaaata ttaatgttta cgctaaagaa tgcactggtt 6601 tagcttggga atggtggaga acggtaattg atgaccggaa cttaccactt gtgaaaaata 6661 gaaatatctc catctggggc accacgcttt atccgaaata tagtaataaa gtagataatc 6721 caatcgaata ataagaattt actagagctc caggattcag gtcaccaggt cgatgccgca 6781 gaagatgcca gggaagctga ttactacctt aatgaacacc ttccggatat cgctattgtc 6841 gatttaggtc tgccggatga agacggcctt tccttaatac gccgctggcg cagcagtgat 6901 gtttcactgc cggttctggt gttaaccgcg cgcgaaggct ggcaggataa agtcgaggtt 6961 ctcagctccg gggccgatga ctacgtgacg aagccattcc acatcgaaga ggtaatggcg 7021 cgtatgcagg cgttaatgcg ccgtaatagc ggtctggcct cccaggtgat caacatcccg 7081 ccgttccagg tggatctctc acgccgggaa ttatccgtca atgaagaggt catcaaactc 7141 acggcgttcg aatacaccat tatggaaacg cttatccgta acaacggtaa agtggtcagc 7201 aaagattcgc tgatgcttca gctgtatccg gatgcggaac tgcgggaaag tcataccatt 7261 gatgttctca tggggcgtct gcggaaaaaa atacaggccc agtatccgca cgatgtcatt 7321 accaccgtac gcggacaagg atatcttttt gaattgcgct aatgaataaa tttgctcgcc 7381 attttctgcc gctgtcgctg cgggttcgtt ttttgctggc gacagccggc gtcgtgctgg 7441 tgctttcttt ggcatatggc atagtggcgc tggtcggcta tagcgtaagt tttgataaaa 7501 ccacctttcg tttgctgcgc ggcgaaagca acctgtttta taccctcgcc aaatgggaaa 7561 ataataaaat cagcgttgag ctgcctgaaa atctggacat gcaaagcccg accatgacgc 7621 tgatttacga tgaaacgggc aaattattat ggacgcagcg caacattccc tggctgatta 7681 aaagcattca accggaatgg ttaaaaacga acggcttcca tgaaattgaa accaacgtag 7741 acgccaccag cacgctgttg agcgaagacc attccgcgca ggaaaaactc aaagaagtac 7801 gtgaagatga cgatgatgcc gagatgaccc actcggtagc ggtaaatatt tatcctgcca 7861 cggcgcggat gccgcagtta accatcgtgg tggtcgatac cattccgata gaactaaaac 7921 gctcctatat ggtgtggagc tggttcgtat acgtgctggc cgccaattta ctgttagtca 7981 ttcctttact gtggatcgcc gcctggtgga gcttacgccc tatcgaggcg ctggcgcggg 8041 aagtccgcga gcttgaagat catcaccgcg aaatgctcaa tccggagacg acgcgtgagc 8101 tgaccagcct tgtgcgcaac cttaatcaac tgctcaaaag cgagcgtgaa cgttataaca 8161 aataccgcac gaccctgacc gacctgacgc acagtttaaa aacgccgctc gcggttttgc 8221 agagtacgtt acgctcttta cgcaacgaaa agatgagcgt cagcaaagct gaaccggtga 8281 tgctggaaca gatcagccgg atttcccagc agatcggcta ttatctgcat cgcgccagta 8341 tgcgcggtag cggcgtgttg ttaagccgcg aactgcatcc cgtcgcgccg ttgttagata 8401 acctgatttc tgcgctaaat aaagtttatc agcgtaaagg ggtgaatatc agtatggata 8461 tttcaccaga aatcagtttt gtcggcgagc aaaacgactt tgtcgaagtg atgggcaacg 8521 tactggacaa cgcttgtaaa tattgtctgg agtttgtcga gatttcggct cgccagaccg 8581 acgatcattt gcatattttc gtcgaagatg acggcccagg cattccccac agcaaacgtt 8641 ccctggtgtt tgatcgcggt cagcgcgccg ataccctacg accaggacaa ggcgtggggc 8701 tggctgtcgc gcgcgagatt acggaacaat acgccgggca gatcattgcc agcgacagtc 8761 tgctcggtgg cgcccgtatg gaggtcgttt ttggccgaca gcatcccaca cagaaagagg 8821 aataaacatt tctgtgcgtt cttccacgca tctcgttaag catccgttat aatcggtgtc 8881 agatatacta gatggtaagc aaaggagagg aattatttac aggagttgtt ccgatcctgg 8941 ttgaactgga cggtgacgtt aacggtcaca aattctctgt ttctggtgaa ggtgaaggtg 9001 acgctaccta cggtaaactg accctgaaac tgatctgcac caccggtaaa ctgccggttc 9061 cgtggccgac cctcgtaact accctgggtt acggtctcca atgcttcgct cgttacccgg 9121 accacatgaa acagcacgac ttcttcaaat ctgctatgcc ggaaggttac gttcaggaac 9181 gtaccatctt cttcaaagac gacggtaact acaaaacccg tgctgaagtt aaattcgaag 9241 gtgacaccct ggttaaccgt atcgaactga aaggtatcga cttcaaagaa gacggtaaca 9301 tcctgggtca caaactggaa tacaactaca actctcacaa cgtttacatc accgctgaca 9361 aacagaaaaa cggtatcaaa gctaacttca aaatccgtca caacatcgaa gacggtggtg 9421 ttcagctggc tgaccactac cagcagaaca ccccaattgg tgatggccca gttctgctgc 9481 cggacaacca ctacctgtct taccagtctg ctctgtctaa agacccgaac gaaaaacgtg 9541 accacatggt tctgctggag tttgttaccg ctgctggtat caccctgggt atggacgaac 9601 tgtacaaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 9661 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 9721 ccttcgggtg ggcctttctg cgtttata //