##gff-version 3
##sequence-region BBa_K177008 1 3097
BBa_K177008	.	terminator	2969	3097	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K177008	.	terminator	2969	3048	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K177008	.	stem_loop	2980	3023	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K177008	.	engineered_region	2969	3048	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177008	.	terminator	3057	3097	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K177008	.	stop_codon	3090	3090	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177008	.	stem_loop	3064	3083	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177008	.	engineered_region	3057	3097	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177008	.	polyA_site	3084	3097	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177008	.	engineered_region	2238	2960	.	+	0	ID=BBa_J06505;Name=BBa_J06505
BBa_K177008	.	CDS	2238	2945	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_K177008	.	sequence_alteration	2589	2589	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_K177008	.	tag	2946	2954	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_K177008	.	CDS	1547	2231	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K177008	.	engineered_tag	2207	2231	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K177008	.	CDS	1550	2166	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K177008	.	tag	2167	2200	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K177008	.	ribosome_entry_site	540	552	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K177008	.	engineered_region	540	552	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177008	.	ribosome_entry_site	540	552	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177008	.	ribosome_entry_site	546	549	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177008	.	sequence_feature	1	531	.	+	0	ID=BBa_I716028;Name=BBa_I716028
BBa_K177008	.	CDS	559	1333	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K177008	.	tag	1270	1302	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K177008	.	CDS	562	1269	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K177008	.	engineered_tag	1309	1333	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K177008	.	CDS	1340	1540	.	+	0	ID=BBa_K177009;Name=BBa_K177009
BBa_K177008	.	CDS	1340	1540	.	+	0	ID=annotation2053853;Name=cro;Parent=BBa_K177009
>BBa_K177008
tgagagtaagaacctgtcggaatatcaaacagacaggttctttatttagcatgagaaaaataaagttgaaggtggcgtt
atattaaacgcgcttgctataagagtattttactcaggagtgagaatctggttatttattgcccttaaccattatcgac
cacgatattgcttttgcgtaacagcgggcaatctgttatccccaaaaaaccacttttagtgtgcaagtattgtaccgtg
ctggtgcctctggcagtcagataggtacattgcaaacctaatcctgcggcattctctttgcttccaatcaaaacgccat
atccgctgagtaataatcctatccataccagtgctatcagcataactgtgcgaatgatgaatcgcattacaacctcttc
tctttttatgttcgcttaatcgtagcggcaatatgcgctgaagcaagcgactcattccgaaaaagcacgaatatcgaca
tagttaggcgctgtttaactaacgcatgctagtttaatgacataaggtaggtgaaactactagagtcacacaggaaagt
actagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaa
aaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgcttta
tttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaattta
gcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagta
ccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgg
gtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggct
ccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagc
cagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaaccca
cagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgt
ttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagatgg
aacaacgcataaccctgaaagattatgcaatgcgctttgggcaaaccaagacagctaaagatctcggcgtatatcaaag
cgcgatcaacaaggccattcatgcaggccgaaagatttttttaactataaacgctgatggaagcgtttatgcggaagag
gtaaagcccttcccgagtaacaaaaaaacaacagcataatactagatgtccagattagataaaagtaaagtgattaaca
gcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagca
gcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccat
actcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttac
taagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaatt
agcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggt
tgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattat
tacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcgg
attagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactga
tagtgctagtgtagatcactactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgc
ttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagg
gcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcat
gtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaag
tgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttca
tctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggc
ctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggc
cactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatca
agttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccgg
cggcatggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata