BBa_K177005
type: ComponentDefinition
persistent identity: BBa K177005
display id: BBa_K177005
version: 1
was generated by: iGEM to SBOL conversion
description: Bistable_1
created: 2009-02-18T12:00:00Z
modified: 2015-05-08T01:11:05Z
owned by: james
owned by: myers
mutable description: Bistable switch for lysteriolysin/invasin expression control
discontinued: false
dominant: false
group u list: _284_
m user id: 0
owner id: 2582
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Michael Lower
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K177000
component: BBa_K177004
sequence annotation: BBa_K177000
sequence annotation: BBa_K177004
sequence: https://synbiohub.org/public/igem/BBa_K177005_sequence/1

BBa_K177000
type: Component
persistent identity: component2305943
display id: component2305943
version: 1
top level: BBa_K177005
definition: BBa_K177000
access: public

BBa_K177004
type: Component
persistent identity: component2305973
display id: component2305973
version: 1
top level: BBa_K177005
definition: BBa_K177004
access: public

BBa_K177000
type: SequenceAnnotation
persistent identity: annotation2305943
display id: annotation2305943
version: 1
top level: BBa_K177005
location: https://synbiohub.org/public/igem/BBa_K177005/annotation2305943/range2305943/1
component: BBa_K177000

https://synbiohub.org/public/igem/BBa_K177005/annotation2305943/range2305943/1
type: Range
persistent identity: range2305943
display id: range2305943
version: 1
top level: BBa_K177005
direction: direction/reverse
start: 1
end: 976
orientation: inline

BBa_K177004
type: SequenceAnnotation
persistent identity: annotation2305973
display id: annotation2305973
version: 1
top level: BBa_K177005
location: https://synbiohub.org/public/igem/BBa_K177005/annotation2305973/range2305973/1
component: BBa_K177004

https://synbiohub.org/public/igem/BBa_K177005/annotation2305973/range2305973/1
type: Range
persistent identity: range2305973
display id: range2305973
version: 1
top level: BBa_K177005
direction: direction/reverse
start: 985
end: 9748
orientation: inline

BBa_K177000
type: ComponentDefinition
persistent identity: BBa K177000
display id: BBa_K177000
version: 1
was generated by: iGEM to SBOL conversion
description: Bistable_I part L
created: 2009-02-18T12:00:00Z
modified: 2015-05-08T01:11:05Z
owned by: james
owned by: myers
mutable description: left part of the bistable alpha construct. Contains cI gene under Plac
discontinued: false
dominant: false
group u list: _284_
m user id: 0
owner id: 2582
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Michael Lower
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0015
component: BBa_C0051
component: BBa_B0032
component: BBa_I14032
sequence annotation: BBa_I14032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K177000_sequence/1

BBa_B0015
type: Component
persistent identity: component2255031
display id: component2255031
version: 1
top level: BBa_K177000
definition: BBa_B0015
access: public

BBa_C0051
type: Component
persistent identity: component2255024
display id: component2255024
version: 1
top level: BBa_K177000
definition: cI lam
access: public

BBa_B0032
type: Component
persistent identity: component2255019
display id: component2255019
version: 1
top level: BBa_K177000
definition: BBa_B0032
access: public

BBa_I14032
type: Component
persistent identity: component2255017
display id: component2255017
version: 1
top level: BBa_K177000
definition: P(Lac) IQ
access: public

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2255017
display id: annotation2255017
version: 1
top level: BBa_K177000
location: https://synbiohub.org/public/igem/BBa_K177000/annotation2255017/range2255017/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K177000/annotation2255017/range2255017/1
type: Range
persistent identity: range2255017
display id: range2255017
version: 1
top level: BBa_K177000
direction: direction/reverse
start: 1
end: 37
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2255019
display id: annotation2255019
version: 1
top level: BBa_K177000
location: https://synbiohub.org/public/igem/BBa_K177000/annotation2255019/range2255019/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K177000/annotation2255019/range2255019/1
type: Range
persistent identity: range2255019
display id: range2255019
version: 1
top level: BBa_K177000
direction: direction/forward
start: 46
end: 58
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2255024
display id: annotation2255024
version: 1
top level: BBa_K177000
location: https://synbiohub.org/public/igem/BBa_K177000/annotation2255024/range2255024/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K177000/annotation2255024/range2255024/1
type: Range
persistent identity: range2255024
display id: range2255024
version: 1
top level: BBa_K177000
direction: direction/reverse
start: 65
end: 839
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2255031
display id: annotation2255031
version: 1
top level: BBa_K177000
location: https://synbiohub.org/public/igem/BBa_K177000/annotation2255031/range2255031/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K177000/annotation2255031/range2255031/1
type: Range
persistent identity: range2255031
display id: range2255031
version: 1
top level: BBa_K177000
direction: direction/reverse
start: 848
end: 976
orientation: inline

BBa_K177004
type: ComponentDefinition
persistent identity: BBa K177004
display id: BBa_K177004
version: 1
was generated by: iGEM to SBOL conversion
description: bistable_1_R
created: 2009-02-18T12:00:00Z
modified: 2015-05-08T01:11:05Z
owned by: james
owned by: myers
mutable description: right portion of bistable 1 switch
discontinued: false
dominant: false
group u list: _284_
m user id: 0
owner id: 2582
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Michael Lower
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0015
component: BBa_J61044
component: BBa_I10001
component: BBa_Z0301
component: BBa_K177007
component: BBa_B0032
component: BBa_R0051
component: BBa_C0012
sequence annotation: BBa_R0051
sequence annotation: BBa_B0032
sequence annotation: BBa_C0012
sequence annotation: BBa_I10001
sequence annotation: BBa_J61044
sequence annotation: BBa_K177007
sequence annotation: BBa_Z0301
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K177004_sequence/1

BBa_B0015
type: Component
persistent identity: component2283191
display id: component2283191
version: 1
top level: BBa_K177004
definition: BBa_B0015
access: public

BBa_J61044
type: Component
persistent identity: component2283179
display id: component2283179
version: 1
top level: BBa_K177004
definition: BBa_J61044
access: public

BBa_I10001
type: Component
persistent identity: component2283178
display id: component2283178
version: 1
top level: BBa_K177004
definition: BBa_I10001
access: public

BBa_Z0301
type: Component
persistent identity: component2283184
display id: component2283184
version: 1
top level: BBa_K177004
definition: BBa_Z0301
access: public

BBa_K177007
type: Component
persistent identity: component2283182
display id: component2283182
version: 1
top level: BBa_K177004
definition: BBa_K177007
access: public

BBa_B0032
type: Component
persistent identity: component2283169
display id: component2283169
version: 1
top level: BBa_K177004
definition: BBa_B0032
access: public

BBa_R0051
type: Component
persistent identity: component2283164
display id: component2283164
version: 1
top level: BBa_K177004
definition: cI lam
access: public

BBa_B0032
type: Component
persistent identity: component2283176
display id: component2283176
version: 1
top level: BBa_K177004
definition: BBa_B0032
access: public

BBa_C0012
type: Component
persistent identity: component2283172
display id: component2283172
version: 1
top level: BBa_K177004
definition: lacI
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2283164
display id: annotation2283164
version: 1
top level: BBa_K177004
location: https://synbiohub.org/public/igem/BBa_K177004/annotation2283164/range2283164/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K177004/annotation2283164/range2283164/1
type: Range
persistent identity: range2283164
display id: range2283164
version: 1
top level: BBa_K177004
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2283169
display id: annotation2283169
version: 1
top level: BBa_K177004
location: https://synbiohub.org/public/igem/BBa_K177004/annotation2283169/range2283169/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K177004/annotation2283169/range2283169/1
type: Range
persistent identity: range2283169
display id: range2283169
version: 1
top level: BBa_K177004
direction: direction/forward
start: 58
end: 70
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2283172
display id: annotation2283172
version: 1
top level: BBa_K177004
location: https://synbiohub.org/public/igem/BBa_K177004/annotation2283172/range2283172/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K177004/annotation2283172/range2283172/1
type: Range
persistent identity: range2283172
display id: range2283172
version: 1
top level: BBa_K177004
direction: direction/forward
start: 77
end: 1204
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2283176
display id: annotation2283176
version: 1
top level: BBa_K177004
location: https://synbiohub.org/public/igem/BBa_K177004/annotation2283176/range2283176/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K177004/annotation2283176/range2283176/1
type: Range
persistent identity: range2283176
display id: range2283176
version: 1
top level: BBa_K177004
direction: direction/forward
start: 1238
end: 1250
orientation: inline

BBa_I10001
type: SequenceAnnotation
persistent identity: annotation2283178
display id: annotation2283178
version: 1
top level: BBa_K177004
location: https://synbiohub.org/public/igem/BBa_K177004/annotation2283178/range2283178/1
component: BBa_I10001

https://synbiohub.org/public/igem/BBa_K177004/annotation2283178/range2283178/1
type: Range
persistent identity: range2283178
display id: range2283178
version: 1
top level: BBa_K177004
direction: direction/reverse
start: 1257
end: 4220
orientation: inline

BBa_J61044
type: SequenceAnnotation
persistent identity: annotation2283179
display id: annotation2283179
version: 1
top level: BBa_K177004
location: https://synbiohub.org/public/igem/BBa_K177004/annotation2283179/range2283179/1
component: BBa_J61044

https://synbiohub.org/public/igem/BBa_K177004/annotation2283179/range2283179/1
type: Range
persistent identity: range2283179
display id: range2283179
version: 1
top level: BBa_K177004
direction: direction/reverse
start: 4227
end: 5755
orientation: inline

BBa_K177007
type: SequenceAnnotation
persistent identity: annotation2283182
display id: annotation2283182
version: 1
top level: BBa_K177004
location: https://synbiohub.org/public/igem/BBa_K177004/annotation2283182/range2283182/1
component: BBa_K177007

https://synbiohub.org/public/igem/BBa_K177004/annotation2283182/range2283182/1
type: Range
persistent identity: range2283182
display id: range2283182
version: 1
top level: BBa_K177004
direction: direction/reverse
start: 5764
end: 7901
orientation: inline

BBa_Z0301
type: SequenceAnnotation
persistent identity: annotation2283184
display id: annotation2283184
version: 1
top level: BBa_K177004
location: https://synbiohub.org/public/igem/BBa_K177004/annotation2283184/range2283184/1
component: BBa_Z0301

https://synbiohub.org/public/igem/BBa_K177004/annotation2283184/range2283184/1
type: Range
persistent identity: range2283184
display id: range2283184
version: 1
top level: BBa_K177004
direction: direction/reverse
start: 7908
end: 8627
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2283191
display id: annotation2283191
version: 1
top level: BBa_K177004
location: https://synbiohub.org/public/igem/BBa_K177004/annotation2283191/range2283191/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K177004/annotation2283191/range2283191/1
type: Range
persistent identity: range2283191
display id: range2283191
version: 1
top level: BBa_K177004
direction: direction/reverse
start: 8636
end: 8764
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: iGEM to SBOL conversion
description: promoter P(Lac) IQ
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, High Transcription
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 26
end: 31
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I10001
type: ComponentDefinition
persistent identity: BBa I10001
display id: BBa_I10001
version: 1
was generated by: iGEM to SBOL conversion
description: Invasin
created: 2006-04-03T11:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: Invasin is a bacterial membrane protein (from Y.pseudotuberculosis) that binds tightly to beta-1-integrins found on the surface of many mamallian cells; interaction between invasin and beta-1-integrins induces the physical uptake of bacteria by mamallian cells via a Rac-1 mediated response (Wong & Isberg, 2005; PMID: 15694850)
discontinued: false
dominant: false
experience: experience/Works
group u list: _89_45_47_11_12_21_8_48_46_1_10_43_
m user id: 0
owner id: 52
owning group id: 89
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Chris Anderson (Voigt Lab)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I10001_sequence/1

BBa_J61044
type: ComponentDefinition
persistent identity: BBa J61044
display id: BBa_J61044
version: 1
was generated by: iGEM to SBOL conversion
description: [LLO] Listeriolysin open reading frame
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: bob
star: false
creator: John Anderson
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J61044_sequence/1

BBa_K177007
type: ComponentDefinition
persistent identity: BBa K177007
display id: BBa_K177007
version: 1
was generated by: iGEM to SBOL conversion
description: PhoP/PhoQ locus from salmonella typhimurium
created: 2009-02-18T12:00:00Z
modified: 2015-05-08T01:11:05Z
owned by: james
owned by: myers
mutable description: pH sensing locus from S. typh
discontinued: false
dominant: false
group u list: _284_
m user id: 0
owner id: 2582
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Michael Lower
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: PhoP
sequence annotation: PhoQ
sequence: https://synbiohub.org/public/igem/BBa_K177007_sequence/1

PhoP
type: SequenceAnnotation
persistent identity: annotation2000913
display id: annotation2000913
version: 1
top level: BBa_K177007
location: https://synbiohub.org/public/igem/BBa_K177007/annotation2000913/range2000913/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K177007/annotation2000913/range2000913/1
type: Range
persistent identity: range2000913
display id: range2000913
version: 1
top level: BBa_K177007
direction: direction/forward
start: 1
end: 675
orientation: inline

PhoQ
type: SequenceAnnotation
persistent identity: annotation2000914
display id: annotation2000914
version: 1
top level: BBa_K177007
location: https://synbiohub.org/public/igem/BBa_K177007/annotation2000914/range2000914/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K177007/annotation2000914/range2000914/1
type: Range
persistent identity: range2000914
display id: range2000914
version: 1
top level: BBa_K177007
direction: direction/forward
start: 675
end: 2138
orientation: inline

BBa_Z0301
type: ComponentDefinition
persistent identity: BBa Z0301
display id: BBa_Z0301
version: 1
was generated by: iGEM to SBOL conversion
description: Venus YFP - E. coli codon optimized
created: 2006-06-20T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: Venus Sequence obtained from Prof. Miyawaki. Codon optimized from Gene Design by Boeke Lab.
owned by: james
owned by: myers
mutable description: Brighter Variant than standard EYFP.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 10
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None really
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: gtc ...gtt -> gta ... gtt codon shuffle
sequence: https://synbiohub.org/public/igem/BBa_Z0301_sequence/1

gtc ...gtt -> gta ... gtt codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881736
display id: annotation1881736
version: 1
top level: BBa_Z0301
location: https://synbiohub.org/public/igem/BBa_Z0301/annotation1881736/range1881736/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0301/annotation1881736/range1881736/1
type: Range
persistent identity: range1881736
display id: range1881736
version: 1
top level: BBa_Z0301
direction: direction/forward
start: 4
end: 39
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K177005_sequence/1
type: Sequence
persistent identity: BBa K177005 sequence
display id: BBa_K177005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagtcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K177000_sequence/1
type: Sequence
persistent identity: BBa K177000 sequence
display id: BBa_K177000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagtcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K177004_sequence/1
type: Sequence
persistent identity: BBa K177004 sequence
display id: BBa_K177004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacaggaaagtactagatgatggttttccagccgatctctgagtttctgctgatccgtaacgctggtatgtctatgtacttcaacaaaatcatctctttcaacatcatctctcgtatcgttatctgcatcttcctgatctgcggtatgttcatggctggtgcttctgaaaaatacgacgctaacgctccgcagcaggttcagccgtactctgtttcttcttctgctttcgaaaacctgcacccgaacaacgaaatggaatcttctatcaacccgttctctgcttctgacaccgaacgtaacgctgctatcatcgaccgtgctaacaaagaacaggaaaccgaagctgttaacaaaatgatctctaccggtgctcgtctggctgcttctggtcgtgcttctgacgttgctcactctatggttggtgacgctgttaaccaggaaatcaaacagtggctgaaccgtttcggtaccgctcaggttaacctgaacttcgacaaaaacttctctctgaaagaatcttctctggactggctggctccgtggtacgactctgcttctttcctgttcttctctcagctgggtatccgtaacaaagactctcgtaacaccctgaacctgggtgttggtatccgtaccctggaaaacggttggctgtacggtctgaacaccttctacgacaacgacctgaccggtcacaaccaccgtatcggtctgggtgctgaagcttggaccgactacctccaactggctgctaacggt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14032_sequence/1
type: Sequence
persistent identity: BBa I14032 sequence
display id: BBa_I14032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I10001_sequence/1
type: Sequence
persistent identity: BBa I10001 sequence
display id: BBa_I10001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatggttttccagccgatctctgagtttctgctgatccgtaacgctggtatgtctatgtacttcaacaaaatcatctctttcaacatcatctctcgtatcgttatctgcatcttcctgatctgcggtatgttcatggctggtgcttctgaaaaatacgacgctaacgctccgcagcaggttcagccgtactctgtttcttcttctgctttcgaaaacctgcacccgaacaacgaaatggaatcttctatcaacccgttctctgcttctgacaccgaacgtaacgctgctatcatcgaccgtgctaacaaagaacaggaaaccgaagctgttaacaaaatgatctctaccggtgctcgtctggctgcttctggtcgtgcttctgacgttgctcactctatggttggtgacgctgttaaccaggaaatcaaacagtggctgaaccgtttcggtaccgctcaggttaacctgaacttcgacaaaaacttctctctgaaagaatcttctctggactggctggctccgtggtacgactctgcttctttcctgttcttctctcagctgggtatccgtaacaaagactctcgtaacaccctgaacctgggtgttggtatccgtaccctggaaaacggttggctgtacggtctgaacaccttctacgacaacgacctgaccggtcacaaccaccgtatcggtctgggtgctgaagcttggaccgactacctccaactggctgctaacggttacttccgtctgaacggttggcactcttctcgtgacttctctgactacaaagaacgtccggctaccggtggtgacctgcgtgctaacgcttacctgccggctctgccgcagctgggtggtaaactgatgtacgaacagtacaccggtgaacgtgttgctctgttcggtaaagacaacctccaacgtaacccgtacgctgttaccgctggtatcaactacaccccggttccgctgctgaccgttggtgttgaccagcgtatgggtaaatcttctaaacacgaaacccagtggaacctccaaatgaactaccgtctgggtgaatctttccagtctcagctgtctccgtctgctgttgctggtacccgtctgctggctgaatctcgttacaacctggttgaccgtaacaacaacatcgttctggaataccagaaacagcaggttgttaaactgaccctgtctccggctaccatctctggtctgccgggtcaggtttaccaggttaacgctcaggttcagggtgcttctgctgttcgtgaaatcgtttggtctgacgctgaactgatcgctgctggtggtaccctgaccccgctgtctaccacccagttcaacctggttctgccgccgtacaaacgtaccgctcaggtttctcgtgttaccgacgacctgaccgctaacttctactctctgtctgctctggctgttgaccaccagggtaaccgttctaactctttcaccctgtctgttaccgttcagcagccgcagctgaccctgaccgctgctgttatcggtgacggtgctccggctaacggtaaaaccgctatcaccgttgagtttaccgttgctgacttcgaaggtaaaccgctggctggtcaggaagttgttatcaccaccaacaacggtgctctgccgaacaaaatcaccgaaaaaaccgacgctaacggtgttgctcgtatcgctctgaccaacaccaccgacggtgttaccgttgttaccgctgaagttgaaggtcagcgtcagtctgttgacacccacttcgttaaaggtaccatcgctgctgacaaatctaccctggctgctgttccgacctctatcatcgctgacggtctgatggcttctaccatcaccctggaactgaaagacacctacggtgacccgcaggctggtgctaacgttgctttcgacaccaccctgggtaacatgggtgttatcaccgaccacaacgacggtacctactctgctccgctgacctctaccac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61044_sequence/1
type: Sequence
persistent identity: BBa J61044 sequence
display id: BBa_J61044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaggatgcatctgcattcaataaagaaaattcaatttcatccatggcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttacaaagatggaaatgaatatattgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagacattcaagttgtgaatgcaatttcgagcctaacctatccaggtgctctcgtaaaagcgaactcggaattagtagaaaatcaaccagatgttctccctgtaaaacgtgattcattaacactcagcattgatttgccaggtatgactaatcaagacaataaaatagttgtaaaaaatgccactaaatcaaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcgaaatttggtacagcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcgtatggccgtcaagtttatttgaaattatcaactaattcccatagtactaaagtaaaagctgcttttgatgctgccgtaagcggaaaatctgtctcaggtgatgtagaactaacaaatatcatcaaaaattcttccttcaaagccgtaatttacggaggttccgcaaaagatgaagttcaaatcatcgacggcaacctcggagacttacgcgatattttgaaaaaaggcgctacttttaatcgagaaacaccaggagttcccattgcttatacaacaaacttcctaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaattaacatcgatcactctggaggatacgttgctcaattcaacatttcttgggatgaagtaaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagcaagctagctcatttcacatcgtccatctatttgcctggtaacgcgagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacttaccacttgtgaaaaatagaaatatctccatctggggcaccacgctttatccgaaatatagtaataaagtagataatccaatcgaataataagaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K177007_sequence/1
type: Sequence
persistent identity: BBa K177007 sequence
display id: BBa_K177007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctccaggattcaggtcaccaggtcgatgccgcagaagatgccagggaagctgattactaccttaatgaacaccttccggatatcgctattgtcgatttaggtctgccggatgaagacggcctttccttaatacgccgctggcgcagcagtgatgtttcactgccggttctggtgttaaccgcgcgcgaaggctggcaggataaagtcgaggttctcagctccggggccgatgactacgtgacgaagccattccacatcgaagaggtaatggcgcgtatgcaggcgttaatgcgccgtaatagcggtctggcctcccaggtgatcaacatcccgccgttccaggtggatctctcacgccgggaattatccgtcaatgaagaggtcatcaaactcacggcgttcgaatacaccattatggaaacgcttatccgtaacaacggtaaagtggtcagcaaagattcgctgatgcttcagctgtatccggatgcggaactgcgggaaagtcataccattgatgttctcatggggcgtctgcggaaaaaaatacaggcccagtatccgcacgatgtcattaccaccgtacgcggacaaggatatctttttgaattgcgctaatgaataaatttgctcgccattttctgccgctgtcgctgcgggttcgttttttgctggcgacagccggcgtcgtgctggtgctttctttggcatatggcatagtggcgctggtcggctatagcgtaagttttgataaaaccacctttcgtttgctgcgcggcgaaagcaacctgttttataccctcgccaaatgggaaaataataaaatcagcgttgagctgcctgaaaatctggacatgcaaagcccgaccatgacgctgatttacgatgaaacgggcaaattattatggacgcagcgcaacattccctggctgattaaaagcattcaaccggaatggttaaaaacgaacggcttccatgaaattgaaaccaacgtagacgccaccagcacgctgttgagcgaagaccattccgcgcaggaaaaactcaaagaagtacgtgaagatgacgatgatgccgagatgacccactcggtagcggtaaatatttatcctgccacggcgcggatgccgcagttaaccatcgtggtggtcgataccattccgatagaactaaaacgctcctatatggtgtggagctggttcgtatacgtgctggccgccaatttactgttagtcattcctttactgtggatcgccgcctggtggagcttacgccctatcgaggcgctggcgcgggaagtccgcgagcttgaagatcatcaccgcgaaatgctcaatccggagacgacgcgtgagctgaccagccttgtgcgcaaccttaatcaactgctcaaaagcgagcgtgaacgttataacaaataccgcacgaccctgaccgacctgacgcacagtttaaaaacgccgctcgcggttttgcagagtacgttacgctctttacgcaacgaaaagatgagcgtcagcaaagctgaaccggtgatgctggaacagatcagccggatttcccagcagatcggctattatctgcatcgcgccagtatgcgcggtagcggcgtgttgttaagccgcgaactgcatcccgtcgcgccgttgttagataacctgatttctgcgctaaataaagtttatcagcgtaaaggggtgaatatcagtatggatatttcaccagaaatcagttttgtcggcgagcaaaacgactttgtcgaagtgatgggcaacgtactggacaacgcttgtaaatattgtctggagtttgtcgagatttcggctcgccagaccgacgatcatttgcatattttcgtcgaagatgacggcccaggcattccccacagcaaacgttccctggtgtttgatcgcggtcagcgcgccgataccctacgaccaggacaaggcgtggggctggctgtcgcgcgcgagattacggaacaatacgccgggcagatcatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0301_sequence/1
type: Sequence
persistent identity: BBa Z0301 sequence
display id: BBa_Z0301_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z