##gff-version 3
##sequence-region BBa_K177005 1 9748
BBa_K177005	.	engineered_region	985	9748	.	+	0	ID=BBa_K177004;Name=BBa_K177004
BBa_K177005	.	CDS	6748	8885	.	+	0	ID=BBa_K177007;Name=BBa_K177007;Parent=BBa_K177004
BBa_K177005	.	CDS	6748	7422	.	+	0	ID=annotation2000913;Name=PhoP;Parent=BBa_K177007
BBa_K177005	.	sequence_feature	7422	8885	.	+	0	ID=annotation2000914;Name=PhoQ;Parent=BBa_K177007
BBa_K177005	.	CDS	2241	5204	.	+	0	ID=BBa_I10001;Name=BBa_I10001;Parent=BBa_K177004
BBa_K177005	.	promoter	985	1033	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K177004
BBa_K177005	.	promoter	1022	1027	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K177005	.	non_covalent_binding_site	1009	1025	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K177005	.	engineered_region	985	1033	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K177005	.	promoter	999	1004	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K177005	.	non_covalent_binding_site	985	1001	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K177005	.	engineered_region	8892	9611	.	+	0	ID=BBa_Z0301;Name=BBa_Z0301;Parent=BBa_K177004
BBa_K177005	.	point_mutation	8895	8930	.	+	0	ID=annotation1881736;Name=gtc ...gtt -> gta ... gtt codon shuffle;Parent=BBa_Z0301
BBa_K177005	.	terminator	9620	9748	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K177004
BBa_K177005	.	terminator	9708	9748	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K177005	.	engineered_region	9708	9748	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177005	.	polyA_site	9735	9748	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177005	.	stop_codon	9741	9741	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177005	.	stem_loop	9715	9734	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177005	.	terminator	9620	9699	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K177005	.	engineered_region	9620	9699	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177005	.	stem_loop	9631	9674	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K177005	.	ribosome_entry_site	1042	1054	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177004
BBa_K177005	.	engineered_region	1042	1054	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177005	.	ribosome_entry_site	1048	1051	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177005	.	ribosome_entry_site	1042	1054	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177005	.	ribosome_entry_site	2222	2234	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177004
BBa_K177005	.	engineered_region	2222	2234	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177005	.	ribosome_entry_site	2228	2231	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177005	.	ribosome_entry_site	2222	2234	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177005	.	CDS	5211	6739	.	+	0	ID=BBa_J61044;Name=BBa_J61044;Parent=BBa_K177004
BBa_K177005	.	CDS	1061	2188	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_K177004
BBa_K177005	.	engineered_tag	2189	2213	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K177005	.	engineered_region	1061	2188	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K177005	.	CDS	1061	2188	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K177005	.	tag	2150	2188	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K177005	.	engineered_region	1	976	.	+	0	ID=BBa_K177000;Name=BBa_K177000
BBa_K177005	.	ribosome_entry_site	46	58	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177000
BBa_K177005	.	engineered_region	46	58	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177005	.	ribosome_entry_site	52	55	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177005	.	ribosome_entry_site	46	58	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177005	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_K177000
BBa_K177005	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K177005	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K177005	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K177005	.	CDS	65	839	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K177000
BBa_K177005	.	tag	776	808	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K177005	.	CDS	68	775	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K177005	.	engineered_tag	815	839	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K177005	.	terminator	848	976	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K177000
BBa_K177005	.	terminator	936	976	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K177005	.	engineered_region	936	976	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177005	.	polyA_site	963	976	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177005	.	stop_codon	969	969	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177005	.	stem_loop	943	962	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177005	.	terminator	848	927	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K177005	.	engineered_region	848	927	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177005	.	stem_loop	859	902	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K177005
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagtcacacaggaaagtactagatgagcacaaaaaag
aaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggct
tatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcatt
aaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaa
atctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttc
aggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagc
cagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgac
ggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagt
ttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatg
caatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaa
aactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataat
ggttgctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatg
ccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtgga
agcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggc
gttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtg
ccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacg
cgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccg
gcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcg
tggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcg
tctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatg
tccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatgg
cgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgatac
cgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggac
cgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaacca
ccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccg
actggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatct
ctactagagtcacacaggaaagtactagatgatggttttccagccgatctctgagtttctgctgatccgtaacgctggt
atgtctatgtacttcaacaaaatcatctctttcaacatcatctctcgtatcgttatctgcatcttcctgatctgcggta
tgttcatggctggtgcttctgaaaaatacgacgctaacgctccgcagcaggttcagccgtactctgtttcttcttctgc
tttcgaaaacctgcacccgaacaacgaaatggaatcttctatcaacccgttctctgcttctgacaccgaacgtaacgct
gctatcatcgaccgtgctaacaaagaacaggaaaccgaagctgttaacaaaatgatctctaccggtgctcgtctggctg
cttctggtcgtgcttctgacgttgctcactctatggttggtgacgctgttaaccaggaaatcaaacagtggctgaaccg
tttcggtaccgctcaggttaacctgaacttcgacaaaaacttctctctgaaagaatcttctctggactggctggctccg
tggtacgactctgcttctttcctgttcttctctcagctgggtatccgtaacaaagactctcgtaacaccctgaacctgg
gtgttggtatccgtaccctggaaaacggttggctgtacggtctgaacaccttctacgacaacgacctgaccggtcacaa
ccaccgtatcggtctgggtgctgaagcttggaccgactacctccaactggctgctaacggttacttccgtctgaacggt
tggcactcttctcgtgacttctctgactacaaagaacgtccggctaccggtggtgacctgcgtgctaacgcttacctgc
cggctctgccgcagctgggtggtaaactgatgtacgaacagtacaccggtgaacgtgttgctctgttcggtaaagacaa
cctccaacgtaacccgtacgctgttaccgctggtatcaactacaccccggttccgctgctgaccgttggtgttgaccag
cgtatgggtaaatcttctaaacacgaaacccagtggaacctccaaatgaactaccgtctgggtgaatctttccagtctc
agctgtctccgtctgctgttgctggtacccgtctgctggctgaatctcgttacaacctggttgaccgtaacaacaacat
cgttctggaataccagaaacagcaggttgttaaactgaccctgtctccggctaccatctctggtctgccgggtcaggtt
taccaggttaacgctcaggttcagggtgcttctgctgttcgtgaaatcgtttggtctgacgctgaactgatcgctgctg
gtggtaccctgaccccgctgtctaccacccagttcaacctggttctgccgccgtacaaacgtaccgctcaggtttctcg
tgttaccgacgacctgaccgctaacttctactctctgtctgctctggctgttgaccaccagggtaaccgttctaactct
ttcaccctgtctgttaccgttcagcagccgcagctgaccctgaccgctgctgttatcggtgacggtgctccggctaacg
gtaaaaccgctatcaccgttgagtttaccgttgctgacttcgaaggtaaaccgctggctggtcaggaagttgttatcac
caccaacaacggtgctctgccgaacaaaatcaccgaaaaaaccgacgctaacggtgttgctcgtatcgctctgaccaac
accaccgacggtgttaccgttgttaccgctgaagttgaaggtcagcgtcagtctgttgacacccacttcgttaaaggta
ccatcgctgctgacaaatctaccctggctgctgttccgacctctatcatcgctgacggtctgatggcttctaccatcac
cctggaactgaaagacacctacggtgacccgcaggctggtgctaacgttgctttcgacaccaccctgggtaacatgggt
gttatcaccgaccacaacgacggtacctactctgctccgctgacctctaccaccctgggtgttgctaccgttaccgtta
aagttgacggtgctgctttctctgttccgtctgttaccgttaacttcaccgctgacccgatcccggacgctggtcgttc
ttctttcaccgtttctaccccggacatcctggctgacggtaccatgtcttctaccctgtctttcgttccggttgacaaa
aacggtcacttcatctctggtatgcagggtctgtctttcacccagaacggtgttccggtttctatctctccgatcaccg
aacagccggactcttacaccgctaccgttgttggtaactctgttggtgacgttaccatcaccccgcaggttgacaccct
gatcctgtctaccctccaaaaaaaaatctctctgttcccggttccgaccctgaccggtatcctggttaacggtcagaac
ttcgctaccgacaaaggtttcccgaaaaccatcttcaaaaacgctaccttccagctccaaatggacaacgacgttgcta
acaacacccagtacgaatggtcttcttctttcaccccgaacgtttctgttaacgaccagggtcaggttaccatcaccta
ccagacctactctgaagttgctgttaccgctaaatctaaaaaattcccgtcttactctgtttcttaccgtttctacccg
aaccgttggatctacgacggtggtcgttctctggtttcttctctggaagcttctcgtcagtgccagggttctgacatgt
ctgctgttctggaatcttctcgtgctaccaacggtacccgtgctccggacggtaccctgtggggtgaatggggttctct
gaccgcttactcttctgactggcagtctggtgaatactgggttaaaaaaacctctaccgacttcgaaaccatgaacatg
gacaccggtgctctccaaccgggtccggcttacctggctttcccgctgtgcgctctgtctatctaataatactagatga
aggatgcatctgcattcaataaagaaaattcaatttcatccatggcaccaccagcatctccgcctgcaagtcctaagac
gccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagta
taccacggagatgcagtgacaaatgtgccgccaagaaaaggttacaaagatggaaatgaatatattgttgtggagaaaa
agaagaaatccatcaatcaaaataatgcagacattcaagttgtgaatgcaatttcgagcctaacctatccaggtgctct
cgtaaaagcgaactcggaattagtagaaaatcaaccagatgttctccctgtaaaacgtgattcattaacactcagcatt
gatttgccaggtatgactaatcaagacaataaaatagttgtaaaaaatgccactaaatcaaacgttaacaacgcagtaa
atacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacga
aatggcttacagtgaatcacaattaattgcgaaatttggtacagcatttaaagctgtaaataatagcttgaatgtaaac
ttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatg
aacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaa
tcctcctgcatatatctcaagtgtggcgtatggccgtcaagtttatttgaaattatcaactaattcccatagtactaaa
gtaaaagctgcttttgatgctgccgtaagcggaaaatctgtctcaggtgatgtagaactaacaaatatcatcaaaaatt
cttccttcaaagccgtaatttacggaggttccgcaaaagatgaagttcaaatcatcgacggcaacctcggagacttacg
cgatattttgaaaaaaggcgctacttttaatcgagaaacaccaggagttcccattgcttatacaacaaacttcctaaaa
gacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaatta
acatcgatcactctggaggatacgttgctcaattcaacatttcttgggatgaagtaaattatgatcctgaaggtaacga
aattgttcaacataaaaactggagcgaaaacaataaaagcaagctagctcatttcacatcgtccatctatttgcctggt
aacgcgagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgacc
ggaacttaccacttgtgaaaaatagaaatatctccatctggggcaccacgctttatccgaaatatagtaataaagtaga
taatccaatcgaataataagaatttactagagctccaggattcaggtcaccaggtcgatgccgcagaagatgccaggga
agctgattactaccttaatgaacaccttccggatatcgctattgtcgatttaggtctgccggatgaagacggcctttcc
ttaatacgccgctggcgcagcagtgatgtttcactgccggttctggtgttaaccgcgcgcgaaggctggcaggataaag
tcgaggttctcagctccggggccgatgactacgtgacgaagccattccacatcgaagaggtaatggcgcgtatgcaggc
gttaatgcgccgtaatagcggtctggcctcccaggtgatcaacatcccgccgttccaggtggatctctcacgccgggaa
ttatccgtcaatgaagaggtcatcaaactcacggcgttcgaatacaccattatggaaacgcttatccgtaacaacggta
aagtggtcagcaaagattcgctgatgcttcagctgtatccggatgcggaactgcgggaaagtcataccattgatgttct
catggggcgtctgcggaaaaaaatacaggcccagtatccgcacgatgtcattaccaccgtacgcggacaaggatatctt
tttgaattgcgctaatgaataaatttgctcgccattttctgccgctgtcgctgcgggttcgttttttgctggcgacagc
cggcgtcgtgctggtgctttctttggcatatggcatagtggcgctggtcggctatagcgtaagttttgataaaaccacc
tttcgtttgctgcgcggcgaaagcaacctgttttataccctcgccaaatgggaaaataataaaatcagcgttgagctgc
ctgaaaatctggacatgcaaagcccgaccatgacgctgatttacgatgaaacgggcaaattattatggacgcagcgcaa
cattccctggctgattaaaagcattcaaccggaatggttaaaaacgaacggcttccatgaaattgaaaccaacgtagac
gccaccagcacgctgttgagcgaagaccattccgcgcaggaaaaactcaaagaagtacgtgaagatgacgatgatgccg
agatgacccactcggtagcggtaaatatttatcctgccacggcgcggatgccgcagttaaccatcgtggtggtcgatac
cattccgatagaactaaaacgctcctatatggtgtggagctggttcgtatacgtgctggccgccaatttactgttagtc
attcctttactgtggatcgccgcctggtggagcttacgccctatcgaggcgctggcgcgggaagtccgcgagcttgaag
atcatcaccgcgaaatgctcaatccggagacgacgcgtgagctgaccagccttgtgcgcaaccttaatcaactgctcaa
aagcgagcgtgaacgttataacaaataccgcacgaccctgaccgacctgacgcacagtttaaaaacgccgctcgcggtt
ttgcagagtacgttacgctctttacgcaacgaaaagatgagcgtcagcaaagctgaaccggtgatgctggaacagatca
gccggatttcccagcagatcggctattatctgcatcgcgccagtatgcgcggtagcggcgtgttgttaagccgcgaact
gcatcccgtcgcgccgttgttagataacctgatttctgcgctaaataaagtttatcagcgtaaaggggtgaatatcagt
atggatatttcaccagaaatcagttttgtcggcgagcaaaacgactttgtcgaagtgatgggcaacgtactggacaacg
cttgtaaatattgtctggagtttgtcgagatttcggctcgccagaccgacgatcatttgcatattttcgtcgaagatga
cggcccaggcattccccacagcaaacgttccctggtgtttgatcgcggtcagcgcgccgataccctacgaccaggacaa
ggcgtggggctggctgtcgcgcgcgagattacggaacaatacgccgggcagatcattgccagcgacagtctgctcggtg
gcgcccgtatggaggtcgtttttggccgacagcatcccacacagaaagaggaataaacatttctgtgcgttcttccacg
catctcgttaagcatccgttataatcggtgtcagatatactagatggtaagcaaaggagaggaattatttacaggagtt
gttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgcta
cctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccct
gggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaa
ggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtg
acaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaata
caactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcac
aacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgc
cggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctgga
gtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata