LOCUS       BBa_K177025             1226 bp    DNA     linear       06-Dec-2025
DEFINITION  GFP under araC promotor
ACCESSION   BBa_K177025
VERSION     BBa_K177025.1  
FEATURES             Location/Qualifiers
     CDS             1..78
                     /label=defunct araC
     stem_loop       22..37
                     /label=stem_loop
     stem_loop       57..72
                     /label=stem_loop
     promoter        1..183
                     /label=BBa_R0081
     misc_feature    195..220
                     /label=c-amp2
     misc_feature    197..235
                     /label=araO1
     misc_feature    234..263
                     /label=c-amp1
     misc_feature    264..292
                     /label=ara1 and ara2
     promoter        304..309
                     /label=-35
     promoter        327..332
                     /label=-10
     promoter        192..340
                     /label=BBa_R0080
     RBS             349..363
                     /label=RBS-1\Strong
     RBS             356..360
                     /label=RBS
     misc_feature    349..363
                     /label=BBa_B0030
     RBS             349..363
                     /label=BBa_B0030
     CDS             370..1089
                     /label=GFP protein
     CDS             370..1089
                     /label=BBa_E0040
     stem_loop       1109..1152
                     /label=stem_loop
     misc_feature    1098..1177
                     /label=BBa_B0010
     terminator      1098..1177
                     /label=BBa_B0010
     stem_loop       1193..1212
                     /label=T7 TE
     misc_feature    1219..1219
                     /label=stop
     polyA_site      1213..1226
                     /label=polya
     misc_feature    1186..1226
                     /label=BBa_B0012
     terminator      1186..1226
                     /label=BBa_B0012
ORIGIN
        1 caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca gagaagaaac
       61 caattgtcca tattgctaca gacattgccg tcactggtct ttactggctc ttctcgctaa
      121 ccaaaccggt aaccgcttat taaagcattc tgtaacaaag cgggaccaaa gccatgacaa
      181 aactactaga ggcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat
      241 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
      301 acctgacgct ttttatcgca actctctact gtttctccat tactagagat taaagaggag
      361 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      421 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      481 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      541 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
      601 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
      661 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
      721 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
      781 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
      841 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
      901 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
      961 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1021 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     1081 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1201 ttcgggtggg cctttctgcg tttata
//