LOCUS BBa_K177006 2922 bp DNA linear 22-Dec-2024 DEFINITION Bistable_2 ACCESSION BBa_K177006 VERSION BBa_K177006.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 CDS 82..957 /label=araC misc_feature 958..990 /label=LVA misc_feature 997..1021 /label=Help:Barcodes CDS 82..1021 /label=BBa_C0080 stem_loop 1039..1054 /label=stem loop polyA_site 1055..1063 /label=Poly A tail polyA_site 1030..1038 /label=PolyA tail misc_feature 1030..1063 /label=B1001 terminator 1030..1063 /label=BBa_B1001 stem_loop 1081..1096 /label=stem loop polyA_site 1097..1105 /label=Poly A tail polyA_site 1072..1080 /label=PolyA tail misc_feature 1072..1105 /label=B1001 terminator 1072..1105 /label=BBa_B1001 misc_feature 1..1105 /label=BBa_K177001 misc_feature 1117..1142 /label=c-amp2 misc_feature 1119..1157 /label=araO1 misc_feature 1156..1185 /label=c-amp1 misc_feature 1186..1214 /label=ara1 and ara2 promoter 1226..1231 /label=-35 promoter 1249..1254 /label=-10 promoter 1114..1262 /label=BBa_R0080 RBS 1271..1283 /label=RBS-3\Weak RBS 1277..1280 /label=RBS misc_feature 1271..1283 /label=BBa_B0032 RBS 1271..1283 /label=BBa_B0032 CDS 1293..1909 /label=tetR misc_feature 1910..1943 /label=SsrA misc_feature 1950..1974 /label=Help:Barcodes CDS 1290..1974 /label=BBa_C0040 misc_feature 1983..1999 /label=OR1 promoter 1983..1999 /label=BBa_K177002 RBS 2008..2020 /label=RBS-3\Weak RBS 2014..2017 /label=RBS misc_feature 2008..2020 /label=BBa_B0032 RBS 2008..2020 /label=BBa_B0032 misc_feature 2027..2029 /label=start misc_feature 2780..2782 /label=stop misc_feature 2783..2785 /label=stop misc_feature 2743..2779 /label=LVA tag CDS 2030..2742 /label=cds misc_feature 2027..2785 /label=BBa_K145015 stem_loop 2805..2848 /label=stem_loop misc_feature 2794..2873 /label=BBa_B0010 terminator 2794..2873 /label=BBa_B0010 stem_loop 2889..2908 /label=T7 TE misc_feature 2915..2915 /label=stop polyA_site 2909..2922 /label=polya misc_feature 2882..2922 /label=BBa_B0012 terminator 2882..2922 /label=BBa_B0012 terminator 2794..2922 /label=BBa_B0015 misc_feature 1114..2922 /label=BBa_K177003 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaagtacta gatggctgaa gcgcaaaatg atcccctgct gccgggatac 121 tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaatgg ttatctcgat 181 ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt 241 cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga tattttgctg 301 ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac 361 cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata 421 tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg 481 tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata 541 aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca 601 ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc 661 aattttgata tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg tctgtcacat 721 cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca acgcatcagc 781 caggcgaagc tgcttttgag cactacccgg atgcctatcg ccaccgtcgg tcgcaatgtt 841 ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg ggccagcccg 901 agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcagct 961 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 1021 ctactagaga aaaaaaaacc ccgcttcggc ggggtttttt ttttactaga gaaaaaaaaa 1081 ccccgcttcg gcggggtttt ttttttacta gaggcgtaac aaaagtgtct ataatcacgg 1141 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt 1201 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc 1261 attactagag tcacacagga aagtactaga tgtccagatt agataaaagt aaagtgatta 1321 acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg 1381 cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt 1441 tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag 1501 aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac 1561 taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg 1621 aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat 1681 tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc 1741 atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac 1801 gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg 1861 aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg 1921 acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag 1981 agtacctctg gcggtgatat actagagtca cacaggaaag tactagatgc gtaaaggaga 2041 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca 2101 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa 2161 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg 2221 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa 2281 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa 2341 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt 2401 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta 2461 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt 2521 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa 2581 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc 2641 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac 2701 agctgctggg attacacatg gcatggatga actatacaaa aggcctgctg caaacgacga 2761 aaactacgct ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc 2821 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 2881 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //