LOCUS       BBa_K177008             3097 bp    DNA     linear       01-Jun-2024
DEFINITION  Phagosome localisation device (PhoQ based)
ACCESSION   BBa_K177008
VERSION     BBa_K177008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..531
                     /label=BBa_I716028
     RBS             540..552
                     /label=RBS-3\Weak
     RBS             546..549
                     /label=RBS
     misc_feature    540..552
                     /label=BBa_B0032
     RBS             540..552
                     /label=BBa_B0032
     CDS             562..1269
                     /label=cI lambda
     misc_feature    1270..1302
                     /label=LVA
     misc_feature    1309..1333
                     /label=Help:Barcodes
     CDS             559..1333
                     /label=BBa_C0051
     CDS             1340..1540
                     /label=cro
     CDS             1340..1540
                     /label=BBa_K177009
     CDS             1550..2166
                     /label=tetR
     misc_feature    2167..2200
                     /label=SsrA
     misc_feature    2207..2231
                     /label=Help:Barcodes
     CDS             1547..2231
                     /label=BBa_C0040
     CDS             2238..2945
                     /label=mCherry
     misc_feature    2589..2589
                     /label=C->T (removing PstI site)
     misc_feature    2946..2954
                     /label=LVA
     misc_feature    2238..2960
                     /label=BBa_J06505
     stem_loop       2980..3023
                     /label=stem_loop
     misc_feature    2969..3048
                     /label=BBa_B0010
     terminator      2969..3048
                     /label=BBa_B0010
     stem_loop       3064..3083
                     /label=T7 TE
     misc_feature    3090..3090
                     /label=stop
     polyA_site      3084..3097
                     /label=polya
     misc_feature    3057..3097
                     /label=BBa_B0012
     terminator      3057..3097
                     /label=BBa_B0012
     terminator      2969..3097
                     /label=BBa_B0015
ORIGIN
        1 tgagagtaag aacctgtcgg aatatcaaac agacaggttc tttatttagc atgagaaaaa
       61 taaagttgaa ggtggcgtta tattaaacgc gcttgctata agagtatttt actcaggagt
      121 gagaatctgg ttatttattg cccttaacca ttatcgacca cgatattgct tttgcgtaac
      181 agcgggcaat ctgttatccc caaaaaacca cttttagtgt gcaagtattg taccgtgctg
      241 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
      301 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
      361 actgtgcgaa tgatgaatcg cattacaacc tcttctcttt ttatgttcgc ttaatcgtag
      421 cggcaatatg cgctgaagca agcgactcat tccgaaaaag cacgaatatc gacatagtta
      481 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaaa ctactagagt
      541 cacacaggaa agtactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
      601 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      661 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      721 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      781 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      841 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      901 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      961 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
     1021 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
     1081 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
     1141 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
     1201 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
     1261 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc
     1321 tagtgtagat cgctactaga tggaacaacg cataaccctg aaagattatg caatgcgctt
     1381 tgggcaaacc aagacagcta aagatctcgg cgtatatcaa agcgcgatca acaaggccat
     1441 tcatgcaggc cgaaagattt ttttaactat aaacgctgat ggaagcgttt atgcggaaga
     1501 ggtaaagccc ttcccgagta acaaaaaaac aacagcataa tactagatgt ccagattaga
     1561 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
     1621 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
     1681 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
     1741 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
     1801 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
     1861 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
     1921 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
     1981 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
     2041 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
     2101 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
     2161 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
     2221 gtgtagatca ctactagatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     2281 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     2341 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     2401 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     2461 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     2521 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     2581 actcctcctt gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     2641 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     2701 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     2761 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     2821 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     2881 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     2941 acaagttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     3001 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3061 actggctcac cttcgggtgg gcctttctgc gtttata
//