##gff-version 3
##sequence-region BBa_K177006 1 2922
BBa_K177006	.	engineered_region	1	1105	.	+	0	ID=BBa_K177001;Name=BBa_K177001
BBa_K177006	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177001
BBa_K177006	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177006	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177006	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177006	.	CDS	82	1021	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_K177001
BBa_K177006	.	CDS	82	957	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_K177006	.	engineered_tag	997	1021	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_K177006	.	tag	958	990	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_K177006	.	terminator	1072	1105	.	+	0	ID=BBa_B1001;Name=BBa_B1001;Parent=BBa_K177001
BBa_K177006	.	engineered_region	1072	1105	.	+	0	ID=annotation1898382;Name=B1001;Parent=BBa_B1001
BBa_K177006	.	polyA_site	1072	1080	.	+	0	ID=annotation1898381;Name=PolyA tail;Parent=BBa_B1001
BBa_K177006	.	polyA_site	1097	1105	.	+	0	ID=annotation1898380;Name=Poly A tail;Parent=BBa_B1001
BBa_K177006	.	stem_loop	1081	1096	.	+	0	ID=annotation1898379;Name=stem loop;Parent=BBa_B1001
BBa_K177006	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K177001
BBa_K177006	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K177006	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K177006	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K177006	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K177006	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K177006	.	terminator	1030	1063	.	+	0	ID=BBa_B1001;Name=BBa_B1001;Parent=BBa_K177001
BBa_K177006	.	engineered_region	1030	1063	.	+	0	ID=annotation1898382;Name=B1001;Parent=BBa_B1001
BBa_K177006	.	polyA_site	1030	1038	.	+	0	ID=annotation1898381;Name=PolyA tail;Parent=BBa_B1001
BBa_K177006	.	polyA_site	1055	1063	.	+	0	ID=annotation1898380;Name=Poly A tail;Parent=BBa_B1001
BBa_K177006	.	stem_loop	1039	1054	.	+	0	ID=annotation1898379;Name=stem loop;Parent=BBa_B1001
BBa_K177006	.	engineered_region	1114	2922	.	+	0	ID=BBa_K177003;Name=BBa_K177003
BBa_K177006	.	terminator	2794	2922	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K177003
BBa_K177006	.	terminator	2882	2922	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K177006	.	engineered_region	2882	2922	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177006	.	stem_loop	2889	2908	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177006	.	stop_codon	2915	2915	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177006	.	polyA_site	2909	2922	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177006	.	terminator	2794	2873	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K177006	.	engineered_region	2794	2873	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177006	.	stem_loop	2805	2848	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K177006	.	CDS	1290	1974	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K177003
BBa_K177006	.	engineered_tag	1950	1974	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K177006	.	CDS	1293	1909	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K177006	.	tag	1910	1943	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K177006	.	ribosome_entry_site	2008	2020	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177003
BBa_K177006	.	ribosome_entry_site	2008	2020	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177006	.	ribosome_entry_site	2014	2017	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177006	.	engineered_region	2008	2020	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177006	.	engineered_region	2027	2785	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K177003
BBa_K177006	.	CDS	2030	2742	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K177006	.	stop_codon	2783	2785	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K177006	.	stop_codon	2780	2782	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K177006	.	start_codon	2027	2029	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K177006	.	tag	2743	2779	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K177006	.	ribosome_entry_site	1271	1283	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177003
BBa_K177006	.	ribosome_entry_site	1271	1283	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177006	.	ribosome_entry_site	1277	1280	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177006	.	engineered_region	1271	1283	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177006	.	promoter	1983	1999	.	+	0	ID=BBa_K177002;Name=BBa_K177002;Parent=BBa_K177003
BBa_K177006	.	operator	1983	1999	.	+	0	ID=annotation2000761;Name=OR1;Parent=BBa_K177002
BBa_K177006	.	promoter	1114	1262	.	+	0	ID=BBa_R0080;Name=BBa_R0080;Parent=BBa_K177003
BBa_K177006	.	non_covalent_binding_site	1117	1142	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_K177006	.	operator	1119	1157	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_K177006	.	non_covalent_binding_site	1156	1185	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_K177006	.	promoter	1249	1254	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_K177006	.	non_covalent_binding_site	1186	1214	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_K177006	.	promoter	1226	1231	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
>BBa_K177006
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgat
tgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgc
ggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagaga
ttcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggca
tgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagc
gacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagc
aattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtca
gtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgt
ctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagc
tgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcg
agtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtc
aagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agaaaaaaaaaccccgcttcggcggggttttttttttactagagaaaaaaaaaccccgcttcggcggggtttttttttt
actagaggcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgcta
tgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatta
ctagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaat
gaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatg
taaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttaga
aggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagca
aaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaag
gtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaaga
gcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaatta
tttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaat
gtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcact
actagagtacctctggcggtgatatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactg
gagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtga
tgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcact
actttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgc
ccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttga
aggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattg
gaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatta
gacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcct
tttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcctt
cttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaact
acgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata